Results 31 to 40 of about 1,388 (159)

Bacterial extracellular vesicles: A position paper by the microbial vesicles task force of the Chinese society for extracellular vesicles

Interdisciplinary Medicine, Volume 1, Issue 3, July 2023., 2023
Abstract Recently, the interest in extracellular vesicles released by bacteria has rapidly increased. Bacterial extracellular vesicles (BEVs) have been involved in bacteria‐bacteria and bacteria‐host interactions, which strengthen health or bring about various pathologies.
Minghui Wen, Jingyu Wang, Zihao Ou, Guangjun Nie, Ye Chen, Min Li, Zhiwei Wu, Sidong Xiong, Hongwei Zhou, Zifeng Yang, Gang Long, Jiacan Su, Han Liu, Yingying Jing, Zhenke Wen, Yuxuan Fu, Tieli Zhou, Hui Xie, Wenda Guan, Xi Sun, Zhang Wang, Juan Wang, Xunxun Chen, Linglei Jiang, Xiaolin Qin, Yicong Xue, Mei Huang, Xinyue Huang, Ruoru Pan, Haojun Zhen, Yuqi Du, Qianbei Li, Xixin Huang, Yuanyuan Wu, Pingping Wang, Kening Zhao, Bo Situ, Xiumei Hu, Lei Zheng   +38 more
wiley   +1 more source

Use of an EZ-Tn5-based random mutagenesis system to identify a novel toxin regulatory locus in Clostridium perfringens strain 13. [PDF]

PLoS ONE, 2009
BACKGROUND:Although useful for probing bacterial pathogenesis and physiology, current random mutagenesis systems suffer limitations for studying the toxin-producing bacterium Clostridium perfringens.
Jorge E Vidal, Jianming Chen, Jihong Li, Bruce A McClane   +3 more
doaj   +1 more source

Selective binding of perfringolysin O derivative to cholesterol-rich membrane microdomains (rafts) [PDF]

Proceedings of the National Academy of Sciences, 2001
There is increasing evidence that sphingolipid- and cholesterol-rich microdomains (rafts) exist in the plasma membrane. Specific proteins assemble in these membrane domains and play a role in signal transduction and many other cellular events.
A A, Waheed, Y, Shimada, H F, Heijnen, M, Nakamura, M, Inomata, M, Hayashi, S, Iwashita, J W, Slot, Y, Ohno-Iwashita   +8 more
openaire   +2 more sources

Cholesterol‐dependent cytolysins: The outstanding questions

IUBMB Life, Volume 74, Issue 12, Page 1169-1179, December 2022., 2022
Abstract The cholesterol‐dependent cytolysins (CDCs) are a major family of bacterial pore‐forming proteins secreted as virulence factors by Gram‐positive bacterial species. CDCs are produced as soluble, monomeric proteins that bind specifically to cholesterol‐rich membranes, where they oligomerize into ring‐shaped pores of more than 30 monomers ...
Bronte A. Johnstone, Riya Joseph, Michelle P. Christie, Craig J. Morton, Conall McGuiness, James C. Walsh, Till Böcking, Rodney K. Tweten, Michael W. Parker   +8 more
wiley   +1 more source

Raft platforms highly enriched in cholesterol: major scaffolds for IL‐6 signalling assembly with implications in inflammation and cancer

The FEBS Journal, Volume 289, Issue 19, Page 5891-5894, October 2022., 2022
Protospacer adjacent motif (PAM) recognition is crucial for the CRISPR/Cas9 system. The PAM‐interacting (PI) domain of Cas9 binds to PAM, determining the recognition specificity. However, additional Cas9 regions might affect PAM interaction. Here, Shaohua Yao and colleagues fused the PI domain of Cas9‐NG with a non‐PI fragment of xCas9, producing the ...
Faustino Mollinedo
wiley   +1 more source

Dynamic interspecies interactions and robustness in a four‐species model biofilm

MicrobiologyOpen, Volume 10, Issue 6, December 2021., 2021
Here, we describe the constitution of a model biofilm composed of four bacterial species, highly reproducible and robust against transient perturbations, in which we reveal a network of positive and negative interactions. Furthermore, we identified the molecular determinant of one negative interaction and demonstrated its importance for species ...
Aurélie Baliarda, Michèle Winkler, Laurent Tournier, Colin R. Tinsley, Stéphane Aymerich   +4 more
wiley   +1 more source

Molecular and cellular basis of microvascular perfusion deficits induced by Clostridium perfringens and Clostridium septicum.

PLoS Pathogens, 2008
Reduced tissue perfusion leading to tissue ischemia is a central component of the pathogenesis of myonecrosis caused by Clostridium perfringens. The C.
Michael J Hickey, Rain Y Q Kwan, Milena M Awad, Catherine L Kennedy, Lauren F Young, Pam Hall, Leanne M Cordner, Dena Lyras, John J Emmins, Julian I Rood   +9 more
doaj   +1 more source

Clostridium perfringens α-toxin action facilitates the Perfringolysin O-cholesterol interaction [PDF]

Biophysical Journal, 2009
Clostridium perfringens is a Gram-positive bacterium that causes gangrene and gastrointestinal disease in humans. These pathologies are mediated by potent extracellular protein toxins, particularly alpha-toxin (phospholipase C or PLC) and theta-toxin (perfringolysin O or PFO).
Moe, Paul C., Heuck, Alejandro P.
openaire   +1 more source

Immunoelectron Microscopic Localization of Cholesterol Using Biotinylated and Non-cytolytic Perfringolysin O [PDF]

Journal of Histochemistry & Cytochemistry, 2002
We used a proteolytically modified and biotinylated derivative of the cholesterol-binding θ-toxin (perfringolysin O) to localize cholesterol-rich membranes in cryosections of cultured human lymphoblastoid cells (RN) by electron microscopy. We developed a fixation and immunolabeling procedure to improve the preservation of membranes and minimize the ...
Wiebke, Möbius, Yoshiko, Ohno-Iwashita, Elly G, van Donselaar, Viola M J, Oorschot, Yukiko, Shimada, Toyoshi, Fujimoto, Harry F G, Heijnen, Hans J, Geuze, Jan W, Slot   +8 more
openaire   +2 more sources

Interaction of Macrophages and Cholesterol-Dependent Cytolysins: The Impact on Immune Response and Cellular Survival

Toxins, 2020
Cholesterol-dependent cytolysins (CDCs) are key virulence factors involved in many lethal bacterial infections, including pneumonia, necrotizing soft tissue infections, bacterial meningitis, and miscarriage.
Roshan Thapa, Sucharit Ray, Peter A. Keyel   +2 more
doaj   +1 more source