Results 61 to 70 of about 1,388 (159)

Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action

Scientific Reports, 2019
Ketamine is an antidepressant with rapid therapeutic onset and long-lasting effect, although the underlying mechanism(s) remain unknown. Using FRET-based nanosensors we found that ketamine increases [cAMP]i in astrocytes. Membrane capacitance recordings,
Eva Lasič, Marjeta Lisjak, Anemari Horvat, Mićo Božić, Aleksandra Šakanović, Gregor Anderluh, Alexei Verkhratsky, Nina Vardjan, Jernej Jorgačevski, Matjaž Stenovec, Robert Zorec   +10 more
doaj   +1 more source

Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens

Frontiers in Cellular and Infection Microbiology, 2023
Clostridium perfringens type A is the main cause of necrotic enteritis (NE) in chickens. Since the use of antibiotics in feed is withdrawn, it is imperative to find out suitable alternatives to control NE.
Jin Liu, Jin Liu, Shuangqi Wu, Shuangqi Wu, Honghao Zhao, Honghao Zhao, Chun Ye, Chun Ye, Shulin Fu, Shulin Fu, Yu Liu, Yu Liu, Ting Liu, Ting Liu, Yinsheng Qiu, Yinsheng Qiu   +15 more
doaj   +1 more source

Single molecule tracking the uncoupling of assembly and membrane insertion in Perfringolysin O [PDF]

, 2021
AbstractWe exploit single-molecule tracking and optical single channel recording in droplet interface bilayers to resolve the assembly pathway and pore-formation of the archetypical cholesterol-dependent cytolysin nanopore, Perfringolysin O. We follow the stoichiometry and diffusion of Perfringolysin O complexes during assembly with 60 millisecond ...
Michael J T Senior, Carina Monico, Eve E Weatherill, Robert J Gilbert, Alejandro P Heuck, Mark I Wallace   +5 more
openaire   +1 more source

The MICOS Complex Subunit Mic60 is Hijacked by Intracellular Bacteria to Manipulate Mitochondrial Dynamics and Promote Bacterial Pathogenicity

Advanced Science, Volume 11, Issue 46, December 11, 2024.
Upon the invasion of Listeria monocytogenes into a host cell, the primary virulence factor, listeriolysin O (LLO), secreted by the bacterium, has the capacity to breach the mitochondrial membrane, thereby gaining access to the inner mitochondrial membrane (IMM). The interaction between LLO and Mic60 can induce significant changes in the architecture of
Changyong Cheng, Mianmian Chen, Jing Sun, Jiali Xu, Simin Deng, Jing Xia, Yue Han, Xian Zhang, Jing Wang, Lei Lei, Ruidong Zhai, Qin Wu, Weihuan Fang, Houhui Song   +13 more
wiley   +1 more source

Matching Together Living Cells and Prototissues: Will There Be Chemistry?

ChemBioChem, Volume 25, Issue 18, September 16, 2024.
In the field of tissue engineering, the combination of synthetic and living tissues could be the turning point. In this work we review examples of hybrid protocell/living cell and prototissue/living cell systems, focusing on the chemistry that makes the integration between non‐living and living matter efficient.
Stefano Valente, Agostino Galanti, Edoardo Maghin, Nahid Najdi, Martina Piccoli, Pierangelo Gobbo   +5 more
wiley   +1 more source

Comment on ‘Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol’

eLife, 2018
The plasma membrane in mammalian cells is rich in cholesterol, but how the cholesterol is partitioned between the two leaflets of the plasma membrane remains a matter of debate. Recently, Liu et al. used domain 4 (D4) of perfringolysin O as a cholesterol
Kevin C Courtney, Karen YY Fung, Frederick R Maxfield, Gregory D Fairn, Xiaohui Zha   +4 more
doaj   +1 more source

Lactobacillus gasseri and Gardnerella vaginalis produce extracellular vesicles that contribute to the function of the vaginal microbiome and modulate host–Trichomonas vaginalis interactions

Molecular Microbiology, Volume 122, Issue 3, Page 357-371, September 2024.
Our study investigates the influence of extracellular vesicles, released by two bacterial species of the human cervicovaginal microbiome, on the infection by Trichomonas vaginalis. We described species‐specific protein cargoes of these vesicles matching the expected contribution of the bacteria to this biome.
Anastasiia Artuyants, Jiwon Hong, Priscila Dauros‐Singorenko, Anthony Phillips, Augusto Simoes‐Barbosa   +4 more
wiley   +1 more source

Genomic Insights into Virulence Factors and Multi-Drug Resistance in Clostridium perfringens IRMC2505A

Toxins, 2023
Clostridium perfringens is a spore-forming, Gram-positive anaerobic pathogen that causes several disorders in humans and animals. A multidrug-resistant Clostridium strain was isolated from the fecal sample of a patient who was clinically suspected of ...
Reem AlJindan, Doaa M. AlEraky, Maha Farhat, Noor B. Almandil, Sayed AbdulAzeez, Jesu Francis Borgio   +5 more
doaj   +1 more source

Contribution of Histidine Residues to Oligomerization of θ-Toxin (Perfringolysin O), a Cholesterol-Binding Cytolysin [PDF]

Bioscience, Biotechnology, and Biochemistry, 1999
Theta-toxin (perfringolysin O) modified by diethyl pyrocarbonate, a histidine-specific reagent, lost its hemolytic activity. The modified toxin retains the activities of binding to and insertion into cholesterol-containing membranes but lacks the ability to form oligomers.
M, Nakamura, N, Sekino-Suzuki, Y, Shimada, Y, Ohno-Iwashita   +3 more
openaire   +2 more sources

SR‐BI regulates the synergistic mast cell response by modulating the plasma membrane‐associated cholesterol pool

European Journal of Immunology, Volume 54, Issue 8, August 2024.
The plasma membrane cholesterol homeostasis is regulated by SR‐BI (gene name: Scarb1) in mast cells contributing to the synergistically enhanced pro‐inflammatory cytokine production upon co‐stimulation of the FcεRI by antigen together with receptors acting in synergy. Scarb1 deficiency results in reduced plasma membrane‐localized cholesterol leading to
Sandro Capellmann, Marlies Kauffmann, Michel Arock, Michael Huber   +3 more
wiley   +1 more source