Results 71 to 80 of about 2,525 (205)

Evaluation of the available cholesterol concentration in the inner leaflet of the plasma membrane of mammalian cells

Journal of Lipid Research, 2021
Cholesterol is an essential component of the mammalian plasma membrane involved in diverse cellular processes. Our recent quantitative imaging analysis using ratiometric cholesterol sensors showed that the available cholesterol concentration in the inner
Pawanthi Buwaneka, Arthur Ralko, Shu-Lin Liu, Wonhwa Cho   +3 more
doaj   +1 more source

Phylogenomic analysis of gastroenteritis-associated Clostridium perfringens in England and Wales over a 7-year period indicates distribution of clonal toxigenic strains in multiple outbreaks and extensive involvement of enterotoxin-encoding (CPE) plasmids [PDF]

, 2019
Clostridium perfringens is a major enteric pathogen known to cause gastroenteritis in human adults. Although major outbreak cases are frequently reported, only limited whole-genome sequencing (WGS) based studies have been performed to understand the ...
Amar, Corinne, Caim, Shabhonam, Dougan, Gordon, Hall, Lindsay J., Kiu, Raymond, Mather, Alison E., Painset, Anais, Pickard, Derek, Swift, Craig   +8 more
core   +1 more source

Several plant self‐incompatibility systems may be controlled by atypical receptor–ligand interactions

The Plant Journal, Volume 126, Issue 1, April 2026.
SUMMARY Self‐incompatibility (SI) is the single most important mechanism utilized by flowering plants to avoid self‐fertilization, thus preventing inbreeding and promoting outcrossing. Many plant SI systems are genetically controlled by a multi‐allelic S‐locus, containing two tightly linked genes that encode the female and male S‐determinants.
Zongcheng Lin, Maurice Bosch, Vernonica E. Franklin‐Tong   +2 more
wiley   +1 more source

Biotinylated θ-toxin derivative as a probe to examine intracellular cholesterol-rich domains in normal and Niemann-Pick type C1 cells

Journal of Lipid Research, 2003
BCθ is a proteolytically nicked and biotinylated derivative of a cholesterol binding protein perfringolysin O (θ-toxin), and has been used to detect cholesterol-rich domains at the plasma membrane (PM).
Shigeki Sugii, Patrick C. Reid, Nobutaka Ohgami, Yukiko Shimada, Robert A. Maue, Haruaki Ninomiya, Yoshiko Ohno-Iwashita, Ta-Yuan Chang   +7 more
doaj   +1 more source

The cholesterol-dependent cytolysin signature motif: a critical element in the allosteric pathway that couples membrane binding to pore assembly. [PDF]

PLoS Pathogens, 2012
The cholesterol-dependent cytolysins (CDCs) constitute a family of pore-forming toxins that contribute to the pathogenesis of a large number of Gram-positive bacterial pathogens.The most highly conserved region in the primary structure of the CDCs is the
Kelley J Dowd, Allison J Farrand, Rodney K Tweten   +2 more
doaj   +1 more source

Probing Genomic Aspects of the Multi-Host Pathogen Clostridium perfringens Reveals Significant Pangenome Diversity, and a Diverse Array of Virulence Factors [PDF]

, 2017
Clostridium perfringens is an important cause of animal and human infections, however information about the genetic makeup of this pathogenic bacterium is currently limited.
Lindsay J. Hall, Purnima Pachori, Raymond Kiu, Raymond Kiu, Sarah Alexander, Shabhonam Caim   +5 more
core   +2 more sources

Single molecule tracking the uncoupling of assembly and membrane insertion in Perfringolysin O [PDF]

, 2021
AbstractWe exploit single-molecule tracking and optical single channel recording in droplet interface bilayers to resolve the assembly pathway and pore-formation of the archetypical cholesterol-dependent cytolysin nanopore, Perfringolysin O. We follow the stoichiometry and diffusion of Perfringolysin O complexes during assembly with 60 millisecond ...
Michael J T Senior, Carina Monico, Eve E Weatherill, Robert J Gilbert, Alejandro P Heuck, Mark I Wallace   +5 more
openaire   +1 more source

A Bioorthogonal and Programmable Bacterial Delivery System for Spatiotemporally Targeted Therapy of Solid Tumors

Exploration, Volume 5, Issue 6, December 2025.
We developed a spatiotemporal targeting oncolytic bacterial therapy system, the STEPT (spatiotemporal targeting EcN‐pfo terminator) system, which injects azide hyaluronic acid hydrogel into tumors to capture DBCO‐labeled engineered Escherichia coli Nissle 1917 (EcN).
Yu‐Jia Wang, Wen‐Jie Jiang, Hua‐Jun Zhao, Jian‐Qun Deng, Yi‐Min Cai, Yi Li, Xiao‐Lin Meng, Jin Hou, Feng‐Shan Wang, Ju‐Zheng Sheng   +9 more
wiley   +1 more source

Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action

Scientific Reports, 2019
Ketamine is an antidepressant with rapid therapeutic onset and long-lasting effect, although the underlying mechanism(s) remain unknown. Using FRET-based nanosensors we found that ketamine increases [cAMP]i in astrocytes. Membrane capacitance recordings,
Eva Lasič, Marjeta Lisjak, Anemari Horvat, Mićo Božić, Aleksandra Šakanović, Gregor Anderluh, Alexei Verkhratsky, Nina Vardjan, Jernej Jorgačevski, Matjaž Stenovec, Robert Zorec   +10 more
doaj   +1 more source

Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens

Frontiers in Cellular and Infection Microbiology, 2023
Clostridium perfringens type A is the main cause of necrotic enteritis (NE) in chickens. Since the use of antibiotics in feed is withdrawn, it is imperative to find out suitable alternatives to control NE.
Jin Liu, Jin Liu, Shuangqi Wu, Shuangqi Wu, Honghao Zhao, Honghao Zhao, Chun Ye, Chun Ye, Shulin Fu, Shulin Fu, Yu Liu, Yu Liu, Ting Liu, Ting Liu, Yinsheng Qiu, Yinsheng Qiu   +15 more
doaj   +1 more source