Results 71 to 80 of about 1,422 (145)

High‐Resolution Patterned Delivery of Chemical Signals From 3D‐Printed Picoliter Droplet Networks

Advanced Materials, Volume 37, Issue 28, July 17, 2025.
3D‐printed picoliter droplet networks have been fabricated that control gene expression in bacterial populations by releasing chemical signals with precise spatial definition and high temporal resolution. This system of effector release is widely applicable, offering diverse applications in biology and medicine.
Jorin Riexinger, Thomas Caganek, Xingzao Wang, Yutong Yin, Khoa Chung, Linna Zhou, Hagan Bayley, Ravinash Krishna Kumar   +7 more
wiley   +1 more source

Nonclinical Development of BCG Replacement Vaccine Candidates

Vaccines, 2013
The failure of current Mycobacterium bovis bacille Calmette–Guérin (BCG) vaccines, given to neonates to protect against adult tuberculosis and the risk of using these live vaccines in HIV-infected infants, has emphasized the need for generating new, more
Bernd Eisele, Martin Gengenbacher, Reginald Kidd, David McCown, Sheldon Morris, Steven Derrick, David Hokey, Dominick Laddy, Rosemary Chang, Megan Fitzpatrick, Leander Grode, Kamalakannan Velmurugan, Stefan H.E. Kaufmann, John Fulkerson, Michael J. Brennan   +14 more
doaj   +1 more source

Genome sequencing and analysis of a type A Clostridium perfringens isolate from a case of bovine clostridial abomasitis. [PDF]

PLoS ONE, 2012
Clostridium perfringens is a common inhabitant of the avian and mammalian gastrointestinal tracts and can behave commensally or pathogenically. Some enteric diseases caused by type A C.
Victoria J Nowell, Andrew M Kropinski, J Glenn Songer, Janet I MacInnes, Valeria R Parreira, John F Prescott   +5 more
doaj   +1 more source

How interaction of perfringolysin O with membranes is controlled by sterol structure, lipid structure, and physiological low pH: insights into the origin of perfringolysin O-lipid raft interaction.

The Journal of biological chemistry, 2008
Perfringolysin O (PFO) is a sterol-dependent, pore-forming cytolysin. To understand the molecular basis of PFO membrane interaction, we studied its dependence upon sterol and lipid structure and aqueous environment. PFO interacted with diverse sterols, although binding was affected by double bond location in the sterol rings, sterol side chain ...
Lindsay D, Nelson, Arthur E, Johnson, Erwin, London   +2 more
openaire   +2 more sources

Light‐Triggered Protease‐Mediated Release of Actin‐Bound Cargo from Synthetic Cells

Advanced Biology, Volume 9, Issue 5, May 2025.
TEV Prtoease‐mediated Releasable Actin‐binding Protein (TRAP) is a protein‐based platform consisting of a cargo tightly bound to reconstituted actin networks in synthetic cells which can be proteolyticly released from the bound actin, followed by its secretion through membrane translocation mediated by a cell‐penetrating peptide.
Mousumi Akter, Hossein Moghimianavval, Gary D. Luker, Allen P. Liu   +3 more
wiley   +1 more source

BrnQ Branched-Chain Amino Acid Transporters Influence Toxin Production by, but Not Growth of, Clostridium perfringens Type A Strain ATCC3624

Toxins
By producing alpha toxin (PLC) and perfringolysin O (PFO), Clostridium perfringens type A strains are the most common cause of traumatic gas gangrene. C. perfringens cannot synthesize branched-chain amino acids (BCAAs), so BCAA transporters are essential
Jihong Li, Iman Mehdizadeh Gohari, Isabella Zhang, Bruce A. McClane   +3 more
doaj   +1 more source

Multiple Parameters Beyond Lipid Binding Affinity Drive Cytotoxicity of Cholesterol-Dependent Cytolysins

Toxins, 2018
The largest superfamily of bacterial virulence factors is pore-forming toxins (PFTs). PFTs are secreted by both pathogenic and non-pathogenic bacteria. PFTs sometimes kill or induce pro-pathogen signaling in mammalian cells, all primarily through plasma ...
Sucharit Ray, Roshan Thapa, Peter A. Keyel   +2 more
doaj   +1 more source

Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery

Advanced Science, Volume 12, Issue 15, April 17, 2025.
A modular endosomal escape system boosts transfection efficiency of a DNA delivery agent. This system comprises a phospholipase fused to a split‐calbindin domain and a nanobody targeting CD9. In the extracellular space, the phospholipase is tightly bound to protein‐DNA complexes, released within endosomes at low calcium concentrations and recruited to ...
Alexander Klipp, Christina Greitens, David Scherer, Alexander Elsener, Jean‐Christophe Leroux, Michael Burger   +5 more
wiley   +1 more source

Assessment of Brown Adipocyte Thermogenic Function by High-throughput Respirometry

Bio-Protocol, 2015
Brown adipose tissue (BAT) has the unique ability to dramatically increase mitochondrial uncoupled fuel oxidation for thermogenesis in response to adrenergic stimulation.
Kiana Mahdaviani, Ilan Benador, Orian Shirihai   +2 more
doaj   +1 more source

A biotinylated perfringolysin O derivative: A new probe for detection of cell surface cholesterol

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1997
theta-Toxin is a cholesterol-binding, pore-forming cytolysin of Clostridium perfringens. To detect cell surface cholesterol, we prepared a theta-toxin derivative, BC theta by biotinylation of a protease-nicked theta-toxin, which has the same binding affinity for cholesterol as theta-toxin without cytolytic activity.
Iwamoto, Machiko, Morita, Ikuo, Fukuda, Mitsugu, Murota, Sei-itsu, Ando, Susumu, Ohno-Iwashita, Yoshiko   +5 more
openaire   +2 more sources