Results 91 to 100 of about 1,422 (145)

Evidence for hypoxia‐induced dysregulated cholesterol homeostasis in preeclampsia: Insights into the mechanisms from human placental cells and tissues

The FASEB Journal, Volume 38, Issue 2, 31 January 2024.
This study provides a comprehensive overview of disrupted cholesterol homeostasis pathways in the pregnancy disorder preeclampsia. Additionally, we investigated a cell model designed to simulate this disorder by inducing continuous and intermittent hypoxic episodes.
Barbara Fuenzalida, Maria Jose Yañez, Martin Mueller, Hiten D. Mistry, Andrea Leiva, Christiane Albrecht   +5 more
wiley   +1 more source

Vav2 is a master regulator of repair against bacterial pore-forming toxins

Life Science Alliance
This one protein accounts for almost all Ca2+-dependent repair by activating at least 3 distinct pathways. Despite antibiotic therapy, 25–35% of patients with necrotizing soft tissue infections (NSTIs) die.
Victor Gbenga Kayejo, Ashlee Hensley, Tejal Katore, Peter A Keyel   +3 more
doaj   +1 more source

The presence of differentiated C2C12 muscle cells enhances toxin production and growth by Clostridium perfringens type A strain ATCC3624

Virulence
Clostridium perfringens type A causes gas gangrene, which involves muscle infection. Both alpha toxin (PLC), encoded by the plc gene, and perfringolysin O (PFO), encoded by the pfoA gene, are important when type A strains cause gas gangrene in a mouse ...
Jihong Li, Sameera Sayeed, Bruce A. McClane   +2 more
doaj   +1 more source

Immunogenic and neutralization efficacy of recombinant perfringolysin O of Clostridium perfringens and its C-terminal receptor-binding domain in a murine model. [PDF]

Immunol Res, 2022
Singh A, Rawat P, Choudhury D, Dixit A.
europepmc   +1 more source

Tracking cholesterol/sphingomyelin-rich membrane domains with the ostreolysin A-mCherry protein.

PLoS ONE, 2014
Ostreolysin A (OlyA) is an ∼15-kDa protein that has been shown to bind selectively to membranes rich in cholesterol and sphingomyelin. In this study, we investigated whether OlyA fluorescently tagged at the C-terminal with mCherry (OlyA-mCherry) labels ...
Matej Skočaj, Nataša Resnik, Maja Grundner, Katja Ota, Nejc Rojko, Vesna Hodnik, Gregor Anderluh, Andrzej Sobota, Peter Maček, Peter Veranič, Kristina Sepčić   +10 more
doaj   +1 more source

Dynamic Gene Expression Mitigates Mutational Escape in Lysis-Driven Bacteria Cancer Therapy

BioDesign Research
Engineered bacteria have the potential to deliver therapeutic payloads directly to tumors, with synthetic biology enabling precise control over therapeutic release in space and time.
Filippo Liguori, Nicola Pellicciotta, Edoardo Milanetti, Sophia Xi Windemuth, Giancarlo Ruocco, Roberto Di Leonardo, Tal Danino   +6 more
doaj   +1 more source

More than one way to bind to cholesterol: atypical variants of membrane-binding domain of perfringolysin O selected by ribosome display. [PDF]

RSC Adv, 2020
Šakanović A, Kranjc N, Omersa N, Podobnik M, Anderluh G.   +4 more
europepmc   +1 more source

In vitro evolution driven by epistasis reveals alternative cholesterol-specific binding motifs of perfringolysin O. [PDF]

J Biol Chem
Šakanović A, Kranjc N, Omersa N, Aden S, Kežar A, Kisovec M, Zavec AB, Caserman S, Gilbert RJC, Podobnik M, Crnković A, Anderluh G.   +11 more
europepmc   +1 more source

Use of mutant 125I-perfringolysin O to probe transport and organization of cholesterol in membranes of animal cells. [PDF]

Proc Natl Acad Sci U S A, 2013
Das A, Goldstein JL, Anderson DD, Brown MS, Radhakrishnan A.   +4 more
europepmc   +1 more source

Visualization of cholesterol deposits in lysosomes of Niemann-Pick type C fibroblasts using recombinant perfringolysin O. [PDF]

Orphanet J Rare Dis, 2014
Kwiatkowska K, Marszałek-Sadowska E, Traczyk G, Koprowski P, Musielak M, Lugowska A, Kulma M, Grzelczyk A, Sobota A.   +8 more
europepmc   +1 more source