Results 101 to 110 of about 7,690 (295)

Ceftriaxone Pulse Dosing Fails to Eradicate Biofilm-like Microcolony B. burgdorferi Persisters Which Are Sterilized by Daptomycin/Doxycycline/Cefuroxime Drug Combination without Pulse Dosing

Frontiers in Microbiology, 2016
Although the majority of Lyme disease patients can be cured, at least 10-20% of the patients continue to suffer from persisting symptoms such as fatigue, muscular and joint pain, and neurologic impairment after standard 2-4 week antibiotic treatment ...
Jie Feng, Shuo Zhang, Wanliang Shi, Ying Zhang   +3 more
doaj   +1 more source

Disrupting folate metabolism reduces the capacity of bacteria in exponential growth to develop persisters to antibiotics [PDF]

, 2018
Bacteria can survive high doses of antibiotics through stochastic phenotypic diversification. We present initial evidence that folate metabolism could be involved with the formation of persisters.
Morgan, Jasmine, Smith, Matthew, Mc Auley, Mark T, Salcedo-Sora, J. Enrique, Enrique Salcedo-Sora, J., Mc Auley, Mark T.   +5 more
core   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Novel Glycopolymer Eradicates Antibiotic- and CCCP-Induced Persister Cells in Pseudomonas aeruginosa

Frontiers in Microbiology, 2018
Antibiotic treatments often fail to completely eradicate a bacterial infection, leaving behind an antibiotic-tolerant subpopulation of intact bacterial cells called persisters. Persisters are considered a major cause for treatment failure and are thought
Vidya P. Narayanaswamy, Laura L. Keagy, Kathryn Duris, William Wiesmann, Allister J. Loughran, Stacy M. Townsend, Shenda Baker   +6 more
doaj   +1 more source

Single-cell Raman spectroscopy identifies E. coli persisters and their functionalities

, 2021
Objectives: Microbial persisters are the featured tiny sub-population of microorganisms highly tolerant to multiple antimicrobials. Currently, studies on persisters remain challenging owing to technical limitations.
Xu, J, Jin, L, Chen, R, Wang, C
core  

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Intoxication of antibiotic persisters by host RNS inactivates their efflux machinery during infection.

PLoS Pathogens
The host environment is of critical importance for antibiotic efficacy. By impacting bacterial machineries, stresses encountered by pathogens during infection promote the formation of phenotypic variants that are transiently insensitive to the action of ...
Séverin Ronneau, Charlotte Michaux, Rachel T Giorgio, Sophie Helaine   +3 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Supplementary Material for: Staphylococcus aureus Persisters Tolerant to Bactericidal Antibiotics

, 2017
Bacterial persister cells are non- or slow-growing reversible phenotypic variants of the wild type, tolerant to bactericidal antibiotics. We analyzed here Staphylococcus aureus persister levels by monitoring colony-forming unit counts of planktonically ...
Bertram R. (3835897), Lewis K. (3835894), Lechner S. (3835891)   +2 more
core   +1 more source