Molecular typing of MRSA isolates by spa and PFGE
Abstract Background Methicillin resistant S. aureus (MRSA) strains circulating among populations and crossing borders constitute a major problem for health control and require a fast and simple genotypic approach. Methods In the current study we compared staphylococcal protein A (spa) and pulsed field gel electrophoresis (PFGE) typing methods to
Khaled R. Alkharsah +5 more
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Two major outbreaks of invasive meningococcal disease serogroup C (IMD-C) were identified in British Columbia between 2000 and 2004. Pulsed-field gel electrophoresis (PFGE) and porA gene sequencing of all retained IMD-C isolates were used to assess ...
Grahame Quan +8 more
doaj +1 more source
Insertion/deletion-related polymorphisms in the human T cell receptor beta gene complex. [PDF]
Insertion/deletion related polymorphisms (IDRP) involving stretches of 15-30 kb within the human TCR-beta gene complex were revealed by pulse-field gel electrophoresis.
Hauser, SL +3 more
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Methods of isolation and identification of pathogenic and potential pathogenic bacteria from skins and tannery effluents [PDF]
Currently there is no standard protocol available within the leather industry to isolate and identify pathogenic bacteria from hides, skins or tannery effluent.
Allen, Stuart C H +4 more
core
PFGE and YAC analysis of the Arabidopsis genome
Procedures for the preparation, analysis, and cloning of large DNA molecules from Arabidopsis thaliana are described. Root callus protoplasts are used to prepare large DNA molecules in agarose plugs or in liquid. Large DNA preparations can be used both for restriction enzyme analysis of the Arabidopsis genome and for molecular cloning of large segments
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Analysis of the Essential Functions of the C-terminal Protein/Protein Interaction Domain of Saccharomyces cerevisiae pol epsilon and Its Unexpected Ability to Support Growth in the Absence of the DNA Polymerase Domain [PDF]
As first observed by Wittenberg (Kesti, T., Flick, K., Keranen, S., Syvaoja, J. E., and Wittenburg, C. (1999) Mol. Cell 3, 679-685), we find that deletion mutants lacking the entire N-terminal DNA polymerase domain of yeast pol epsilon are viable ...
Campbell, Judith L. +2 more
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Molecular Genetic Typing of Staphylococcus aureus from Cows, Goats, Sheep, Rabbits and Chickens [PDF]
End of project reportsS. aureus can also cause a number of infections in animals such as tick-associated pyaemia in lambs, staphylococcosis in rabbits, septicaemia, abscesses and chondronecrosis in chickens and pneumonia and osteomyelitis complex in ...
Hartigan, Patrick J. +3 more
core
CRISPR-associated sequence diversity within Xanthomonas albilineans, the causal agent of leaf scald disease of sugarcane [PDF]
Co-ordinated efforts to collect and maintain cotton genetic resources have increased over the last 100 years to insure the worldwide economic value of cotton fibre and cotton by-products.
Bolot, Stéphanie +7 more
core
PFGE of human DNA: 5-azacytidine improves restriction
C, Dobkin, C, Ferrando, W, Brown
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