Results 121 to 130 of about 11,559 (161)

Construction of Lambda Libraries from Large PFGE Fragments

2003
Pulsed-field gel electrophoresis (PFGE) has the capacity to fractionate large fragments of DNA up to thousands of kilobases in size. This aspect of the technique has been exploited for constructing long-range restriction maps of chromosomes from many different species including humans (see Chapters 14 , 15 , and 18 ).
C, Pritchard, M, Burmeister
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Wilms' tumor‐specific methylation pattern in 11p13 detected by PFGE

Genes, Chromosomes and Cancer, 1992
AbstractThe analysis of 2,550 kb from 11 p 13 in Wilms' tumor (WT) material revealed two regions that differed significantly in their methylation between tumor and normal tissue. In WT a hypomethylated area defined by an Nrul and Mlul recognition site 100–150 kb centromeric of the WT gene WTI (site A) and hypermethylation defined by an Nrul and Sacll ...
B, Royer-Pokora, S, Schneider
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Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes

2020
PFGE is a valuable tool for assessing L. monocytogenes strain interrelatedness. It is based on the study of total bacterial DNA restriction patterns. Cells are embedded in agarose plugs before being lysed. The released DNA is then digested into large fragments by restriction enzymes.
Karen, Hunt, Kieran, Jordan
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PFGE analysis of yeast artificial chromosomes

1995
Abstract There are two basic pulsed field gel electrophoresis (PFGE)-related techniques for mapping yeast artificial chromosomes (YACs). One is to digest the DNA with restriction endonucleases, detect specific fragments with defined probes, and construct a restriction map.
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Yeast artificial chromosomes cloning using PFGE

1995
Abstract The major goal of the human genome project includes the isolation of the entire human genome in overlapping clones and the development of physical maps of the cloned DNA. Cloning into yeast artificial chromosomes (YAC) represents the method of choice for genome mapping analysis in the megabase range.
P Ougen, D Cohen
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Analysis of the genomes of protozoan parasites using PFGE

1995
Abstract Pulsed field gel electrophoresis (PFGE) has played a major role in developing our current knowledge of the genomes of a number of protozoan parasites as the genomes are small and the chromosomes usually fall within a usable size range. The studies on malaria and trypanosomatids described here demonstrate the applicability of the
David J Kemp, Roberto Cappai
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Introduction to Pulsed-Field Gel Electrophoresis (PFGE)

1999
An agarose gel is made up of pores of various sizes with net pore size varying with concentration of the matrix. The mobility of a DNA molecule depends on how easily it can pass through these pores. Larger molecules must change their conformation to get through the smaller pores and thus migrate more slowly.
Feng Qian, Gregory G. Germino
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Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes

2014
PFGE is a valuable tool for assessing Listeria monocytogenes strain interrelatedness. It is based on the study of total bacterial DNA restriction patterns. Cells are embedded in agarose plugs before being lysed. The released DNA is then digested into large fragments by restriction enzymes.
Dalmasso, Marion, Jordan, Kieran
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PFGE MAPPER and PFGE READER: two tools to aid in the analysis and data input of pulse field gel electrophoresis maps.

Proceedings. Symposium on Computer Applications in Medical Care, 1993
Pulse field gel electrophoresis mapping is an important technique for characterizing large segments of DNA. We have developed two tools to aid in the construction of pulse field electrophoresis gel maps: PFGE READER which stores experimental conditions and calculates fragment sizes and PFGE MAPPER which constructs pulse field gel electrophoresis maps.
M A, Shifman, P, Nadkarni, P L, Miller
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A method for overcoming DNA degradation during PFGE for Serratia marcescens

Journal of Microbiological Methods, 2011
The DNA of some bacteria is broken up by Tris-dependent endonuclease activity during the process of sample preparation for pulsed field gel electrophoresis (PFGE). Adding thiourea to the electophoresis buffer for isolates that exhibit DNA degradation has been the method used for many bacterial genera.
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