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PFGE: Importance in Food Quality
Recent Patents on Food, Nutrition & Agriculture, 2009 In late 19 century, great interest has arisen for food quality. This is referred as absence of pathogens in food (safety for consumers) and as nutritional quality of food (organoleptic characteristics). Pulsed-field gel electrophoresis (PFGE) is, among the molecular techniques developed in the last years, one of the most reliable, discriminative and ...
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Rapid PFGE method for fingerprinting of Serratia marcescens isolates
Journal of Microbiological Methods, 2009We modified the conventional PFGE procedure for Serratia marcescens to establish a rapid method. Our protocol showed modification in the bacterial lysis, washing, and restriction enzyme digestion time. This resulted in shortening the time needed by about 3 days compared to the conventional PFGE method.
Marie Fe F Bohol +2 more
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PFGE Protocols to Distinguish Subspecies of Lactococcus lactis
, 2015 Pulsed-field gel electrophoresis (PFGE), developed in the mid-1980s, rapidly became a "gold standard" method for analyzing bacterial chromosomes. Today, although outcompeted in resolution by alternative methods, such as optical mapping, and not applicable for high-throughput analyses, PFGE remains a valuable method for bacterial strain typing. Here, we
Le Bourgeois, Pascal +5 more
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Journal of Hospital Infection, 2005 A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to 50 isolates of the UK epidemic strain of Clostridium difficile, polymerase chain reaction (PCR) ribotype 001, to develop a PFGE-based subtyping scheme. This protocol
Jon S Brazier
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Localization of yeast glucoamylase genes by PFGE and OFAGE
Current Genetics, 1988Chromosomes of two closely related yeast strains, the amylolytic Saccharomyces diastaticus and the non-amylolytic Saccharomyces cerevisiae, were resolved by pulsed field gel electrophoresis (PFGE) and orthological field alteration gel electrophoresis (OFAGE). Electrophoretic karyotypes of these two strains are identical.
Pretorius I.S., Marmur J.
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Methods, 1990
Pulsed-field gel electrophoresis (PFGE) is becoming an increasingly important tool in genetic mapping. The physical maps generated through PFGE techniques complement the classical genetic maps and provide a wider range of markers for mapping. PFGE is also used in conjunction with long-range cloning techniques, especially those based on rare-cutter ...
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Pulsed-field gel electrophoresis (PFGE) is becoming an increasingly important tool in genetic mapping. The physical maps generated through PFGE techniques complement the classical genetic maps and provide a wider range of markers for mapping. PFGE is also used in conjunction with long-range cloning techniques, especially those based on rare-cutter ...
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1995
Abstract The development of pulsed field gel electrophoresis (PFGE), which allows the separation of DNA molecules as large as 10 Mb, has enabled large regions of genomic DNA to be mapped and analysed without cloning the DNA (1–3).
M F Ho, A P Monaco
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Abstract The development of pulsed field gel electrophoresis (PFGE), which allows the separation of DNA molecules as large as 10 Mb, has enabled large regions of genomic DNA to be mapped and analysed without cloning the DNA (1–3).
M F Ho, A P Monaco
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Mutation detection and diagnosis Using PFGE
1995Abstract Pulse field gel electrophoresis (PFGE) was specifically designed to separate large DNA fragments (1). Consequently, the great innovation of PFGE analysis for mutation identification and diagnosis was its range of detection and its ability to scan for genetic rearrangements at large distances from a given locus (probe).
den Dunnen, J.T. +3 more
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A constitutional mutation within the retinoblastoma gene detected by PFGE
Clinical Genetics, 1994Retinoblastoma may be caused by constitutional mutations in the retinoblastoma gene which segregates as an autosomal dominant inherited predisposition for developing retinoblastoma tumours. Since 75% of these cases are new mutations, there is a need for methods to identify carriers of such germ‐line mutations, so that informed genetic counselling is ...
M, Janson, M, Nordenskjöld
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