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Introduction to Pulsed-Field Gel Electrophoresis (PFGE)
1999An agarose gel is made up of pores of various sizes with net pore size varying with concentration of the matrix. The mobility of a DNA molecule depends on how easily it can pass through these pores. Larger molecules must change their conformation to get through the smaller pores and thus migrate more slowly.
Feng Qian, Gregory G. Germino
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A constitutional mutation within the retinoblastoma gene detected by PFGE
Clinical Genetics, 1994Retinoblastoma may be caused by constitutional mutations in the retinoblastoma gene which segregates as an autosomal dominant inherited predisposition for developing retinoblastoma tumours. Since 75% of these cases are new mutations, there is a need for methods to identify carriers of such germ‐line mutations, so that informed genetic counselling is ...
M, Janson, M, Nordenskjöld
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Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes
2014PFGE is a valuable tool for assessing Listeria monocytogenes strain interrelatedness. It is based on the study of total bacterial DNA restriction patterns. Cells are embedded in agarose plugs before being lysed. The released DNA is then digested into large fragments by restriction enzymes.
Dalmasso, Marion, Jordan, Kieran
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PFGE analysis of yeast artificial chromosomes
1995Abstract There are two basic pulsed field gel electrophoresis (PFGE)-related techniques for mapping yeast artificial chromosomes (YACs). One is to digest the DNA with restriction endonucleases, detect specific fragments with defined probes, and construct a restriction map.
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Proceedings. Symposium on Computer Applications in Medical Care, 1993
Pulse field gel electrophoresis mapping is an important technique for characterizing large segments of DNA. We have developed two tools to aid in the construction of pulse field electrophoresis gel maps: PFGE READER which stores experimental conditions and calculates fragment sizes and PFGE MAPPER which constructs pulse field gel electrophoresis maps.
M A, Shifman, P, Nadkarni, P L, Miller
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Pulse field gel electrophoresis mapping is an important technique for characterizing large segments of DNA. We have developed two tools to aid in the construction of pulse field electrophoresis gel maps: PFGE READER which stores experimental conditions and calculates fragment sizes and PFGE MAPPER which constructs pulse field gel electrophoresis maps.
M A, Shifman, P, Nadkarni, P L, Miller
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Elektroforeza DNA u promjenjivom električnom polju (PFGE)
2007Opisan je postupak za razgradnju visokomolekularne DNA restrikcijskim endonukleazama u agaroznim blokićima te postupak elektroforeze DNA u promjenjivom električnom polju.
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