A newly discovered fluorogenic DNAzyme, RFD‐CD3, enables rapid and sensitive detection of Clostridioides difficile and—unexpectedly—Listeria monocytogenes, making it the first dual‐responsive DNAzyme for two unrelated bacterial pathogens. Its strong catalytic efficiency, pH‐tolerant activity, and modular trans‐acting design position it as a promising ...
Soyeon Lee +4 more
wiley +1 more source
Positive regulation of meiotic DNA double-strand break formation by activation of the DNA damage checkpoint kinase Mec1(ATR) [PDF]
During meiosis, formation and repair of programmed DNA double-strand breaks (DSBs) create genetic exchange between homologous chromosomes-a process that is critical for reductional meiotic chromosome segregation and the production of genetically diverse ...
Allison, Rachal M +4 more
core +1 more source
Electroelution Into a Salt Trap: Reviving an Old‐School Approach to DNA Purification
Electroelution into a salt cushion is a well‐established yet underutilized method of capturing gel‐purified nucleic acids. This Perspective discusses how this traditional method can be reengineered to meet the demands of next‐generation technologies, such as long‐read sequencing.
Ruslan Kalendar +4 more
wiley +1 more source
Development of Multi-Locus Variable Number Tandem Repeat Analysis for Outbreak Detection of Neisseria meningitidis [PDF]
Neisseria meningitidis is a major cause of septicemia and meningitis worldwide. Traditional typing methods like pulsed-field gel electrophoresis (PFGE) for identifying outbreaks are subjective and time consuming.
Price, Alicia Anne
core
Genomic analysis of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex by isoenzyme analysis and pulsed-field gel electrophoresis. [PDF]
Initially, multilocus enzyme electrophoresis was used to examine genetic relationships among 63 isolates of Mycobacterium bovis and 13 other members of the M. tuberculosis complex.
Cousins, D.V. +3 more
core +2 more sources
ABSTRACT The California almond industry underwent a remarkable transformation in food safety management and culture following outbreaks of salmonellosis associated with the consumption of raw almonds in 2000–2001 and 2003–2004. However, limited studies have examined these changes from a longitudinal perspective.
Han Chen +4 more
wiley +1 more source
Characterization of an emergent clone of enteroinvasive Escherichia coli circulating in Europe [PDF]
Enteroinvasive Escherichia coli (EIEC) cause intestinal illness indistinguishable from that caused by Shigella, mainly in developing countries. Recently an upsurge of cases of EIEC infections has been observed in Europe, with two large outbreaks ...
Caprioli, A. +9 more
core +1 more source
Molecular characterization and antimicrobial-resistance gene profile of Staphylococcus aureus strains isolated from ovine mastitis in Jordan [PDF]
Background and Aim: Ovine mastitis, particularly subclinical mastitis caused by Staphylococcus aureus, poses significant economic and health challenges in livestock management.
Mohammad H. Gharaibeh +4 more
doaj +1 more source
This scoping review with textual analysis maps antimicrobial resistance in Salmonella across aquaculture, seafood, and related aquatic environments. Across 93 studies, recurrent MDR patterns suggest sentinel serotypes (S. Typhimurium, S. Newport) and a minimal antimicrobial susceptibility testing (AST) panel to guide matrix‐stratified One Health ...
Cristiane Coimbra de Paula +8 more
wiley +1 more source
Massive Increase, Spread, and Exchange of Extended Spectrum {beta}-Lactamase-Encoding Genes Among Intestinal Enterobacteriaceae in Hospitalized Children With Severe Acute Malnutrition in Niger. [PDF]
Background. From the time of CTX-M emergence, extended-spectrum β-lactamase-producing enterobacteria (ESBL-E) have spread worldwide in community settings as well as in hospitals, particularly in developing countries.
Andremont, A +13 more
core +2 more sources

