Results 111 to 120 of about 167 (163)
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Phages as delivery vehicles and phage display
2023Bacteriophages (Phages in short) were introduced as the natural enemy of bacteria that may act as alternatives to antibiotics to overcome the challenge of antibiotic resistance. However, in the recent history of science, phages have been employed in different molecular tools and used in numerous therapeutic and diagnostic approaches.
Salsabil, Makky +4 more
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Immunotechnology, 1995
Phage display is a powerful method for the selection and evolution of proteins and peptides. Applications include the generation of potent and novel antibodies, the in vitro improvement of protein affinity and function, epitope discovery, the development of leads for vaccine research and the identification of interacting proteins using cDNA libraries.
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Phage display is a powerful method for the selection and evolution of proteins and peptides. Applications include the generation of potent and novel antibodies, the in vitro improvement of protein affinity and function, epitope discovery, the development of leads for vaccine research and the identification of interacting proteins using cDNA libraries.
openaire +2 more sources
2017
Metasecretome is a collection of cell-surface and secreted proteins that mediate interactions between microbial communities and their environment. These include adhesins, enzymes, surface structures such as pili or flagella, vaccine targets or proteins responsible for immune evasion.
Dragana Gagic +4 more
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Metasecretome is a collection of cell-surface and secreted proteins that mediate interactions between microbial communities and their environment. These include adhesins, enzymes, surface structures such as pili or flagella, vaccine targets or proteins responsible for immune evasion.
Dragana Gagic +4 more
openaire +3 more sources
2016
Chemokines are small chemoattractant proteins involved in the recruitment of leukocytes to the site of inflammation. Due to their prominent role in the inflammatory process, chemokine inhibitors have been developed by parasites to remain undetected not only by the host immune system but also by various laboratories to develop anti-inflammatory ...
Steven M. Dunn +3 more
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Chemokines are small chemoattractant proteins involved in the recruitment of leukocytes to the site of inflammation. Due to their prominent role in the inflammatory process, chemokine inhibitors have been developed by parasites to remain undetected not only by the host immune system but also by various laboratories to develop anti-inflammatory ...
Steven M. Dunn +3 more
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2017
ORFeome phage display allows the efficient functional screening of entire proteomes or even metaproteomes to identify immunogenic proteins. For this purpose, randomly fragmented, whole genomes or metagenomes are cloned into a phage-display vector allowing positive selection for open reading frames (ORF) to improve the library quality.
Michael Hust +3 more
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ORFeome phage display allows the efficient functional screening of entire proteomes or even metaproteomes to identify immunogenic proteins. For this purpose, randomly fragmented, whole genomes or metagenomes are cloned into a phage-display vector allowing positive selection for open reading frames (ORF) to improve the library quality.
Michael Hust +3 more
openaire +3 more sources
2000
The development of monoclonal antibody (mAb) technology (1) has had a significant impact on many fields of research, in particular immunology. However, the method has limitations. The use of recombinant DNA technology and demonstration by Smith 1985 (2) that peptides can be expressed on the surface of filamentous bacteriophages have permitted the ...
Palmer Db, Michael M. Johns
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The development of monoclonal antibody (mAb) technology (1) has had a significant impact on many fields of research, in particular immunology. However, the method has limitations. The use of recombinant DNA technology and demonstration by Smith 1985 (2) that peptides can be expressed on the surface of filamentous bacteriophages have permitted the ...
Palmer Db, Michael M. Johns
openaire +6 more sources
Phage display and pharmacogenomics
Pharmacogenomics, 2000Surface display of genetic diversity is a technology that can produce specific binding agents for almost any target molecule, and is especially well suited for making agents that bind specific proteins. Until now, pharmacogenomic studies have followed the response of cells to drugs and other agents by tracking the mRNAs that encode the proteins of ...
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Phage Display of Antibody Fragments
Current Protein and Peptide Science, 2000In recent years, phage display of peptides and proteins has become a very popular method in oncology, immunology, protein engineering and ligand-receptor studies among others. Antibody fragments, as Fabs or single chain Fv, have been among the first proteins to be displayed on the surface of a filamentous bacteriophage with a procedure initially ...
PINI, ALESSANDRO, BRACCI, LUISA
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2008
Molecular imaging is at the forefront in the advancement of in-vivo diagnosis and monitoring of cancer. New peptide-based molecular probes to facilitate cancer detection are rapidly evolving. Peptide-based molecular probes that target apoptosis, angiogenesis, cell signaling and cell adhesion events are in place. Bacteriophage (phage) display technology,
Susan L. Deutscher, Jessica R. Newton
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Molecular imaging is at the forefront in the advancement of in-vivo diagnosis and monitoring of cancer. New peptide-based molecular probes to facilitate cancer detection are rapidly evolving. Peptide-based molecular probes that target apoptosis, angiogenesis, cell signaling and cell adhesion events are in place. Bacteriophage (phage) display technology,
Susan L. Deutscher, Jessica R. Newton
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Phage Display of RNase A and an Improved Method for Purification of Phages Displaying RNases
Biological Chemistry, 2000Functional ribonuclease A was presented on the surface of the filamentous phage M13 by fusion to the minor coat protein. RNase activity of the fusion protein was shown by a zymogram assay. In addition, we established a modified method for preparing RNase-displaying phages without contaminating host RNases.
Hans-Heinrich Foerster +2 more
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