Results 121 to 130 of about 167 (163)
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2012
Phage display has emerged as one of the leading technologies for the selection and generation of highly specific antibodies, offering a number of advantages over traditional ways of antibody generation such as mouse hybridoma techniques. While there are various possibilities to conduct phage display, selection of antibodies via solution panning is an ...
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Phage display has emerged as one of the leading technologies for the selection and generation of highly specific antibodies, offering a number of advantages over traditional ways of antibody generation such as mouse hybridoma techniques. While there are various possibilities to conduct phage display, selection of antibodies via solution panning is an ...
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Phage Displayed Peptide Libraries
Combinatorial Chemistry & High Throughput Screening, 1999Peptide libraries may be constructed by grafting, in vitro, random DNA sequences into a carrier gene and then introducing the degenerate hybrid coding sequence into an expression organism. This review will focus on phage display, which was the first expression organism for peptide library expression to be described and which still maintains ...
Cesareni G, Castagnoli L, Cestra G
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[6] Immunization with phage-displayed mimotopes
1996Publisher Summary Phage-displayed mimotopes (phagotopes) are selected from random peptide libraries, because they bind to specific antibodies, thus behaving as antigenic mimics that resemble to some extent the original antigen. If the positive image of the antigen (Ag) these mimotopes provide is accurate enough to induce the production of specific ...
GALFREĢ G +5 more
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Immunology & Cell Biology, 2002
Phage display provides a powerful technique for rapidly selecting a particular binding clone from millions of other irrelevant clones. One of the first applications of this technology was in the display and isolation of monoclonal antibodies. More recently, phage display technology has been increasingly used in other areas of research, such as in the ...
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Phage display provides a powerful technique for rapidly selecting a particular binding clone from millions of other irrelevant clones. One of the first applications of this technology was in the display and isolation of monoclonal antibodies. More recently, phage display technology has been increasingly used in other areas of research, such as in the ...
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2003
Bacteriophage display systems have been developed to allow the selection of a protein or peptide based on its ability to bind to a selected target molecule. The antibody-display approach uses filamentous phage that express combinations of randomly assembled pairs of heavy and light chain genes either as a single chain antibody fragment or fragment ...
PALMER DB, DE LORENZO, CLAUDIA
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Bacteriophage display systems have been developed to allow the selection of a protein or peptide based on its ability to bind to a selected target molecule. The antibody-display approach uses filamentous phage that express combinations of randomly assembled pairs of heavy and light chain genes either as a single chain antibody fragment or fragment ...
PALMER DB, DE LORENZO, CLAUDIA
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Fibrinolysis, 1994
Filamentous phage display of peptides, proteins, and protein fragments is a powerful method for the selection of functional clones from vast libraries. Over the past few years this approach has been utilized to probe the binding sites of receptors and enzymes and to make the hybridoma approach to monoclonal antibodies obsolete.
Carlos F. Barbas, Shana M. Barbas
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Filamentous phage display of peptides, proteins, and protein fragments is a powerful method for the selection of functional clones from vast libraries. Over the past few years this approach has been utilized to probe the binding sites of receptors and enzymes and to make the hybridoma approach to monoclonal antibodies obsolete.
Carlos F. Barbas, Shana M. Barbas
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2005
Abstract Phage display is a process by which a peptide or a protein is expressed as an exterior fusion to a surface protein of a phage particle. The peptide or protein sequence can be deduced from its encoding DNA sequence that resides in the phage particle or in a transductant.
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Abstract Phage display is a process by which a peptide or a protein is expressed as an exterior fusion to a surface protein of a phage particle. The peptide or protein sequence can be deduced from its encoding DNA sequence that resides in the phage particle or in a transductant.
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2017
Antibody phage display has become an indispensable tool for the discovery and optimization of target-specific monoclonal antibodies suitable for demanding applications including therapeutic reagents. The in vitro nature of the technology enables the rapid and efficient identification of specific binders, as well as greater control over selection ...
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Antibody phage display has become an indispensable tool for the discovery and optimization of target-specific monoclonal antibodies suitable for demanding applications including therapeutic reagents. The in vitro nature of the technology enables the rapid and efficient identification of specific binders, as well as greater control over selection ...
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Using Phage Display in Neurobiology
Current Protocols in Neuroscience, 2002AbstractIn this unit some of the basic protocols involved in the manipulation of phage display libraries are described, including the rescue and amplification of such libraries, selection and screening from them and testing of derived clones.
Daniele Sblaterro +5 more
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Phage Display and Selections on Cells
2012Traditional methods of phage display panning bind purified antigen to plates or other solid phases to which libraries are then applied. These methods are not directly applicable to antigens in their native environment on cell surfaces or in settings where the target antigen is unknown.
Patrick Chames, Klervi Even-Desrumeaux
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