Results 251 to 260 of about 978,488 (287)
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Phage Display of RNase A and an Improved Method for Purification of Phages Displaying RNases
Biological Chemistry, 2000Functional ribonuclease A was presented on the surface of the filamentous phage M13 by fusion to the minor coat protein. RNase activity of the fusion protein was shown by a zymogram assay. In addition, we established a modified method for preparing RNase-displaying phages without contaminating host RNases.
K, Korn, H H, Foerster, U, Hahn
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Immunization with phage-displayed mimotopes.
Methods in enzymology, 1996Phage-displayed mimotopes (phagotopes) are selected from random peptide libraries, because they bind to specific antibodies, thus behaving as antigenic mimics that resemble to some extent the original antigen. If the positive image of the antigen (Ag) these mimotopes provide is accurate enough to induce the production of specific antibodies (Ab) in ...
GALFRÈ G +5 more
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Combinatorial Chemistry & High Throughput Screening, 2012
Shotgun phage display cloning is a useful tool for studying interactions between bacterial and host proteins. Libraries are constructed by cloning randomly fragmented prokaryotic DNA into phage mid-vectors. Theoretically, these libraries will consist of phages that together display all proteins encoded by the bacterial genome.
K, Jacobsson, L, Frykberg
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Shotgun phage display cloning is a useful tool for studying interactions between bacterial and host proteins. Libraries are constructed by cloning randomly fragmented prokaryotic DNA into phage mid-vectors. Theoretically, these libraries will consist of phages that together display all proteins encoded by the bacterial genome.
K, Jacobsson, L, Frykberg
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Affinity Maturation by Phage Display
2008Antibodies are indispensable tools for research, diagnostics, and therapy. However, sometimes antibodies with the most favourable specificity profile lack sufficient affinity for a desired application. Here, we describe a method to increase the affinity of recombinant scFv antibody fragments based on random mutagenesis and phage display under stringent
Holger, Thie +4 more
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2005
Abstract Phage display is a process by which a peptide or a protein is expressed as an exterior fusion to a surface protein of a phage particle. The peptide or protein sequence can be deduced from its encoding DNA sequence that resides in the phage particle or in a transductant.
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Abstract Phage display is a process by which a peptide or a protein is expressed as an exterior fusion to a surface protein of a phage particle. The peptide or protein sequence can be deduced from its encoding DNA sequence that resides in the phage particle or in a transductant.
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Functional cloning by phage display
Biochimie, 2008This review focusses on the isolation of proteins from genomic or cDNA expression products libraries displayed on phage. The use of phage display is highlighted for the characterization of binding proteins with diverse biological functions. Phage display is compared with another strategy, the yeast two-hybrid method.
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Phage display and other peptide display technologies.
FEMS Microbiology Reviews, 2022Lidia Gaffke +2 more
exaly
2004
Abstract Phage display has become established as a powerful protein engineering method for identifying polypeptides with novel properties, and altering the properties of existing ones. Although the technique is widely used in biological research and drug discovery, it remains technically challenging, and new applications and procedures ...
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Abstract Phage display has become established as a powerful protein engineering method for identifying polypeptides with novel properties, and altering the properties of existing ones. Although the technique is widely used in biological research and drug discovery, it remains technically challenging, and new applications and procedures ...
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