Improved single-swab sample preparation for recovering bacterial and phage DNA from human skin and wound microbiomes. [PDF]
, 2019 BackgroundCharacterization of the skin and wound microbiome is of high biomedical interest, but is hampered by the low biomass of typical samples. While sample preparation from other microbiomes (e.g., gut) has been the subject of extensive optimization,
Chen, Irene A +3 more
core +1 more source
Phage Genome Annotation Using the RAST Pipeline [PDF]
, 2017 Phages are complex biomolecular machineries that have to survive in a bacterial world. Phage genomes show many adaptations to their lifestyle such as shorter genes, reduced capacity for redundant DNA sequences, and the inclusion of tRNAs in their genomes. In addition, phages are not free-living, they require a host for replication and survival.
Bas E. Dutilh +7 more
openaire +5 more sources
The physicist's guide to one of biotechnology's hottest new topics: CRISPR-Cas
, 2018 Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a multi-functional, constantly evolving immune system in bacteria and archaea cells.
Bonomo, Melia E., Deem, Michael W.
core +1 more source
The natural dietary genistein boosts bacteriophage-mediated cancer cell killing by improving phage-targeted tumor cell transduction [PDF]
, 2016 Gene therapy has long been regarded as a promising treatment for cancer. However, cancer gene therapy is still facing the challenge of targeting gene delivery vectors specifically to tumors when administered via clinically acceptable non-invasive ...
Albahrani, M +5 more
core +2 more sources
Synthesis of Headful Packaging Phages Through Yeast Transformation-Associated Recombination
VirusesDe novo synthesis of phage genomes enables flexible genome modification and simplification. This study explores the synthetic genome assembly of Pseudomonas phage vB_PaeS_SCUT-S4 (S4), a 42,932 bp headful packaging phage, which encapsidates a terminally ...
Cheng Lu +5 more
doaj +1 more source
Dynamics of Bacteriophage Genome Ejection In Vitro and In Vivo
, 2010 Bacteriophages, phages for short, are viruses of bacteria. The majority of phages contain a double-stranded DNA genome packaged in a capsid at a density of ~500 mg/ml. This high density requires substantial compression of the normal B form helix, leading
Molineux, Ian J., Panja, Debabrata
core +1 more source
Modeling the architecture of depolymerase-containing receptor binding proteins in Klebsiella phages [PDF]
, 2019 Klebsiella pneumoniae carries a thick polysaccharide capsule. This highly variable chemical structure plays an important role in its virulence. Many Klebsiella bacteriophages recognize this capsule with a receptor binding protein (RBP) that contains a ...
Briers, Yves +3 more
core +2 more sources
Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97
Viruses, 2015 A novel bacteriophage that infects S. aureus, SA97, was isolated and characterized. The phage SA97 belongs to the Siphoviridae family, and the cell wall teichoic acid (WTA) was found to be a host receptor of the phage SA97.
Yoonjee Chang +5 more
doaj +1 more source
Characterization of bacteriophage communities and CRISPR profiles from dental plaque. [PDF]
, 2014 BackgroundDental plaque is home to a diverse and complex community of bacteria, but has generally been believed to be inhabited by relatively few viruses.
Abeles, Shira R +4 more
core +2 more sources
BMC Microbiology, 2019 Background Pseudomonas aeruginosa is an opportunistic pathogen and one of the leading causes of nosocomial infections. Moreover, the species can cause severe infections in cystic fibrosis patients, in burnt victims and cause disease in domestic animals ...
Anna Cristhina Carmine de Melo +8 more
doaj +1 more source