Results 111 to 120 of about 165,430 (355)
mBio, 2017 During its lysogenic life cycle, the phage genome is integrated into the host chromosome by site-specific recombination. In this report, we analyze lambda phage integration into noncanonical sites using next-generation sequencing and show that it ...
Yu Tanouchi, Markus W. Covert
doaj +1 more source
The physicist's guide to one of biotechnology's hottest new topics: CRISPR-Cas
, 2018 Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a multi-functional, constantly evolving immune system in bacteria and archaea cells.
Bonomo, Melia E., Deem, Michael W.
core +1 more source
Advanced Science, EarlyView.High‐fidelity Nanopore sequencing offers a cost‐effective, portable path to uncovering viral dark matter in complex environments—but suffers from severe read‐length bias, low throughput, and limited accuracy. This study traces these limitations to core biochemical barriers and introduces CLAE, a foundational high‐fidelity platform that enables accurate,
Hannah Yu +14 more
wiley +1 more source
Complete Genome Sequences of Mycobacterium smegmatis Phages NihilNomen and Carlyle, Isolated in Las Vegas, Nevada [PDF]
, 2019 We present the complete genomes of the Mycobacterium smegmatis phages Carlyle and NihilNomen, isolated from soil in Las Vegas, Nevada. The phages were isolated and annotated by undergraduate students enrolled in the Phage Discovery course offered by the ...
Bhatti, Khadija +22 more
core +2 more sources
Advanced Science, EarlyView.This study proposes a novel model illustrating how quorum sensing (QS) regulates the type III‐A CRISPR‐Cas system in Staphylococcus aureus. The QS regulator AgrA suppresses CRISPR‐Cas activity by downregulating the transcriptional regulators SarA and ArcR.
Yang Li +6 more
wiley +1 more source
Network Models of Phage-Bacteria Coevolution [PDF]
, 2006 Bacteria and their bacteriophages are the most abundant, widespread and diverse groups of biological entities on the planet. In an attempt to understand how the interactions between bacteria, virulent phages and temperate phages might affect the ...
Dodd, Ian B. +3 more
core +2 more sources
Advanced Science, EarlyView.Programmable DNA framework captors effectively deplete circulatingtransforming growth factor‐β1 (TGFβ1) through fast systemic clearance and reshape the tumor immune microenvironment, alleviating immunosuppression to inhibit tumor progression. Abstract Efficient modulation of pivotal immune‐regulatory molecules to leverage the tumor microenvironment ...
Xiao Chen +7 more
wiley +1 more source
Engineering Bacteria as Living Therapeutics in Cancer Therapy
Advanced Science, EarlyView.Through synthetic biology approaches, natural bacteria can be genetically programmed into multifunctional living therapeutics. These engineered bacteria are capable of secreting anti‐tumor factors, displaying tumor‐associated antigens on their surface, or undergoing programmed self‐lysis to release therapeutic cargo.
Jiangfeng Chen +6 more
wiley +1 more source
Phage Genome Annotation Using the RAST Pipeline [PDF]
, 2017 Phages are complex biomolecular machineries that have to survive in a bacterial world. Phage genomes show many adaptations to their lifestyle such as shorter genes, reduced capacity for redundant DNA sequences, and the inclusion of tRNAs in their genomes. In addition, phages are not free-living, they require a host for replication and survival.
Bas E. Dutilh +7 more
openaire +5 more sources
Synthesis of Headful Packaging Phages Through Yeast Transformation-Associated Recombination
VirusesDe novo synthesis of phage genomes enables flexible genome modification and simplification. This study explores the synthetic genome assembly of Pseudomonas phage vB_PaeS_SCUT-S4 (S4), a 42,932 bp headful packaging phage, which encapsidates a terminally ...
Cheng Lu +5 more
doaj +1 more source