Results 111 to 120 of about 1,167,436 (302)

PHEIGES: all-cell-free phage synthesis and selection from engineered genomes

Nature Communications
Bacteriophages constitute an invaluable biological reservoir for biotechnology and medicine. The ability to exploit such vast resources is hampered by the lack of methods to rapidly engineer, assemble, package genomes, and select phages.
Antoine Levrier, Ioannis Karpathakis, Bruce Nash, Steven D. Bowden, A. Lindner, V. Noireaux   +5 more
semanticscholar   +1 more source

Engineered Stable, Antibiotic‐Free, High‐Level Protein Expression in the Probiotic Chassis Escherichia coli Nissle 1917

Biotechnology and Bioengineering, EarlyView.
The probiotic E. coli Nissle 1917 was engineered to enable stable and antibiotic free protein expression platforms using native plasmids. Further modification aids in the production of high‐level protein expression for antimicrobial secretion whose activity is comparable to industry standard E. coli BL21.
Halimatun Sakdiah Zainuddin, Sanjeeva Kumar Murali, Thomas J. Mansell   +2 more
wiley   +1 more source

Complex effects of the exo-xis region of the Shiga toxin-converting bacteriophage Φ24B genome on the phage development and the Escherichia coli host physiology [PDF]

, 2023
Sylwia Bloch, Bożena Nejman-Faleńczyk, Katarzyna Licznerska, Aleksandra Dydecka, Gracja Topka, Agnieszka Necel, Alicja Węgrzyn, Grzegorz Węgrzyn   +7 more
openalex   +1 more source

Performance of large virus removal filters during AAV processing: Influence of flux and process disruptions

Biotechnology Progress, EarlyView.
Abstract As adeno‐associated viral vectors (AAV) continue to advance through the clinical pipeline, effective downstream purification strategies must be developed to ensure bulk drug purity and safety. AAV are produced within mammalian cells, bringing forth risks associated with viral contamination.
Akshay S. Chaubal, Asingsa W. Arachchige, Annabelle J. Zahn, S. Ranil Wickramasinghe, Xianghong Qian, Andrew L. Zydney   +5 more
wiley   +1 more source

Mini‐Hairpin DNA: Extraordinarily Stable Structure and Its Applications

Chemistry – A European Journal, EarlyView.
Mini‐hairpin DNA fragments containing GCGAAGC and GCGAAAGC form exceptionally stable hairpin‐like structures, with only two G−C pairs and one sheared G−A pair, remaining undenatured in 7 M urea and resisting nuclease digestion. These mini‐hairpin sequences frequently appear in replication and transcription origins, influence DNA sequencing fidelity ...
Michiko Kimoto, Ichiro Hirao
wiley   +1 more source

Phage Genome Diversity in a Biogas-Producing Microbiome Analyzed by Illumina and Nanopore GridION Sequencing. [PDF]

Microorganisms, 2022
Willenbücher K, Wibberg D, Huang L, Conrady M, Ramm P, Gätcke J, Busche T, Brandt C, Szewzyk U, Schlüter A, Barrero Canosa J, Maus I.   +11 more
europepmc   +1 more source

The Retraction of a Plasmid-Phage Chimera Genome Assembly Study Leaves Serious Issues Unaddressed—Essentially, Nolo Contendere

, 2022
Michael G. Jobling
openalex   +2 more sources

Interpretation of in vitro concentration‐response data for risk assessment and regulatory decision‐making: Report from the 2022 IWGT quantitative analysis expert working group meeting

Environmental and Molecular Mutagenesis, EarlyView.
Abstract Quantitative risk assessments of chemicals are routinely performed using in vivo data from rodents; however, there is growing recognition that non‐animal approaches can be human‐relevant alternatives. There is an urgent need to build confidence in non‐animal alternatives given the international support to reduce the use of animals in toxicity ...
Marc A. Beal, Guangchao Chen, Kerry L. Dearfield, Min Gi, Bhaskar Gollapudi, Robert H. Heflich, Katsuyoshi Horibata, Alexandra S. Long, David P. Lovell, Barbara L. Parsons, Stefan Pfuhler, John Wills, Andreas Zeller, George Johnson, Paul A. White   +14 more
wiley   +1 more source

Genome Sequences of vB_RleM_RL38JI and vB_RleM_RL2RES, Two Virulent Rhizobium leguminosarum Transducing Phages

, 2020
K. M. Damitha Gunathilake, Supriya V. Bhat, Christopher K. Yost, Michael F. Hynes   +3 more
openalex   +1 more source

Predicting promoters in phage genomes using PhagePromoter [PDF]

, 2019
Marta Sampaio, Miguel Rocha, Hugo Oliveira, Óscar Dias   +3 more
openalex   +1 more source