Results 101 to 110 of about 992,387 (299)

An efficient CRISPR-Cas12a tool for iterative genome editing, streamlined minimization, and payload engineering of Pseudomonas phage S1

Synthetic and Systems Biotechnology
The ability to precisely engineer phage genomes is critical for advancing phage-based biotechnology and therapeutic development. Here, we present a high-efficiency, scarless CRISPR-Cas12a genome editing method for a virulent Pseudomonas aeruginosa phage ...
Yanmei Liu, Zizhen Liang, Yanyun Jing, Yanrui Ye, Xiaofeng Yang, Zhanglin Lin   +5 more
doaj   +1 more source

Identification of hepatitis a virus mimotopes by phage display, antigenicity and immunogenicity [PDF]

, 2007
A phage-displayed peptide approach was used to identify ligands mimicking antigenic determinants of hepatitis A virus (HAV) for the first time. Bacteriophages displaying HAV mimotopes were isolated from a phage-display peptide library by affinity ...
Camacho, F., Perez, E.M., Amin, N., Aguilar, A., David I. Stott, Talavera, A., Arturo Talavera, Raiza Martinez, Frank Camacho, Nevis Amin, Ela M. Perez, Larralde, O.G., Alicia Aguilar, Martinez, R., Osmany G. Larralde, Stott, D.I.   +15 more
core   +1 more source

Mesoporous Catalytic‐Adsorptive Nanoregulator Orchestrates Biofilm eDNA/LPS Disassembly and TLR9/TLR4 Immune Reprogramming to Resolve Diabetic Foot Infections

Advanced Science, EarlyView.
Chronic diabetic wounds are hindered by a “dual trap” of biofilm resistance and hyperinflammation. A mesoporous catalytic‐adsorptive nano‐regulator is developed to dismantle this barrier through simultaneous eDNA degradation and LPS sequestration. By silencing TLR4/9‐mediated signaling, the platform clears resilient pathogens and reprograms the immune ...
Junfeng Song, Yang Song, Xirui Huang, Xingjin Li, Lifei Gao, Yixiang Wang, Tianbao Zhu, Xiaomin Li, Shuai Wang, Tiancong Zhao, Dongyuan Zhao   +10 more
wiley   +1 more source

A Novel Dhillonvirus Phage against Escherichia coli Bearing a Unique Gene of Intergeneric Origin

Current Issues in Molecular Biology
Antibiotics resistance is expanding amongst pathogenic bacteria. Phage therapy is a revived concept for targeting bacteria with multiple antibiotics resistances.
Anastasios Vasileiadis, Petros Bozidis, Konstantinos Konstantinidis, Nikolaos Kesesidis, Louiza Potamiti, Anna Kolliopoulou, Apostolos Beloukas, Mihalis I. Panayiotidis, Sophia Havaki, Vassilis G. Gorgoulis, Konstantina Gartzonika, Ioannis Karakasiliotis   +11 more
doaj   +1 more source

Frequent Sweetened Beverage Consumption Is Associated With Accelerated Biological Aging: Evidence From a Population‐Based Study and Gut Microbiota Analysis

Advanced Science, EarlyView.
Frequent sweetened beverage consumption is associated with accelerated biological aging in a large Chinese population. Five gut microbial genera are consistently enriched in both frequent sweetened beverage consumers and individuals with accelerated aging and show significant mediating effects, suggesting a potential microbiota‐related pathway linking ...
Yuwei Shi, Xinmei Li, Yufan Hao, Qiaoyu Wu, Yuji Yu, Siyu Li, Nuo Xu, Yi‐Hsuan Wu, Eunhye Lee, Chi Chang, Emily Hu, Ying Lu, Elizabeth Delzell, Ann W. Hsing, Shankuan Zhu   +14 more
wiley   +1 more source

Genome-scale top-down reduction of phages to generate viable minimal phage genomes

, 2021
Abstract Reduction of tailed-phage genomes to generate viable minimal genome phages is important for expanding our understanding of phage biology, providing insights for phage synthetic biology. Many efforts have been made to minimize living cells, but such work remains a challenge for phages due to the extraordinary genomic diversity and lack ...
Shengjian Yuan, Juan Shi, Jianrong Jiang, Yingfei Ma   +3 more
openaire   +1 more source

Self-assembly of Escherichia coli phage shock protein A

, 2014
The Phage shock protein (Psp) response is an extracytoplasmic stress response. The central component of this system is PspA, a protein that mediates the physiological response to membrane stress.
Male, Abigail, Tavassoli, Ali
core   +1 more source

Systematic Position Mapping of Split CRISPR‐Cas12a Activators Enables Highly Sensitive miRNA Detection and Cancer Cell Stratification

Advanced Science, EarlyView.
ABSTRACT Amplification‐free Cas12a diagnostics with split crRNA enable rapid and programmable target recognition, yet insufficient understanding of DNA activator architecture prevents predictable control over trans‐cleavage activity and sensitivity. Here we systematically map over 200 split DNA activator configurations by introducing nicks at every ...
Xiaoyan Tang, Zhe Li, Yuning Lu, Miao Ma, Zijian Mo, Jiajun Ke, Xinyu Luan, Tiangang Luan, Junqiu Zhai   +8 more
wiley   +1 more source

inPhocus: Current State and Challenges of Phage Research in Singapore.

, 2023
Bacteriophages and phage-derived proteins are a promising class of antibacterial agents that experience a growing worldwide interest. To map ongoing phage research in Singapore and neighboring countries, Lee Kong Chian School of Medicine, Nanyang ...
Kwa, AL-H, Hariharan, S, Chung, SJ, Goh, BC, Paul, VD, Verma, NK, Ostrowski, A, Lakshminarayanan, R, Sriram, B, Chen, J, Clokie, MRJ, Sicheritz-Pontén, T, Rice, SA, Dam, LC, Chan, CC, Goh, KCM, Aung, TT, Chen, H, Bifani, P, Razali, H, Gautam, HK, Thong, SH, Ismail, MH, Bhattacharyya, MS, van Steensel, MAM, Low, SWY, Tan, SJ, Ho, P, Moreira, W   +28 more
core   +1 more source

A Comprehensive Microbial Gene Catalog of the Human Airway Microbiome Across Anatomical Sites and Geographic Regions

Advanced Science, EarlyView.
An integrated Human Airway Microbiome Gene Catalog (iHAMGC) is constructed from 12,273 metagenomic airway samples, providing a high‐resolution reference of respiratory microbial genes. The catalog enables systematic exploration of taxonomic and functional variation across airway niches and geographic regions, revealing site‐specific and region ...
Qing Zhang, Dashu Li, Bo Liu, Yue Zhang, Min Li, Ruochun Guo, Yawen Ni, Shibin Chen, Biao Ni, Lijie Qiu, Guorui Xing, He Dong, Qiulong Yan, Shenghui Li, Xiaohui Zou, Bin Cao   +15 more
wiley   +1 more source