Results 71 to 80 of about 2,642,573 (281)

Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo

Advanced Science, EarlyView.
As a compact type II‐D system, the Cas9d‐based platform holds great potential for in vivo applications. Through rational engineering, its derived base editors (9dBEs) enable efficient disease modeling while facilitating single‐vector AAV delivery for in vivo genome editing. These miniature tools offer a robust strategy for basic research and biomedical
Qingquan Xiao, Zhijin Tian, Luqi Weng, Kun Xu, Dingyi Han, Zhaowei Wu, Junnan Li, Shangpu Li, Qijing Zhang, Xiaohui Zhao, Guoling Li, Yinghui Wei, Zhen Gu   +12 more
wiley   +1 more source

Streptococcal Mannose Phosphotransferase System Component IID Is a Novel RANK‐Binding Osteoclastogenic Factor

Advanced Science, EarlyView.
Streptococcal mannose phosphotransferase system component IID (Man‐PTSIID) is identified as a novel RANK‐binding osteoclastogenic factor. By directly binding to RANK and activating NF‐κB independently of TLR2, Man‐PTSIID drives osteoclastogenesis and inflammatory bone destruction, uncovering an unexpected microbial mechanism underlying streptococcal ...
Chaeyeon Park, Yeongkag Kwon, Yeonjin Lim, Jeong Woo Park, Hyun Jung Ji, Dongwook Lee, Jueun Lee, Sung‐Ho Yun, Sungho Jeong, Ho Seong Seo, Kun Cho, Cheol‐Heui Yun, Ok‐Jin Park, Seung Hyun Han   +13 more
wiley   +1 more source

Complete Pathway Refactoring and Combinatorial Biosynthesis of Anthracyclines for Modulation of Histone Eviction and DNA Damage Bioactivities

Angewandte Chemie, EarlyView.
A synthetic biology platform was established for the complete refactoring of complex anthracycline biosynthetic pathways in Streptomyces coelicolor using standardized, chemically synthetic DNA parts. This modular construction enabled streamlined combinatorial biosynthesis, yielding novel analogs that decouple DNA damage from chromatin damage (histone ...
Rongbin Wang, Heli Tirkkonen, Sabina Y. van der Zanden, Larissa Ponomareva, Isaline Fagon, Leslie Fève, Kendall Paige, Courtney L. Brown, Nora Schwartz, Jacob Hecht, Jennifer Nguyen, Jon S. Thorson, Jacques Neefjes, S. Eric Nybo, Khaled A. Shaaban, Mikko Metsä‐Ketelä   +15 more
wiley   +2 more sources

Extensive library of Ruminococcus gnavus phages revealing genomic and phenotypic diversity with novel clusters

Microbiology Spectrum
Ruminococcus gnavus is a prevalent member of human gut microbiota and is associated with inflammatory bowel disease. Phage-based microbiota engineering represents a promising approach for alleviating dysbiosis; however, knowledge regarding R.
Arata Sakiyama, Ayaka Washizaki, Keiko Inaba-Hasegawa, Most Hushna Ara Naznin, Keigo Ide, Masayuki Takase, Yoriko Ibuki, Yuichi Sawaguchi, Shoichi Mitsunaka, Yutaro Ono, Islam Mohammad Shyful, Hiroki Ando   +11 more
doaj   +1 more source

Efficacy of systemic temozolomide‐activated phage‐targeted gene therapy in human glioblastoma

EMBO Molecular Medicine, 2019
Glioblastoma multiforme (GBM) is the most lethal primary intracranial malignant neoplasm in adults and most resistant to treatment. Integration of gene therapy and chemotherapy, chemovirotherapy, has the potential to improve treatment. We have introduced
Justyna Magdalena Przystal, Sajee Waramit, Md Zahidul Islam Pranjol, Wenqing Yan, Grace Chu, Aitthiphon Chongchai, Gargi Samarth, Nagore Gene Olaciregui, Ghazaleh Tabatabai, Angel Montero Carcaboso, Eric Ofori Aboagye, Keittisak Suwan, Amin Hajitou   +12 more
doaj   +1 more source

Bacteriophage-based nano-biosensors for the fast impedimetric determination of pathogens in food samples

Scientific Reports, 2023
The early and rapid detection of pathogenic microorganisms is of critical importance in addressing serious public health issues. Here, a new bacteriophage-based nano-biosensor was constructed and the electrochemical impedimetric method was fully ...
Nader Abdelhamied, Fatma Abdelrahman, Ayman El-Shibiny, Rabeay Y. A. Hassan   +3 more
doaj   +1 more source

One‐Step Immobilized Phage Depolymerase Coatings for Catheter‐Related Bloodstream Infections

Advanced Science, EarlyView.
Catheter‐related bloodstream infections (CRBSI) caused by drug‐resistant Gram‐negative bacteria remain a major clinical challenge. Here, a polydopamine‐assisted depolymerase coating degrades bacterial capsules and restores serum‐mediated killing, effectively suppressing biofilms and reducing bacterial burden in vitro, ex vivo, and in vivo, presenting a
Lingkai Dong, Xingjin Li, Tao Hu, Qianqian Lu, Xiaoxu Zhang, Xi Chen, Yifan Luo, Zhong Zuo, Ronald Man Yeung Wong, Miao Xu, Tiancong Zhao, Sharon Shui Yee Leung   +11 more
wiley   +1 more source

Stabilizing Functional Nucleic Acids for Real‐Sample Diagnostics

Angewandte Chemie, EarlyView.
Functional nucleic acids enable programmable real‐sample diagnostics, but often lose function in complex biological matrices. This review highlights four stabilization levers—chemical, topological, architectural, and microenvironment/interface engineering—that preserve aptamer and DNAzyme performance in saliva, serum, urine, and stool, reframing ...
Soyeon Lee, Letizia Dondi, Amal Mathai, Connor Nurmi, Yingfu Li   +4 more
wiley   +2 more sources

Efficacy of phage vB_EcoP_ZCEC16 in controlling Escherichia coli contamination in fresh poultry meat

BMC Microbiology
Avian pathogenic Escherichia coli (APEC) poses a substantial economic and health challenges for poultry and meat industries. One of the promising alternative approaches to overcome bacterial contamination in food products is bacteriophage, due to its ...
Azza G. Kamel, Zainab K. Hammouda, Sohaila Tarek, Yousef Nassar, Eman E. Elawsya, Ahmed Osama, Bishoy Maher Zaki, Mohammad Gharaibeh, Rashad Al-Hindi, Ihab Habib, Ayman El-Shibiny   +10 more
doaj   +1 more source

A Synthetic Platform for Antibody Junctional Diversification Beyond Natural Constraints

Advanced Science, EarlyView.
ARESEC is a synthetic platform that reconstructs functional V(D)J recombination in HEK293T cells. By introducing RSS elements and co‐expressing RAG1/2 and TdT across all three antibody CDRs, it enables programmable junctional diversification and direct generation of high‐affinity variants targeting PD‐1/PD‐L1, overcoming the evolutionary constraints of
Wang Liu, Jun Yang, Yuhang Zhang, Xiaoli Xue   +3 more
wiley   +1 more source