Results 1 to 10 of about 340,700 (262)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Crystal structures and snapshots along Tpt1-catalyzed phosphate transfer from nucleic acid to NAD+

Nature Communications
Tpt1/TRPT1/KptA family proteins are evolutionarily conserved in all three domains of life. In fungi and plants, Tpt1 transfers 2’-PO4 2- from tRNA splice junction to NAD+, which is the final step of tRNA maturation and is critical for the function of ...
Chulei Cao, Jie Yang, Weizhen Zhang, Jiaqin Chen, Yanqing Gao, Yuqi Yao, Yixi Zhang, Huili Li, Linxi Li, Zhaorong Luo, Chenxi Wang, Guangli Tang, Ruixue Cui, Hehua Liu, Qiyao Wang, Zhen Huang, Jinbiao Ma, Jianhua Gan   +17 more
doaj   +1 more source

Laryngeal symptoms related to motor phenotypes in Parkinson's disease: A systematic review

Laryngoscope Investigative Otolaryngology, 2023
Objective This study aimed to systematically review the associations between motor clinical phenotypes in Parkinson's disease (PD) and laryngeal disease symptoms.
Zoe Thijs, Matthew Dumican
doaj   +1 more source

The Integrated Phenotype [PDF]

Integrative and Comparative Biology, 2012
Proper functioning of complex phenotypes requires that multiple traits work together. Examination of relationships among traits within and between complex characters and how they interact to function as a whole organism is critical to advancing our understanding of evolutionary developmental plasticity.
openaire   +2 more sources

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

Comprehensive genomic and transcriptomic analyses reveal prognostic stratification for esophageal squamous cell carcinoma

Signal Transduction and Targeted Therapy
Recent large-scale multi-omics studies have characterized the heterogeneity of esophageal squamous cell carcinoma (ESCC), but inconsistent clinical management has hindered the identification of prognostic markers and patient stratification.
Jian Gao, Qiming Wang, Fangqiu Fu, Yue Zhao, Teng Yang, Xiangze Li, Yihua Sun, Hong Hu, Longfei Ma, Longsheng Miao, Xiaoyang Luo, Ting Ye, Yiliang Zhang, Yang Zhang, Ziling Huang, Hang Li, Longlong Shao, Midie Xu, Kuaile Zhao, Shiyue Zhang, Mou Zhang, Jun Wang, Chong Dai, Xiaoxiao Shang, Tingyi An, Yawei Zhang, Jiaqing Xiang, Zhiwei Cao, Bin Li, Haiquan Chen   +29 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

DNA Subunit Vaccine and Recombinant BCG Based on Mycobacterial Lipoprotein LprO Enhance Anti-Tuberculosis Protection in the Lungs of Mice

Vaccines
Background/Objectives: Over the past two centuries, tuberculosis (TB) has been responsible for approximately one billion deaths and continues to represent a significant global health challenge.
Weili Huang, Shuqin Xu, Lifang Shen, Dan Chen, Hanmei Liu, Yuting Tang, Xiaolin Liu, Wenxuan Xiao, Ziwei Zhou, Shifeng Zhang, Jixi Li, Xiaoyong Fan, Yuefeng Chu, Lu Zhang   +13 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source