Cloning and expression of phosphoenolpyruvate carboxykinase from Escherichia coli
Journal of Hebei University of Science and Technology, 2019 In order to better study the structure and function of phosphoenolpyruvate carboxylase, the phosphoenolpyruvate carboxylase gene is cloned and expressed by PCR (polymerase chain reaction), double enzyme digestion and cell transformation. The results show
Xiaoliang HE +6 more
doaj +1 more source
Repression of glucocorticoid-stimulated angiopoietin-like 4 gene transcription by insulin. [PDF]
, 2014 Angiopoietin-like 4 (Angptl4) is a glucocorticoid receptor (GR) primary target gene in hepatocytes and adipocytes. It encodes a secreted protein that inhibits extracellular LPL and promotes adipocyte lipolysis.
Chen, Tzu-Chieh +6 more
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Cancer Research, 2015 Although metabolic defects have been investigated extensively in differentiated tumor cells, much less attention has been directed to the metabolic properties of stem-like cells that repopulate tumors [tumor-repopulating cells (TRC)].
Yong Li +13 more
semanticscholar +1 more source
Minimizing acetate formation in E. coli fermentations [PDF]
, 2007 Escherichia coli remains the best established production organisms in industrial biotechnology. However, during aerobic fermentation runs at high growth rates, considerable amounts of acetate are accumulated as by-product.
De Maeseneire, Sofie +3 more
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A Reactive Cysteine in Avian Liver Phosphoenolpyruvate Carboxykinase [PDF]
Journal of Biological Chemistry, 1989 The modification of avian phosphoenolpyruvate carboxykinase by a variety of sulfhydryl reagents leads to inhibition. The inhibition is related to the loss of 1 highly reactive cysteine residue of the 13 cysteines present in the enzyme. Inhibition by reagents which yield a mixed disulfide was rapidly reversed by thiols.
A L Makinen, Thomas Nowak
openaire +2 more sources
Lack of association between PCK1 polymorphisms and obesity, physical activity, and fitness in European Youth Heart Study (EYHS) [PDF]
, 2010 Phosphoenolpyruvate carboxykinase-1 (PCK1) is the rate-limiting enzyme in the hepatic gluconeogenic pathway. Studies have shown that overexpression of Pck1 in mice results in obesity-related traits and higher levels of physical activity (PA).
Anders Grontved +45 more
core +1 more source
Journal of Industrial Microbiology & Biotechnology, 2015 Aspergillus carbonarius has a potential as a cell factory for production of various organic acids. In this study, the organic acid profile of A. carbonarius was investigated under different cultivation conditions.
Lei Yang, M. Lübeck, P. S. Lübeck
semanticscholar +1 more source
Changes in the microsomal proteome of tomato fruit during ripening [PDF]
, 2019 The variations in the membrane proteome of tomato fruit pericarp during ripening have been investigated by mass spectrometry-based label-free proteomics.
De Lorenzo, Giulia +6 more
core +1 more source
Association of PCK1 with Body Mass Index and Other Metabolic Features in Patients With Psychotropic Treatments. [PDF]
, 2015 Weight gain is a major health problem among psychiatric populations. It implicates several receptors and hormones involved in energy balance and metabolism.
Aubry, J.M. +15 more
core +2 more sources
Elucidating fish oil-induced milk fat depression in dairy sheep: Milk somatic cell transcriptome analysis [PDF]
, 2017 9 páginas, 4 figuras.In this study, RNA sequencing was used to obtain a comprehensive profile of the transcriptomic changes occurring in the mammary gland of lactating sheep suffering from fish oil-induced milk fat depression (FO-MFD).
Arranz, Juan José +5 more
core +1 more source