Results 41 to 50 of about 41,991 (249)

Extraction and Measurement the Activities of Cytosolic Phosphoenolpyruvate Carboxykinase (PEPCK) and Plastidic NADP-dependent Malic Enzyme (ME) on Tomato (Solanum lycopersicum)

Bio-Protocol, 2014
A recent study demonstrated that cytosolic phosphoenolpyruvate carboxykinase (PEPCK) and NADP-malic enzyme (NADP-ME) have an important role in malate metabolism during fruit ripening (Osorio et al., 2013). PEPCK catalyze the ATP-dependent decarboxylation
Sonia Osorio, José Vallarino, Marek Szecowka, Shai Ufaz, Vered Tzin, Ruthie Angelovici, Gad Galili, Fayezeh Aarabi   +7 more
doaj   +1 more source

Promoter Polymorphism in PCK1 (Phosphoenolpyruvate Carboxykinase Gene) Associated with Type 2 Diabetes Mellitus [PDF]

, 2004
"We sequenced the promoter and coding regions of PCK1 encoding cytosolic phosphoenolpyruvate carboxykinase from genomic DNA of subjects with type 2 diabetes mellitus (DM).
Hanley, Anthony J.G., Harris, S. B., Hegele, Robert A., Young, T. Kue, van der Veer, Eric, Ban, Matthew R., Zinman, Bernard, Pickering, J. Geoffrey, Cao, Henian   +8 more
core   +1 more source

Comprehensive Analysis of C. glutamicum Anaplerotic Deletion Mutants Under Defined d-Glucose Conditions

Frontiers in Bioengineering and Biotechnology, 2021
Wild-type C. glutamicum ATCC 13032 is known to possess two enzymes with anaplerotic (C4-directed) carboxylation activity, namely phosphoenolpyruvate carboxylase (PEPCx) and pyruvate carboxylase (PCx). On the other hand, C3-directed decarboxylation can be
Jannick Kappelmann, Bianca Klein, Mathias Papenfuß, Julian Lange, Bastian Blombach, Ralf Takors, Wolfgang Wiechert, Tino Polen, Stephan Noack   +8 more
doaj   +1 more source

Alteration of de novo glucose production contributes to fasting hypoglycaemia in fyn deficient mice [PDF]

, 2013
Previous studies have demonstrated that glucose disposal is increased in the Fyn knockout (FynKO) mice due to increased insulin sensitivity. FynKO mice also display fasting hypoglycaemia despite decreased insulin levels, which suggested that hepatic ...
Bastie, Claire C., Kurland, Irwin J., Elena Tarabra, Gustavo Palacios, Jeffrey E Pessin, Yang Yingjuan, Bastie Claire C., Tarabra, Elena, Gong-She Yang (490794), Claire C. Bastie (187271), Palacios Gustavo, Yingjuan Yang (490792), Irwin J. Kurland (177409), Claire C Bastie, Yingjuan Yang, Yang Gong-She, Yang, Gong-She, Irwin J Kurland, Vaitheesvaran, Bhavapriya, Palacios, Gustavo, Pessin Jeffrey E., Jeffrey E. Pessin (187274), Vaitheesvaran Bhavapriya, Yang, Yingjuan, Bhavapriya Vaitheesvaran (177385), Elena Tarabra (490793), Tarabra Elena, Bhavapriya Vaitheesvaran, Gustavo Palacios (67191), Kurland Irwin J., Gong-She Yang, Pessin, Jeffrey E.   +31 more
core   +2 more sources

Cloning and expression of phosphoenolpyruvate carboxykinase from Escherichia coli

Journal of Hebei University of Science and Technology, 2019
In order to better study the structure and function of phosphoenolpyruvate carboxylase, the phosphoenolpyruvate carboxylase gene is cloned and expressed by PCR (polymerase chain reaction), double enzyme digestion and cell transformation. The results show
Xiaoliang HE, Zhu2 LI, Liqiang2 LIANG, Wanli MENG, Lantian MIAO, Yaozu SUN, Hongguang2 CAI   +6 more
doaj   +1 more source

Phosphoenolpyruvate Carboxykinase Activity in Grape Berries [PDF]

Plant Physiology, 1975
Phosphoenolpyruvate (PEP) carboxykinase activity was found in crude extracts of ;Pinot noir' grape berries. The enzyme required ATP, Mn(2+) plus Mg(2+), a pH of 6.6, and a temperature of 40 C for maximum activity. The range in concentration of oxaloacetic acid needed for maximum phosphoenolpyruvate carboxykinase activity was 5 to 10 mm, and the Km for ...
H P, Ruffner, W M, Kliewer
openaire   +2 more sources

Conservation from rat to human of cytosolic phosphoenolpyruvate carboxykinase and the control of its gene expression [PDF]

, 1985
Structural conservation of cytosolic phosphoenolpyruvate carboxykinase protein and mRNA sequence was found in all species examined from rodents to human. The mitochondrial isoenzyme, in all species tested, represents a distinct protein.
Oded Meyuhas, David Mencher, Nissim Benvenisty, Cohen, Hannah, Hannah Cohen, Shouval, Daniel, Bruriah Gidoni, Lea Reshef, Gidoni, Bruriah, Reshef, Lea, Mencher, David, Daniel Shouval, Benvenisty, Nissim, Meyuhas, Oded   +13 more
core   +1 more source

Effect of pyruvate kinase gene deletion on the physiology of Corynebacterium glutamicum ATCC13032 under biotin-sufficient non-glutamate-producing conditions: Enhanced biomass production

Metabolic Engineering Communications, 2015
The effect of pyruvate kinase gene (pyk) deletion on the physiology of Corynebacterium glutamicum ATCC13032 was investigated under biotin-sufficient, non-glutamate-producing conditions.
Kazunori Sawada, Masaru Wada, Takuya Hagiwara, Susumu Zen-in, Keita Imai, Atsushi Yokota   +5 more
doaj   +1 more source

Inhibition of Pig Phosphoenolpyruvate Carboxykinase Isoenzymes by 3-Mercaptopicolinic Acid and Novel Inhibitors. [PDF]

PLoS ONE, 2016
There exist two isoforms of cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C) in pig populations that differ in a single amino acid (Met139Leu). The isoenzymes have different kinetic properties, affecting more strongly the Km and Vmax of nucleotides.
Jorge Hidalgo, Pedro Latorre, José Alberto Carrodeguas, Adrián Velázquez-Campoy, Javier Sancho, Pascual López-Buesa   +5 more
doaj   +1 more source

A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase [PDF]

, 2008
To determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium.
Kim, Pil, Lee, Sang Yup, Kwon, Yeong-Deok   +2 more
core   +1 more source