Endocrinology and Metabolism, 2012 배경: 세포 내 신호전달에 중요한 단백질인 포스포리파제 C-β4(phospholipase C-β4, PLC-β4)의 물리화학적 성질을 연구하기 위해서는 다량의 정제된 단백질이 필요하나 기존의 망막 조직을 이용한 정제 방법은 그 방법이 매우 어려워 충분한 양의 단백질을 정제하는 것이 거의 불가능하다. 따라서 이를 쉽게 정제하기 위하여 배큘로바이러스(baculovirus)를 이용한 발현 및 정제 방법을 개발할 필요가 있다. 또한 배큘로바이러스를 이용한 정제 방법이 확립된 경우 이를 이용하여 키메라 단백질의 발현도 쉽게 할 수 있어 단백질의 성질연구에 매우 유용하다.
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Suramin inhibits the early effects of PLA(2) neurotoxins at mouse neuromuscular junctions: a twitch tension study [PDF]
, 2011 Several phospholipase A(2) (PLA(2)) neurotoxins from snake venoms can affect acetylcholine release at the neuromuscular junction. In isolated nerve-muscle preparations three distinct phases have been described for this phenomenon: An initial transient ...
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Phospholipase A2 and phospholipase C are activated by distinct GTP-binding proteins in response to alpha 1-adrenergic stimulation in FRTL5 thyroid cells. [PDF]
, 1986 Ronald M. Burch +2 more
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Journal of Lipid Research, 2000 Initiation of atherosclerosis is characterized by accumulation of aggregates of small lipid droplets and vesicles in the extracellular matrix of the arterial intima.
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Influence of phospholipase C on some electrical properties of the skeletal muscle membrane
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Enzymatic Hydrolysis of Sphingosylphosphorylcholine by Phospholipase C
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Recognition by cytotoxic T lymphocytes of Qa-2 antigens. Sensitivity of Qa-2 molecules to phosphatidylinositol-specific phospholipase C. [PDF]
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Activation Mechanisms and Diverse Functions of Mammalian Phospholipase C. [PDF]
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