Results 221 to 230 of about 19,927,495 (255)
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A simplified assay for phospholipase C
Analytical Biochemistry, 1979Abstract A new assay for phospholipase C activity that uses alkaline phosphatase to convert phosphorylcholine to inorganic phosphate is described. The determination of inorganic phosphate is performed in the presence of phosphatidylcholine and protein after the addition of sodium dodecyl sulfate.
Nancy J. Truesdale +2 more
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Aspirin inhibits phospholipase C
Biochemical and Biophysical Research Communications, 1986We have shown previously that aspirin (ASA) ingestion by normal human volunteers inhibits peripheral blood monocyte phospholipase C (PLC) activities ex vivo. In order to explore further the mechanism of action of ASA, normal human monocytes and differentiated human U937 cells were treated with ASA and other salicylates. Cells preincubated with ASA were
Mike A. Clark +2 more
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2003
Publisher Summary This chapter provides an overview of mammalian phospholipase C. The hydrolysis of cellular phospholipids leads to the formation of various bioactive lipid mediators, acting either as extracellular signaling molecules or as intracellular second messengers. A well-known second messenger-forming system involving phospholipid hydrolysis
Martina Schmidt +4 more
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Publisher Summary This chapter provides an overview of mammalian phospholipase C. The hydrolysis of cellular phospholipids leads to the formation of various bioactive lipid mediators, acting either as extracellular signaling molecules or as intracellular second messengers. A well-known second messenger-forming system involving phospholipid hydrolysis
Martina Schmidt +4 more
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Purification of Phospholipase C β and Phospholipase C ε from Sf9 Cells
2003Phosphatidylinositol-specific phospholipase C (PLC) enzymes catalyze hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) generating diacylglycerol (DAG) and inositol 1,4,5-triphosphate (IP(3)). The PLC beta isoforms of PLCs are activated by G proteins after hormone or neurotransmitter stimulation of G protein-coupled receptors (GPCR).
Huan Wang +3 more
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Increase of the catalytic activity of phospholipase C-gamma 1 by tyrosine phosphorylation.
Science, 1990Phospholipase C-gamma 1 (PLC-gamma 1), an isozyme of the phosphoinositide-specific phospholipase C family, which occupies a central role in hormonal signal transduction pathways, is an excellent substrate for the epidermal growth factor (EGF) receptor ...
S. Nishibe +5 more
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[22] Bioluminescent assay for lipase, phospholipase A2, and phospholipase C
1981Publisher Summary This chapter describes the bioluminescent assay for lipase, phospholipase A 2 , and phospholipase C. The dim mutant M17 of the marine luminous bacterium Beneckea harveyi MAV #392 emits light with either exogenous aldehydes or fatty acids.
Michael Heller, Shimon Ulitzur
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Protein kinase C‐dependent stimulation of phospholipase D in phospholipase C‐treated fibroblasts
Lipids, 1993AbstractTreatment of [14C]choline‐ or [14C]ethanolamine‐labeled NIH 3T3 fibroblasts withBacillus cereus phosphatidylcholine‐specific phospholipase C (PLC) enhanced phospholipase D (PLD)‐mediated hydrolysis of the respective14C‐labeled phospholipids. PLD activity was stimulated by 1.5 U/mL of POLC and by 100 nM of the protein kinase C (PKC) activator ...
Zoltan Kiss, Nandor Garamszegi
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[49] Properties of phospholipase C isozymes
1991Publisher Summary Phospholipase C (PLC)-catalyzed hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP 2 ) has been proposed to be a crucial step in the cellular response to calcium-mobilizing hormones and neurotransmitters. Numerous attempts to isolate PLC from various tissues have been made and as a result, various kinds of PLC have been ...
Yasufumi Emori +2 more
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Chemistry and Physics of Lipids, 1999
Activation of PLC-gamma isozymes in response to various agonists involves tyrosine phosphorylation of the effector enzymes. Recent evidence indicates that PLC-gamma isozymes are additionally activated by phosphatidic acid, phosphatidylinositol 3,4,5-trisphosphate and arachidonic acid in the absence of PLC-gamma tyrosine phosphorylation.
Yun Soo Bae, Sue Goo Rhee, Fujio Sekiya
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Activation of PLC-gamma isozymes in response to various agonists involves tyrosine phosphorylation of the effector enzymes. Recent evidence indicates that PLC-gamma isozymes are additionally activated by phosphatidic acid, phosphatidylinositol 3,4,5-trisphosphate and arachidonic acid in the absence of PLC-gamma tyrosine phosphorylation.
Yun Soo Bae, Sue Goo Rhee, Fujio Sekiya
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Phospholipase C- 1: A Phospholipase and Guanine Nucleotide Exchange Factor
Molecular Interventions, 2002Although phospholipase C-gamma (PLC-gamma) participates in cellular mitogenesis, evidence indicates that the catalytic activity of PLC-gamma (to hydrolyze certain phosphoinositides) is nonessential to the process. So how is it that PLC-gamma is necessary but its lipase activity is not?
Michael Moran, Zhixiang Wang
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