Phospholipase D3 (PLD3) is a protein of unclear function that structurally resembles other members of the phospholipase D superfamily. A coding variant in this gene confers increased risk for the development of Alzheimer's disease (AD), although the ...
Alex G Nackenoff+12 more
doaj +1 more source
Regulation of phospholipase D [PDF]
Structural studies of plant and bacterial members of the phospholipase D (PLD) superfamily are providing information about the role of the conserved HKD domains in the structure of the catalytic center and the catalytic mechanism of mammalian PLD isozymes (PLD1 and PLD2).
openaire +4 more sources
Influence of Lipid Heterogeneity and Phase Behavior on Phospholipase A2 Action at the Single Molecule Level [PDF]
We monitored the action of phospholipase A2 (PLA2) on L- and D-dipalmitoylphosphatidylcholine (DPPC) Langmuir monolayers by mounting a Langmuir-trough on a wide-field fluorescence microscope with single molecule sensitivity. This made it possible to directly visualize the activity and diffusion behavior of single PLA2 molecules in a heterogeneous ...
arxiv +1 more source
Phospholipase D family member 4, a transmembrane glycoprotein with no phospholipase D activity, expression in spleen and early postnatal microglia. [PDF]
BackgroundPhospholipase D (PLD) catalyzes conversion of phosphatidylcholine into choline and phosphatidic acid, leading to a variety of intracellular signal transduction events.
Fumio Yoshikawa+14 more
doaj +1 more source
Crystal Structures of the Plant Phospholipase A1 Proteins Reveal a Unique Dimerization Domain
Phospholipase is an enzyme that hydrolyzes various phospholipid substrates at specific ester bonds and plays important roles such as membrane remodeling, as digestive enzymes, and the regulation of cellular mechanism.
Yunseok Heo+8 more
doaj +1 more source
Detection of virulence factors and beta-lactamase encoding genes among the clinical isolates of Pseudomonas aeruginosa [PDF]
Background: Pseudomonas aeruginosa has emerged as a significant opportunistic bacterial pathogen that causes nosocomial infections in healthcare settings resulting in treatment failure throughout the world. This study was carried out to compare the relatedness between virulence characteristics and \b{eta}-lactamase encoding genes producing Pseudomonas ...
arxiv +1 more source
On the Complexity of Distance-$d$ Independent Set Reconfiguration [PDF]
For a fixed positive integer $d \geq 2$, a distance-$d$ independent set (D$d$IS) of a graph is a vertex subset whose distance between any two members is at least $d$. Imagine that there is a token placed on each member of a D$d$IS. Two D$d$ISs are adjacent under Token Sliding ($\mathsf{TS}$) if one can be obtained from the other by moving a token from ...
arxiv +1 more source
Soybean phospholipase D activity determination. A comparison of two methods
Due to a discrepancy between previously published results, two methods to determine the soybean phospholipase D activity were evaluated. One method is based on the extraction of the enzyme from whole soybean flour, quantifying the enzyme activity on the ...
E. Tosi, G. Ballerini, E. Ré
doaj +1 more source
Transphosphatidylation by Phospholipase D
Abstract Transphosphatidylase activity was recognized in several plant tissues as well as in extracts. It was attributed to phospholipase D. This enzyme was purified 110-fold from Savoy cabbage. The ratio of its hydrolase to its transphosphatidylase activity remained constant throughout the purification. Additional evidence supports the conclusion that
Sofia M. Freer+2 more
openaire +2 more sources
Phospholipase D in the Golgi apparatus [PDF]
Phospholipase D has long been implicated in vesicle formation and vesicular transport through the secretory pathway. The Golgi apparatus has been shown to exhibit a plethora of mechanisms of vesicle formation at different stages to accommodate a wide variety of cargo.
Dennis Shields+2 more
openaire +3 more sources