Protocol to assess substrate dephosphorylation by serine/threonine phosphoprotein phosphatases in vitro [PDF]
STAR Protocols, 2023 Summary: Serine/threonine protein phosphatase 2 (PP2A) forms heterotrimeric holoenzymes, where a scaffold subunit bridges the PP2A catalytic subunit to a B regulatory subunit, e.g., B55α.
Jason S. Wasserman +6 more
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Unfair competition governs the interaction of pCPI-17 with myosin phosphatase (PP1-MYPT1) [PDF]
eLife, 2017 The small phosphoprotein pCPI-17 inhibits myosin light-chain phosphatase (MLCP). Current models postulate that during muscle relaxation, phosphatases other than MLCP dephosphorylate and inactivate pCPI-17 to restore MLCP activity.
Joshua J Filter +4 more
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Molecular evolution of phosphoprotein phosphatases in Drosophila. [PDF]
PLoS ONE, 2011 Phosphoprotein phosphatases (PPP), these ancient and important regulatory enzymes are present in all eukaryotic organisms. Based on the genome sequences of 12 Drosophila species we traced the evolution of the PPP catalytic subunits and noted a ...
Márton Miskei +4 more
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The PP2A/4/6 subfamily of phosphoprotein phosphatases regulates DAF-16 and confers resistance to environmental stress in postreproductive adult C. elegans. [PDF]
PLoS ONE, 2020 Insulin and insulin-like growth factors are longevity determinants that negatively regulate Forkhead box class O (FoxO) transcription factors. In C. elegans mutations that constitutively activate DAF-16, the ortholog of mammalian FoxO3a, extend lifespan ...
Rebecca S Rivard +2 more
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Coordination of Protein Kinase and Phosphoprotein Phosphatase Activities in Mitosis [PDF]
Frontiers in Cell and Developmental Biology, 2018 Dynamic changes in protein phosphorylation govern the transitions between different phases of the cell division cycle. A “tug of war” between highly conserved protein kinases and the family of phosphoprotein phosphatases (PPP) establishes the ...
Isha Nasa +3 more
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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors. [PDF]
J Vis Exp, 2022 Smolen KA, Kettenbach AN.
europepmc +3 more sources
Affinity-based profiling of endogenous phosphoprotein phosphatases by mass spectrometry. [PDF]
Nat Protoc, 2021 Brauer BL +5 more
europepmc +3 more sources
Substrate and phosphorylation site selection by phosphoprotein phosphatases. [PDF]
Trends Biochem Sci, 2023 Nguyen H, Kettenbach AN.
europepmc +3 more sources
PHOSPHORYLATION/DEPHOSPHORYLATION OF MITOCHONDRIAL PROTEINS IN REDOX-SIGNALLING OF HIGHER PLANTS UNDER ABIOTIC STRESS CONDITIONS [PDF]
Journal of Stress Physiology & Biochemistry, 2012 We studied an impact of the widely spread intra-cellular signals Ca2+ and сAMP on activity of the protein phosphorylation in maize mitochondria. The use of the isolated mitochondria is a convenient model system for investigation of the different ...
Subota I.Yu. +4 more
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BBA Advances, 2021 Background: Phosphoprotein phosphatases (PPP) belong to the PPP Sequence family, which in turn belongs to the broader metallophosphoesterase (MPE) superfamily.
David Kerk +5 more
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