An Affinity-directed Protein Missile (AdPROM) System for Targeted Destruction of Endogenous Proteins
Bio-Protocol, 2017 We recently reported an Affinity-directed PROtein Missile (AdPROM) system for the targeted proteolysis of endogenous proteins of interest (POI) (Fulcher et al., 2016 and 2017).
Thomas Macartney +2 more
doaj +1 more source
Phosphorylation is required for the pathogen defense function of the Arabidopsis PEN3 ABC transporter. [PDF]
, 2017 The Arabidopsis PEN3 ABC transporter accumulates at sites of pathogen detection, where it is involved in defense against a number of pathogens. Perception of PAMPs by pattern recognition receptors initiates recruitment of PEN3 and also leads to PEN3 ...
Somerville, Shauna, Underwood, William
core +1 more source
Mapping and Functional Role of Phosphorylation Sites in the Thyroid Transcription Factor-1 (TTF-1) [PDF]
, 1996 The phosphorylation of thyroid transcription factor-1 (TTF-1), a homeodomain-containing transcription factor that is required for thyroid-specific expression of the thyroglobulin and thyroperoxidase gene promoters, has been studied.
Acebron, Alvaro +6 more
core +1 more source
Proteolysis of Non-phosphorylated and Phosphorylated Tau by Thrombin [PDF]
Journal of Biological Chemistry, 2005 The microtubule-associated protein tau aggregates intracellularly by unknown mechanisms in Alzheimer's disease and other tauopathies. A contributing factor may be a failure to break down free cytosolic tau, thus creating a surplus for aggregation, although the proteases that degrade tau in brain remain unknown.
Patrick L. McGeer +2 more
openaire +3 more sources
Determination of VPS34/PIK3C3 Activity in vitro Utilising 32P-γATP
Bio-Protocol, 2016 VPS34 is the only class III phosphatidylinositol-3-kinase (PI3K) in mammalian cells and produces the vast majority of cellular phosphatidylinositol-3-phosphate [PI(3)P].
Michael Munson, Ian Ganley
doaj +1 more source
Tissue environment, not ontogeny, defines murine intestinal intraepithelial T lymphocytes
eLife, 2021 Tissue-resident intestinal intraepithelial T lymphocytes (T-IEL) patrol the gut and have important roles in regulating intestinal homeostasis. T-IEL include both induced T-IEL, derived from systemic antigen-experienced lymphocytes, and natural T-IEL ...
Alejandro J Brenes +8 more
doaj +1 more source
Syk-dependent Phosphorylation of CLEC-2: A Novel Mechanism of Hem-Immunoreceptor Tyrosine-Based Activation Motif Signaling [PDF]
, 2010 The C-type lectin-like receptor CLEC-2 signals via phosphorylation of a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM).
Eble, J. A. +7 more
core +2 more sources
On the existence of Hopf bifurcations in the sequential and distributive double phosphorylation cycle [PDF]
, 2019 Protein phosphorylation cycles are important mechanisms of the post translational modification of a protein and as such an integral part of intracellular signaling and control. We consider the sequential phosphorylation and dephosphorylation of a protein
Conradi, Carsten +2 more
core +2 more sources
PKCɛ switches Aurora B specificity to exit the abscission checkpoint
Nature Communications, 2016 The Aurora B abscission checkpoint is activated when DNA is retained in the cleavage furrow on completion of anaphase. Here the authors show that PKCɛ directly phosphorylates Aurora B triggering a switch in Aurora B substrate specificity to elicit ...
Tanya Pike +4 more
doaj +1 more source
Cascades of multisite phosphorylation control Sic1 destruction at the onset of S phase. [PDF]
, 2011 Multisite phosphorylation of proteins has been proposed to transform a graded protein kinase signal into an ultrasensitive switch-like response. Although many multiphosphorylated targets have been identified, the dynamics and sequence of individual ...
Balog, Eva Rose M +8 more
core +2 more sources