Results 161 to 170 of about 40,216 (223)

Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites

open access: yesCell, 2019
Summary Tyrosine phosphorylation regulates multi-layered signaling networks with broad implications in (patho)physiology, but high-throughput methods for functional annotation of phosphotyrosine sites are lacking.
Giulia Franciosa   +2 more
exaly   +2 more sources

Expanding the genetic code of Escherichia coli with phosphotyrosine

open access: yesFEBS Letters, 2016
Protein phosphorylation is one of the most important post‐translational modifications in nature. However, the site‐specific incorporation of O‐phosphotyrosine into proteins in vivo has not yet been reported.
Dieter Soll, Chenguang Fan, Kevan Ip
exaly   +2 more sources

Monoclonal antibodies to phosphotyrosine

Journal of Immunological Methods, 1988
Phosphotyrosine coupled to KLH, BSA, and OVA was used for the production and screening of antibodies to phosphotyrosine. 800 hybridomas secreting antibodies that bound to phosphotyrosine were detected by ELISA. The most reactive 100 of these 800 were tested subsequently for their ability to bind phosphotyrosine-containing proteins on Western blots ...
J R, Glenney, L, Zokas, M P, Kamps
openaire   +2 more sources

Phosphotyrosine Profiling Using SILAC

2022
Tyrosine phosphorylation on proteins is an important posttranslational modification that regulates various processes in cells. Mass spectrometry-based phosphotyrosine profiling can reveal tyrosine kinase signaling activity in cells. Using quantitative proteomics strategies such as stable isotope labeling with amino acids in cell culture (SILAC) allows ...
Datta, Keshava K.   +2 more
openaire   +4 more sources

Design and synthesis of phosphotyrosine mimetics

Bioorganic & Medicinal Chemistry Letters, 2003
AbstractFor Abstract see ChemInform Abstract in Full Text.
Yan, Zheng   +5 more
openaire   +5 more sources

Scanning for Phosphotyrosine

Science's STKE, 2002
Steen et al. report identification of tyrosine phosphorylated peptides and the specific phosphorylated residues using a new method called phosphotyrosine-specific immonium ion (PSI) scanning. Cells treated or not treated with epidermal growth factor (EGF) provided models in which to test the technique. Steen
openaire   +2 more sources

A chromia-based sorbent for the enrichment of phosphotyrosine

Journal of Chromatography A, 2022
Developing of new core@shell particles (CSPs) bearing metal oxides on their outer surfaces is of a great interest. Such hybrid systems have many benefits, i.e., low cost, operation simplicity, chemical stability and tunability along with simple recoverability and reusability that make them suitable as dispersive solid phase extraction (DSPE) sorbents ...
Abdullah Alhendal   +4 more
openaire   +2 more sources

Preparation and characterization of antibodies to o‐phosphotyrosine and their use for identification of phosphotyrosine‐containing proteins

International Journal of Cancer, 1984
AbstractTo facilitate the identification of phosphotyrosine (Ptyr)‐containing proteins, rabbit polyclonal antibodies and mouse monoclonal antibody specifically reactive to P‐tyr were prepared by hyperimmunizing the animals with P‐tyr‐conjugated bovine serum albumin or poly‐L‐lysine.
M, Ohtsuka   +4 more
openaire   +2 more sources

Activation of a Phosphotyrosine Phosphatase by Tyrosine Phosphorylation

Science, 1993
Regulation of cell proliferation, differentiation, and metabolic homeostasis is associated with the phosphorylation and dephosphorylation of specific tyrosine residues of key regulatory proteins. The phosphotyrosine phosphatase 1D (PTP 1D) contains two amino terminally located Src homology 2 (SH2) domains and is similar to the ...
W, Vogel   +3 more
openaire   +2 more sources

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