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Nonexponential statistics of fluorescence photobleaching [PDF]

open access: yesThe Journal of Chemical Physics, 2004
In this paper, I consider theoretical models of the decay via photobleaching of a sample of surface-immobilized fluorescent molecules excited by a spatially varying laser intensity profile.
Berglund, Andrew J.
core   +5 more sources

The Impact of Photobleaching on Microarray Analysis [PDF]

open access: yesBiology, 2015
DNA-Microarrays have become a potent technology for high-throughput analysis of genetic regulation. However, the wide dynamic range of signal intensities of fluorophore-based microarrays exceeds the dynamic range of a single array scan by far, thus ...
Marcel von der Haar   +5 more
doaj   +3 more sources

Monomer and Oligomer Transition of Zinc Phthalocyanine Is Key for Photobleaching in Photodynamic Therapy [PDF]

open access: goldMolecules, 2023
Photodynamic therapy (PDT) is recognized as a powerful method to inactivate cells. However, the photosensitizer (PS), a key component of PDT, has suffered from undesired photobleaching. Photobleaching reduces reactive oxygen species (ROS) yields, leading
Dafeng Liu   +6 more
doaj   +2 more sources

Numerical Simulation of the Photobleaching Process in Laser-Induced Fluorescence Photobleaching Anemometer [PDF]

open access: yesMicromachines, 2021
At present, a novel flow diagnostic technique for micro/nanofluidics velocity measurement—laser-induced fluorescence photobleaching anemometer (LIFPA)—has been developed and successfully applied in broad areas, e.g., electrokinetic turbulence in ...
Yu Chen   +4 more
doaj   +3 more sources

Photobleaching alters the morphometric analysis of fluorescently labeled neurons and microglial cells [PDF]

open access: yesPathology and Oncology Research
Photobleaching of immunofluorescence signal is a well-known phenomenon, however, its impact on derived parameters characterizing number and shape of different cell types in tissue sections is less understood. Our aim was to determine whether the duration
Tamás F. Polgár   +11 more
doaj   +2 more sources

Photobleaching and Recovery of Symbiodiniaceae Effrenium voratum SCS01 Reveals Life Form Transformation Under Thermal Stress

open access: goldFrontiers in Marine Science, 2021
Dinoflagellates in the family Symbiodiniaceae contain a number of species and play an important role in the establishment of coral reef ecosystems in oligotrophic marine waters.
Sanqiang Gong   +14 more
doaj   +2 more sources

Safety and Effectiveness of Conventional Commercial Products for Professional Tooth Bleaching: Comparative Ex Vivo Study Using AFM Microscopy and Nanoindentation

open access: yesApplied Sciences, 2023
In modern times, patients are not only seeking oral health but also aiming for a flawless smile. Despite the progress made in aesthetic dentistry, there are instances where insufficient teeth whitening results contrast with the considerable cost of ...
Claudio Pasquale   +10 more
doaj   +1 more source

Dynamic Mode Decomposition of Fluorescence Loss in Photobleaching Microscopy Data for Model-Free Analysis of Protein Transport and Aggregation in Living Cells

open access: yesSensors, 2022
The phase separation and aggregation of proteins are hallmarks of many neurodegenerative diseases. These processes can be studied in living cells using fluorescent protein constructs and quantitative live-cell imaging techniques, such as fluorescence ...
Daniel Wüstner
doaj   +1 more source

Spectroscopic study of methylene blue photophysical properties in biological media

open access: yesBiomedical Photonics, 2023
A spectroscopic study of the photophysical properties of methylene blue (MB) in aqueous solutions was carried out. Absorption and fluorescence spectra as well as fluorescence lifetime were recorded. The concentration dependence of the intensity and shape
D. V. Pominova   +5 more
doaj   +1 more source

Photo-Switching of Protein Dynamical Collectivity

open access: yesPhotonics, 2021
We examine changes in the picosecond structural dynamics with irreversible photobleaching of red fluorescent proteins (RFP) mCherry, mOrange2 and TagRFP-T.
Mengyang Xu   +3 more
doaj   +1 more source

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