Results 1 to 10 of about 67,193 (339)

Measurement of Cyanine Dye Photobleaching in Photosensitizer Cyanine Dye Conjugates Could Help in Optimizing Light Dosimetry for Improved Photodynamic Therapy of Cancer

open access: yesMolecules, 2018
Photodynamic therapy (PDT) of cancer is dependent on three primary components: photosensitizer (PS), light and oxygen. Because these components are interdependent and vary during the dynamic process of PDT, assessing PDT efficacy may not be trivial ...
Nadine S. James   +4 more
doaj   +2 more sources

Depth-dependent autofluorescence photobleaching using 325, 473, 633, and 785 nm of porcine ear skin ex vivo [PDF]

open access: goldJournal of Biomedical Optics, 2017
. Autofluorescence photobleaching describes the decrease of fluorescence intensity of endogenous fluorophores in biological tissue upon light irradiation. The origin of autofluorescence photobleaching is not fully understood.
Johannes Schleusener   +2 more
openalex   +2 more sources

Bleach correction ImageJ plugin for compensating the photobleaching of time-lapse sequences [version 1; peer review: 3 approved]

open access: yesF1000Research, 2020
During the capturing of the time-lapse sequence of fluorescently labeled samples, fluorescence intensity exhibits decays. This phenomenon is known as 'photobleaching' and is a widely known problem in imaging in life sciences.
Kota Miura
doaj   +2 more sources

Numerical Simulation of the Photobleaching Process in Laser-Induced Fluorescence Photobleaching Anemometer [PDF]

open access: yesMicromachines, 2021
At present, a novel flow diagnostic technique for micro/nanofluidics velocity measurement—laser-induced fluorescence photobleaching anemometer (LIFPA)—has been developed and successfully applied in broad areas, e.g., electrokinetic turbulence in ...
Yu Chen   +4 more
doaj   +3 more sources

Photobleaching effect in bismuth-doped germanosilicate fibers

open access: goldOptics Express, 2015
Photoinduced reduction of absorption (photobleaching) in bismuth-doped germanosilicate fibers irradiated with 532-nm laser has been observed for the first time.
Sergei Firstov   +5 more
openalex   +2 more sources

Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity

open access: yesNature Biotechnology, 2023
Here we present a method to reduce the photobleaching of fluorescent proteins and the associated phototoxicity. It exploits a photophysical process known as reverse intersystem crossing, which we induce by near-infrared co-illumination during fluorophore
Lucie Ludvíková   +11 more
semanticscholar   +1 more source

Dynamic Mode Decomposition of Fluorescence Loss in Photobleaching Microscopy Data for Model-Free Analysis of Protein Transport and Aggregation in Living Cells

open access: yesSensors, 2022
The phase separation and aggregation of proteins are hallmarks of many neurodegenerative diseases. These processes can be studied in living cells using fluorescent protein constructs and quantitative live-cell imaging techniques, such as fluorescence ...
Daniel Wüstner
doaj   +1 more source

Spectroscopic study of methylene blue photophysical properties in biological media

open access: yesBiomedical Photonics, 2023
A spectroscopic study of the photophysical properties of methylene blue (MB) in aqueous solutions was carried out. Absorption and fluorescence spectra as well as fluorescence lifetime were recorded. The concentration dependence of the intensity and shape
D. V. Pominova   +5 more
doaj   +1 more source

Photo-Switching of Protein Dynamical Collectivity

open access: yesPhotonics, 2021
We examine changes in the picosecond structural dynamics with irreversible photobleaching of red fluorescent proteins (RFP) mCherry, mOrange2 and TagRFP-T.
Mengyang Xu   +3 more
doaj   +1 more source

Effects of the sample matrix on the photobleaching and photodegradation of toluene-derived secondary organic aerosol compounds

open access: yesAtmospheric Chemistry and Physics, 2022
. Secondary organic aerosol (SOA) generated from the photooxidation of aromatic compounds in the presence of oxides of nitrogen (NOx) is known to efficiently absorb ultraviolet and visible radiation.
Alexandra L. Klodt   +4 more
semanticscholar   +1 more source

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