Photobleaching of Chlorophyll in Light-Harvesting Complex II Increases in Lipid Environment
Frontiers in Plant Science, 2020 Excess light causes damage to the photosynthetic apparatus of plants and algae primarily via reactive oxygen species. Singlet oxygen can be formed by interaction of chlorophyll (Chl) triplet states, especially in the Photosystem II reaction center, with ...
Mónika Lingvay +4 more
semanticscholar +1 more source
Combined electromagnetic and photoreaction modeling of CLD-1 photobleaching in polymer microring resonators [PDF]
, 2005 By combining a solid-state photoreaction model with the modal solutions of an optical waveguide, we simulate the refractive index change due to the photobleaching of CLD-1 chromophores in an amorphous polycarbonate microring resonator.
Dalton, Larry R. +5 more
core +1 more source
Fluorescence recovery after photobleaching on the confocal laser-scanning microscope: generalized model without restriction on the size of the photobleached disk [PDF]
, 2011 Fluorescence recovery after photobleaching (FRAP) carried out on a confocal laser-scanning microscope (CLSM) performs well for photobleached disks that are large compared to the resolution of the bleaching beam.
Ameloot, Marcel +6 more
core +2 more sources
Photobleaching step analysis for robust determination of protein complex stoichiometries
bioRxiv, 2020 The composition of cellular structures on the nanoscale is a key determinant of macroscopic functions in cell biology and beyond. Different fluorescence single-molecule techniques have proven ideally suited for measuring protein copy numbers of cellular ...
J. Hummert +4 more
semanticscholar +1 more source
37 Years of Forest Monitoring in Switzerland: Drought Effects on Fagus sylvatica
Frontiers in Forests and Global Change, 2021 European beech is one of the most important deciduous tree species in natural forest ecosystems in Central Europe. Its dominance is now being questioned by the emerging drought damages due to the increased incidence of severe summer droughts.
Sabine Braun +4 more
doaj +1 more source
Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]
, 2012 Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe +295 more
core +2 more sources
Photobleaching with a subnanosecond laser flash [PDF]
Journal of Fluorescence, 1994 In standard fluorescence recovery after photobleaching (FRAP) applications for measuring lateral diffusion rates and adsorption/desorption kinetics of fluorescent molecules at biological or model membranes, irreversible bleaching is induced by a bright excitation flash of at least millisecond time scale. It has been presumed that the bleaching event is
Axelrod, Daniel, Yuan, Yifeng
openaire +3 more sources
Practical Implementation of Log-Scale Active Illumination Microscopy [PDF]
, 2010 Active illumination microscopy (AIM) is a method of redistributing dynamic range in a scanning microscope using real-time feedback to control illumination power on a sub-pixel time scale.
K. Chu, Kengyeh +2 more
core +3 more sources
Frontiers in Marine Science, 2020 Humic-like fluorescent dissolved organic matter (FDOM) has been widely used as tracers for bio-refractory dissolved organic matter (RDOM) to understand its dynamics in the oceans.
Yuko Omori +5 more
doaj +1 more source
EasyFRAP-web: a web-based tool for the analysis of fluorescence recovery after photobleaching data
Nucleic Acids Res., 2018 Understanding protein dynamics is crucial in order to elucidate protein function and interactions. Advances in modern microscopy facilitate the exploration of the mobility of fluorescently tagged proteins within living cells.
Grigorios Koulouras +5 more
semanticscholar +1 more source