Results 31 to 40 of about 67,193 (339)

An Approach for Characterizing and Comparing Hyperspectral Microscopy Systems

Sensors, 2013
Hyperspectral imaging and analysis approaches offer accurate detection and quantification of fluorescently-labeled proteins and cells in highly autofluorescent tissues.
Silas J. Leavesley, Thomas C. Rich, Diego F. Alvarez, Peter Favreau, Ashley S. Lindsey, Naga S. Annamdevula, Brenner Sweat   +6 more
doaj   +1 more source

Low Power Consumption Mode Switch Based on Three-Dimensional Polymer Mach-Zehnder Interferometer

IEEE Photonics Journal, 2020
We present a polymer thermo-optic mode switch based on the structure of two vertical Mach-Zehnder interferometers (MZIs). It can achieve a rapid switch between ${E_{00}}$, ${E_{01}}$, ${E_{10}}$ and ${E_{11}}$ with the different heaters turning on.
Jiawen Lv, Tianhang Lian, Baizhu Lin, Yue Yang, Yue Cao, Xianwang Yang, Yunji Yi, Fei Wang, Daming Zhang   +8 more
doaj   +1 more source

Photobleaching Photoacid Generator.

Journal of Photopolymer Science and Technology, 2001
A cyclopropyl group was introduced into the photoacid generator in order to give the photobleaching property at 193nm. Cyclopropyldiphenylsulfonium trifluoromethanesulfonate (CpDP-Tf) was synthesized for this photobleaching effect. This compound is stable up to 165°C. The absorbance of CpDP-Tf at 193nm decreases upon exposure.
Kim, J.-B., Jang, Ji-Hyun, Kim, H.-W., Woo, S.-G.   +3 more
openaire   +2 more sources

Photobleaching in STED nanoscopy and its dependence on the photon flux applied for reversible silencing of the fluorophore

Scientific Reports, 2017
In STED (stimulated emission depletion) nanoscopy, the resolution and signal are limited by the fluorophore de-excitation efficiency and photobleaching. Here, we investigated their dependence on the pulse duration and power of the applied STED light for ...
Joanna Oracz, V. Westphal, C. Radzewicz, S. Sahl, S. Hell   +4 more
semanticscholar   +1 more source

Photobleaching Effect on the Sensitivity Calibration at 638 nm of a Phosphorus-Doped Single-Mode Optical Fiber Dosimeter

Sensors
We investigated the influence of the photobleaching (PB) effect on the dosimetry performances of a phosphosilicate single-mode optical fiber (core diameter of 6.6 µm) operated at 638 nm, within the framework of the LUMINA project.
Fiammetta Fricano, Adriana Morana, Martin Roche, Alberto Facchini, Gilles Mélin, Florence Clément, Nicolas Balcon, Julien Mekki, Emmanuel Marin, Youcef Ouerdane, Aziz Boukenter, Thierry Robin, Sylvain Girard   +12 more
doaj   +1 more source

An improved method for whole-mount in situ hybridization in regenerating tails of Xenopus laevis tadpoles

Frontiers in Cell and Developmental Biology
Whole-mount in situ hybridization (WISH) is a widely used method that supports the concept of “seeing is believing” by enabling the visualization of gene expression patterns in whole-mount multicellular samples or sections. This technique is essential in
A. D. Shitikov, E. A. Parshina, A. G. Zaraisky, A. G. Zaraisky, M. B. Tereshina, M. B. Tereshina   +5 more
doaj   +1 more source

Influence of cell growth conditions and medium composition on EGFP photostability in live cells

BioTechniques, 2015
Photostability is a key characteristic of fluorescent proteins. It was recently demonstrated that green fluorescent protein (GFP) photobleaching in live cells can be suppressed by changes in medium composition. Here we show that Ham's F12 medium provides
Anastasia V. Mamontova, Alexey M. Bogdanov, Konstantin A. Lukyanov   +2 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Antifouling PEG Coatings by Thiol‐Acrylate Conjugate Addition Reactions for Generation of Protein Patterns via Photobleaching‐Induced Protein Binding (PiPB) BioBitmaps Using Maskless Projection Lithography

Advanced Materials Interfaces
Photobleaching‐induced protein binding (PiPB) is a light‐based molecular patterning technique that is introduced as Protein Bitmaps. This technique has significant applications in immunoassays and cell‐substrate interactions.
Ali Usama, Evelin Schäfer, Pang Zhu, Qingchuan Song, Alexander Weißbach, Dorothea Helmer, Joachim Wollschläger, Changjiang You, Martin Steinhart, Pegah Pezeshkpour, Peilong Hou, Bastian E. Rapp   +11 more
doaj   +1 more source

Bleaching‐Resistant Super‐Resolution Fluorescence Microscopy

Advanced Science, 2022
Photobleaching is the permanent loss of fluorescence after extended exposure to light and is a major limiting factor in super‐resolution microscopy (SRM) that restricts spatiotemporal resolution and observation time.
Jiwoong Kwon, Mohamed Saleh Elgawish, Sang‐Hee Shim   +2 more
doaj   +1 more source