Results 51 to 60 of about 110,959 (369)

Primary cultures of chick osteocytes retain functional gap junctions between osteocytes and between osteocytes and osteoblasts [PDF]

, 2008
The inaccessibility of osteocytes due to their embedment in the calcified bone matrix in vivo has precluded direct demonstration that osteocytes use gap junctions as a means of intercellular communication.
Ishihara, Yoshihito, Kamioka, Hiroshi, Lim, Soo-Siang, Murshid, Sakhr A., Ris, Hans, Sugawara, Yasuyo, Yamamoto, Teruko Takano   +6 more
core   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Light-Induced Effects in Amorphous Chalcogenide Glasses: Femtoseconds to Seconds

Physics, 2021
Amorphous chalcogenide glasses are intrinsically metastable, highly photosensitive, and therefore exhibit numerous light-induced effects upon bandgap and sub-bandgap illumination.
Pritam Khan, K. V. Adarsh
doaj   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Antifouling PEG Coatings by Thiol‐Acrylate Conjugate Addition Reactions for Generation of Protein Patterns via Photobleaching‐Induced Protein Binding (PiPB) BioBitmaps Using Maskless Projection Lithography

Advanced Materials Interfaces
Photobleaching‐induced protein binding (PiPB) is a light‐based molecular patterning technique that is introduced as Protein Bitmaps. This technique has significant applications in immunoassays and cell‐substrate interactions.
Ali Usama, Evelin Schäfer, Pang Zhu, Qingchuan Song, Alexander Weißbach, Dorothea Helmer, Joachim Wollschläger, Changjiang You, Martin Steinhart, Pegah Pezeshkpour, Peilong Hou, Bastian E. Rapp   +11 more
doaj   +1 more source

An improved method for whole-mount in situ hybridization in regenerating tails of Xenopus laevis tadpoles

Frontiers in Cell and Developmental Biology
Whole-mount in situ hybridization (WISH) is a widely used method that supports the concept of “seeing is believing” by enabling the visualization of gene expression patterns in whole-mount multicellular samples or sections. This technique is essential in
A. D. Shitikov, E. A. Parshina, A. G. Zaraisky, A. G. Zaraisky, M. B. Tereshina, M. B. Tereshina   +5 more
doaj   +1 more source

Bleaching‐Resistant Super‐Resolution Fluorescence Microscopy

Advanced Science, 2022
Photobleaching is the permanent loss of fluorescence after extended exposure to light and is a major limiting factor in super‐resolution microscopy (SRM) that restricts spatiotemporal resolution and observation time.
Jiwoong Kwon, Mohamed Saleh Elgawish, Sang‐Hee Shim   +2 more
doaj   +1 more source

Individual IKs channels at the surface of mammalian cells contain two KCNE1 accessory subunits. [PDF]

, 2014
KCNE1 (E1) β-subunits assemble with KCNQ1 (Q1) voltage-gated K(+) channel α-subunits to form IKslow (IKs) channels in the heart and ear. The number of E1 subunits in IKs channels has been an issue of ongoing debate.
Dai, Hui, Goldstein, Steve AN, Plant, Leigh D, Xiong, Dazhi   +3 more
core  

A FRAP model to investigate reaction-diffusion of proteins within a bounded domain: a theoretical approach

, 2008
Temporally and spatially resolved measurements of protein transport inside cells provide important clues to the functional architecture and dynamics of biological systems. Fluorescence Recovery After Photobleaching (FRAP) technique has been used over the
Axelrod, Beaudouin, Blonk, Braeckmans, Braga, Braga, Carmo-Fonseca, Carrero, Carrero, Carrero, Carslaw, Crank, Dundr, Edidin, George D. Tsibidis, Hinow, Houtsmuller, Jacobson, Jacques, Jacques, Jorge Ripoll, Koppel, Lele, Lippincott-Schwartz, Meyvis, Peters, Peters, Phair, Phair, Reits, Soumpasis, Sprague, Sprague, Sprague, Tang, Tardy, Th’ng, Wedekind, Wedekind, Weiss, White   +40 more
core   +1 more source

A novel method to accurately locate and count large numbers of steps by photobleaching

Molecular Biology of the Cell, 2016
A new Bayesian photobleaching trace analysis method that is computationally inexpensive can be used to treat blinking, reactivation, and overlapping events and reliably detect up to 50 fluorophores even for low signal-to-noise ratios.
K. Tsekouras, Thomas C. Custer, Hossein Jashnsaz, N. Walter, S. Pressé   +4 more
semanticscholar   +1 more source