Results 121 to 130 of about 14,720,819 (304)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Wetland Dwellers: Sound Performance Walk

, 2022
A Performance Sound walk Shire Brook Valley and Woodhouse Washlands in South Yorkshire with Gary Stewart (Goldsmiths), Sam Vardy (SHU), Phil Warren (SU), Sheffield wildlife trust.
McCloskey, P.
core  

Morphological aspects of the quadriceps and their relationship with torque and power parameters in male soccer players at the beginning of the season

Apunts Sports Medicine
Background/Objective: The aim of this study was to evaluate the relationship between the morphological characteristics of the quadriceps and torque parameters and anaerobic fitness in soccer players at the beginning of the season.
Thaissa Gonzaga Blanco Pardo, João Cláudio Braga Pereira Machado, Stephany Fernandes Rodrigues da Rocha, Matheus Henrique Leão, Rafael Martins da Costa, Rodrigo Ghedini Gheller, Mateus Rossato   +6 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Relationship between tests of physical qualities and physical match performance in elite Rugby League players

, 2013
Previous investigators have reported significant relationships between tests of physical qualities and physical match performance in high-intensity intermittent team sport (e.g. soccer) players.
Gabbett, Tim J., Kemp, Justin G., Lorenzen, Christian, Stein, Josh G.   +3 more
core   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Physical and Physiological Characteristics of Elite CrossFit Athletes

Sports
Due to little available research on elite CrossFit athletes, the present investigation was undertaken to provide knowledge about their physical and physiological characteristics.
Bram Sauvé, Magnus Haugan, Gøran Paulsen   +2 more
doaj   +1 more source

Physically interpretable performance metrics for clustering

The Journal of Chemical Physics
Clustering is a type of machine learning technique, which is used to group huge amounts of data based on their similarity into separate groups or clusters. Clustering is a very important task that is nowadays used to analyze the huge and diverse amount of data coming out of molecular dynamics (MD) simulations.
Kinjal Mondal, Jeffery B. Klauda
openaire   +2 more sources

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source