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Isolation and properties of phytoene
Archives of Biochemistry and Biophysics, 1954Abstract Phytoene, a new noncrystalline, colorless polyene, has been isolated from tomatoes and carrot oil by an improved adsorptive procedure. It was adsorbed less strongly than the known carotenes and polyenes on magnesia and alumina. It produced a very weak Carr-Price reaction, but it did give a characteristic color reaction with the Liebermann ...
W J, RABOURN +2 more
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Phytoene Accumulation in Sunflower Decreases the Transcript Levels of the Phytoene Synthase Gene
Plant Growth Regulation, 2006The non dormant-1 (nd-1) mutant of sunflower (Helianthus annuus L.) and fluridone-treated plants were used to investigate the effect of phytoene accumulation on the transcript levels of the phytoene synthase (Ha-PSY) gene. A deficiency at the gene coding for ζ-carotene desaturase (ZDS) characterises the nd-1 mutant, which displays an accumulation of ζ ...
L. Campisi +5 more
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The stereochemistry of phytoene biosynthesis
Proceedings of the Royal Society of London. Series B. Biological Sciences, 1966Abstract Experiments with carrot root preparations have demonstrated that all the eliminations of hydrogen at C-4 of mevalonic acid during its conversion into phytoene have the same stereospecificity; the 4-R hydrogen is retained and the 4Shydrogen is eliminated.
Trevor Walworth Goodwin +1 more
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The occurrence of phytoene in various plant materials
Archives of Biochemistry and Biophysics, 1953Abstract A method of assay for phytoene in plant materials is presented which involves isooctane-ethanol extraction, adsorption of the interfering substances on a magnesia-Super Cel column, and absorptimetric determination of phytoene at 286 mμ. Twenty plant materials were analyzed for phytoene by this method.
W J, RABOURN, F W, QUACKENBUSH
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Biochemistry, 2003
Phytoene synthase (PSase) catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to phytoene. These reactions constitute the first pathway specific step in carotenoid biosynthesis.
Dirk, Iwata-Reuyl +3 more
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Phytoene synthase (PSase) catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to phytoene. These reactions constitute the first pathway specific step in carotenoid biosynthesis.
Dirk, Iwata-Reuyl +3 more
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Evidence for phytoene in euglena mutants
Plant Science Letters, 1974Abstract The hydrocarbon carotene fractions of several non-photosynthetic mutants of Euglena gracilis var. bacillaris and of Astasia longa were examined. Evidence for the presence of phytoene, the presumed precursor of all C-40 carotenoids, is reported from 3 mutants, SM-L1, PBZ-G3 and HB-G, which synthesize unsaturated C-40 polyenes; they also
J.A. Gross, Richard J. Stroz
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Journal of Agricultural and Food Chemistry, 2011
Phytoene synthase (PSY) and phytoene desaturase (PDS), which catalyze the first and second steps of the carotenoid biosynthetic pathway, respectively, are key enzymes for the accumulation of carotenoids in many plants. We isolated 2 partial cDNAs encoding PSY (AsPSY-1 and AsPSY-2) and a partial cDNA encoding PDS (AsPDS) from Allium sativum. They shared
Pham Anh, Tuan +5 more
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Phytoene synthase (PSY) and phytoene desaturase (PDS), which catalyze the first and second steps of the carotenoid biosynthetic pathway, respectively, are key enzymes for the accumulation of carotenoids in many plants. We isolated 2 partial cDNAs encoding PSY (AsPSY-1 and AsPSY-2) and a partial cDNA encoding PDS (AsPDS) from Allium sativum. They shared
Pham Anh, Tuan +5 more
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The purification of phytoene dehydrogenase from Phycomyces blakesleeanus
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1994The carotenogenic enzyme phytoene dehydrogenase has been purified from the C9carR21(-) (lycopene-accumulating) mutant of the filamentous fungus Phycomyces blakesleeanus. Solubilization of the membrane-bound enzyme with 1% Tween-60 was followed by a 250-fold purification to homogeneity using polyethylene glycol precipitation, CM-Sepharose, gel ...
P D, Fraser, P M, Bramley
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Occurrence of dehydrosqualene (C30 phytoene) in Staphylococcus aureus
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1968Abstract 1. 1. A mutant of Staphylococcus aureus 209 P, which had lost the ability to synthesize colored carotenoids, accumulated a phytoene-like compound. By ultraviolet, infrared, nuclear magnetic resonance and mass spectra, together with chemical analyses, the structure of this compound was established to be 2,6,10,15,19,23-hexamethyl-2, 6 ...
G, Suzue, K, Tsukada, S, Tanaka
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β-Carotene, Canthaxanthin, and Phytoene
2021Carotenoid pigments have a recognized role in the prevention of photosensitization in photosynthetic organisms, both unicellular and multicellular. The ability of carotenoid pigments to prevent the development of tumors other than skin tumors has also been studied.
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