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Carotenoid Accumulation and Characterization of cDNAs Encoding Phytoene Synthase and Phytoene Desaturase in Garlic (Allium sativum)

Journal of Agricultural and Food Chemistry, 2011
Phytoene synthase (PSY) and phytoene desaturase (PDS), which catalyze the first and second steps of the carotenoid biosynthetic pathway, respectively, are key enzymes for the accumulation of carotenoids in many plants. We isolated 2 partial cDNAs encoding PSY (AsPSY-1 and AsPSY-2) and a partial cDNA encoding PDS (AsPDS) from Allium sativum. They shared
Pham Anh, Tuan   +5 more
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The purification of phytoene dehydrogenase from Phycomyces blakesleeanus

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1994
The carotenogenic enzyme phytoene dehydrogenase has been purified from the C9carR21(-) (lycopene-accumulating) mutant of the filamentous fungus Phycomyces blakesleeanus. Solubilization of the membrane-bound enzyme with 1% Tween-60 was followed by a 250-fold purification to homogeneity using polyethylene glycol precipitation, CM-Sepharose, gel ...
P D, Fraser, P M, Bramley
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Occurrence of dehydrosqualene (C30 phytoene) in Staphylococcus aureus

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1968
Abstract 1. 1. A mutant of Staphylococcus aureus 209 P, which had lost the ability to synthesize colored carotenoids, accumulated a phytoene-like compound. By ultraviolet, infrared, nuclear magnetic resonance and mass spectra, together with chemical analyses, the structure of this compound was established to be 2,6,10,15,19,23-hexamethyl-2, 6 ...
G, Suzue, K, Tsukada, S, Tanaka
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An Enzyme Complex for the Dehydrogenation of Phytoene in Phycomyces

European Journal of Biochemistry, 1976
Phycomyces strain C5, carrying mutation carB10, accumulates phytoene instead of β‐carotene. Heterokaryons containing C5 nuclei and different other nuclei carrying the wild type carB allele accumulate significant amounts of phytofluene, ζ‐carotene and neurosporene.
C M, Aragon   +3 more
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β-Carotene, Canthaxanthin, and Phytoene

2021
Carotenoid pigments have a recognized role in the prevention of photosensitization in photosynthetic organisms, both unicellular and multicellular. The ability of carotenoid pigments to prevent the development of tumors other than skin tumors has also been studied.
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cis- and trans-phytoene in Verticillium agaricinum

Phytochemistry, 1973
Abstract cis -Phytoene and trans -phytofluene were identified in illuminated cultures of Verticillium agaricinum in addition to the other ...
L.R.G. Valadon   +3 more
openaire   +1 more source

[26] Enzymatic synthesis of phytoene

1985
Publisher Summary This chapter focuses on enzymatic synthesis of phytoene. Enzyme systems in the chromoplasts of tomato fruits and in extracts of spinach leaves convert isopentenyl pyrophosphate to phytoene by the reactions are discussed in the chapter.
Benjamin L. Jones, John W. Porter
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Genetic control of phytoene dehydrogenation in phycomyces

Plant Science Letters, 1974
Abstract Strains of Phycomyces blakesleeanus carrying mutations in gene carB accumulate large amounts of phytoene. A leaky carB mutant, isolated from a red, lycopene-producing strain, accumulates decreasing amounts of phytoene, phytofluene, ζ-carotene, neurosporene, and lycopene.
A.P. Eslava, E. Cerdá-Olmedo
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cDNA cloning and expression of the phytoene synthase gene in sunflower

Journal of Plant Physiology, 2005
A PCR approach was used to isolate a phytoene synthase (Psy) cDNA from sunflower (Helianthus annuus L.). A reconstructed full-length sequence (1598 bp) of the Psy cDNA was obtained; it contained a 1242 bp CDS, 172-nucleotides of 5'-untranslated region (UTR), and 170-nucleotides of 3'-UTR.
SALVINI, Mariangela   +3 more
openaire   +4 more sources

[Advances in phytoene dehydrogenase - A review].

Wei sheng wu xue bao = Acta microbiologica Sinica, 2018
Carotenoids, as a group of over 700 valuable unsaturated terpene compounds classified as carotene and xanthophyll family, are endowed with powerful nutritional value. Phytoene dehydrogenase is the key rate-limiting enzyme in carotenoids biosynthesis pathway, involved in catalyzing the conversion from colorless hydrocarbon phytoene to other pigmented ...
Chunji, Li, Bingxue, Li, Xiaori, Han
openaire   +1 more source

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