The Bacterial Phytoene Desaturase-Encoding Gene (CRTI) is an Efficient Selectable Marker for the Genetic Transformation of Eukaryotic Microalgae [PDF]
Metabolites, 2019 Genetic manipulation shows great promise to further boost the productivity of microalgae-based compounds. However, selection of microalgal transformants depends mainly on the use of antibiotics, which have raised concerns about their potential impacts on
Marta Vila, Javier Vigara, Rosa Leon
exaly +4 more sources
Knockout of phytoene desaturase gene using CRISPR/Cas9 in highbush blueberry [PDF]
Frontiers in Plant Science, 2022 Among the New Plant Breeding Techniques (NPBTs), the CRISPR/Cas9 system represents a useful tool for target gene editing, improving the traits of the plants rapidly.
Giuseppe Vaia +4 more
doaj +2 more sources
CRISPR/Cas9-mediated editing of PHYTOENE DESATURASE gene in onion (Allium cepa L.) [PDF]
Frontiers in Plant Science, 2023 IntroductionClustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9) is a precise genome editing tool used to introduce genetic modifications in a wide range of crop species.
Pawan Mainkar +3 more
doaj +2 more sources
In Silico Structure-Guided Optimization and Molecular Simulation Studies of 3-Phenoxy-4-(3-trifluoromethylphenyl)pyridazines as Potent Phytoene Desaturase Inhibitors [PDF]
Molecules, 2021 A series of novel 3-phenoxy-4-(3-trifluoromethylphenyl)pyridazines 2–5 were designed, based on the structure of our previous lead compound 1 through the in silico structure-guided optimization approach. The results showed that some of these new compounds
Lijun Yang +7 more
doaj +2 more sources
Plant-type phytoene desaturase: Functional evaluation of structural implications. [PDF]
PLoS ONE, 2017 Phytoene desaturase (PDS) is an essential plant carotenoid biosynthetic enzyme and a prominent target of certain inhibitors, such as norflurazon, acting as bleaching herbicides.
Julian Koschmieder +6 more
doaj +2 more sources
Multiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canephora [PDF]
Scientific Reports, 2022 Coffea canephora (2n = 2x = 22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than
Tatiane Casarin +9 more
doaj +2 more sources
Efficient CRISPR/Cas9 Genome Editing of Phytoene desaturase in Cassava [PDF]
Frontiers in Plant Science, 2017 CRISPR/Cas9 has become a powerful genome-editing tool for introducing genetic changes into crop species. In order to develop capacity for CRISPR/Cas9 technology in the tropical staple cassava (Manihot esculenta), the Phytoene desaturase (MePDS) gene was ...
John Odipio +8 more
doaj +2 more sources
Phytoene Desaturase from Oryza sativa: Oligomeric Assembly, Membrane Association and Preliminary 3D-Analysis. [PDF]
PLoS ONE, 2015 Recombinant phytoene desaturase (PDS-His6) from rice was purified to near-homogeneity and shown to be enzymatically active in a biphasic, liposome-based assay system. The protein contains FAD as the sole protein-bound redox-cofactor.
Sandra Gemmecker +9 more
doaj +2 more sources
CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry [PDF]
Plant Methods, 2019 Background Gene editing using CRISPR/Cas9 is a simple and powerful tool for elucidating genetic controls and for crop improvement and its use has been reported in a growing number of important food crops, including recently Fragaria.
Fiona M. Wilson +4 more
doaj +2 more sources
Silencing of the phytoene desaturase (PDS) gene affects the expression of fruit-ripening genes in tomatoes [PDF]
Plant Methods, 2019 Background Past research has shown that virus-induced phytoene desaturase (PDS) gene silencing via agroinjection in the attached and detached fruit of tomato plants results in a pale-yellow fruit phenotype. Although the PDS gene is often used as a marker
Aung Htay Naing +6 more
doaj +2 more sources