The carotenoids metabolism during tomato fruit ripening: insights into multi-level regulatory network. [PDF]
Wang J, Wu Y, Yu J.
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Characterization of Phytoene Desaturase Knockout Carotenoid-Deficient Microalgal Mutants Generated by Cas9-Ribonucleoprotein Complexes. [PDF]
Molina-Márquez A +5 more
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Enzyme-constrained genome-scale model of Yarrowia lipolytica predicts growth-phase specific metabolic engineering targets. [PDF]
De Biaggi JS +4 more
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Engineering artificial 5' regulatory sequences for thermostable protein expression in the extremophile <i>Thermus thermophilus</i>. [PDF]
Fai Alex Wong C +4 more
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Carotenoid biosynthesis in Prunus species: from pathway and accumulation structure to diverse pigmentation. [PDF]
Baz NM +7 more
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Metabolic engineering of endogenous MEP pathway for enhanced lycopene production in <i>Escherichia coli</i>. [PDF]
Xu X +6 more
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Momordica charantia, a tropical plant, produces a fruit that has a β-carotene concentration five times higher than that of carrot. To elucidate the molecular basis of β-carotene accumulation in M. charantia, the gene expression levels of phytoene synthase (McPSY) and phytoene desaturase (McPDS) were determined.
Pham Anh, Tuan +4 more
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cDNA cloning and expression of the phytoene synthase gene in sunflower
Journal of Plant Physiology, 2005A PCR approach was used to isolate a phytoene synthase (Psy) cDNA from sunflower (Helianthus annuus L.). A reconstructed full-length sequence (1598 bp) of the Psy cDNA was obtained; it contained a 1242 bp CDS, 172-nucleotides of 5'-untranslated region (UTR), and 170-nucleotides of 3'-UTR.
SALVINI, Mariangela +3 more
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Envelope membranes of spinach chloroplasts contain appreciable activities of the carotenogenic enzymes phytoene synthase (formation of phytoene by condensation of two molecules geranylgeranyl pyrophosphate) and phytoene dehydrogenase (formation of lycopene from phytoene), plus a phosphatase activity.
F, Lütke-Brinkhaus +3 more
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Phytoene synthase activity in tomato chloroplasts is membrane-associated, requiring treatment with high ionic strength buffer or mild non-ionic detergent for solubilisation. Using a combination of ammonium sulphate precipitation, cation and anion exchange, dye-ligand and hydrophobic interaction chromatography, phytoene synthase has been purified 600 ...
P D, Fraser, W, Schuch, P M, Bramley
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