Results 111 to 120 of about 45,758 (267)

Engineering Methylotrophic Yeasts as Cell Factories for Chemical Production Using Methanol as a Feedstock

GCB Bioenergy, Volume 17, Issue 8, August 2025.
Methanol, a sustainable and abundant one‐carbon feedstock, can be utilized by natural methylotrophic yeasts to synthesize valuable chemicals. However, methanol‐based manufacturing with these yeasts faces several challenges, including low assimilation efficiency, carbon loss, and methanol toxicity. This review summarizes and discusses recent engineering
Shuxian Wang, Jiayu Fang, Yanping Zhang, Yin Li, Taicheng Zhu   +4 more
wiley   +1 more source

Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate

Biotechnology for Biofuels, 2018
BackgroundInterests in renewable fuels have exploded in recent years as the serious effects of global climate change become apparent. Microbial production of high-energy fuels by economically efficient bioprocesses has emerged as an attractive ...
Wiparat Siripong, Philip F. Wolf, Theodora Puspowangi Kusumoputri, J. Downes, K. Kocharin, S. Tanapongpipat, Weerawat Runguphan   +6 more
semanticscholar   +1 more source

The receptor‐like protein RXEG1 confers broad‐spectrum resistance in soybean and cotton by recognizing microbial glycoside hydrolase 12 proteins

Plant Biotechnology Journal, Volume 23, Issue 8, Page 3417-3430, August 2025.
Summary Plants diseases cause substantial crop yield loss and threaten food security. Enhancing plant genetic resistance is a major strategy to mitigate the impact of plant diseases on agricultural production. The leucine‐rich repeat receptor‐like protein RXEG1 recognizes the glycoside hydrolase 12 (GH12) protein XEG1 secreted by Phytophthora sojae to ...
Mengzhu Zeng, Ping Qiu, Haibing Ouyang, Yujing Sun, Zhiyuan Chen, Yuanyuan Cheng, Mingmei Zhang, Yu Ye, Lei Wang, Zuling Zheng, Hao Wang, Tianyao Liu, Wei Zhao, Kaixuan Duan, Yan Wang, Longfu Zhu, Yuanchao Wang   +16 more
wiley   +1 more source

An improved secretion signal enhances the secretion of model proteins from Pichia pastoris

Microbial Cell Factories, 2018
BackgroundProteins can be secreted from a host organism with the aid of N-terminal secretion signals. The budding yeast Pichia pastoris (Komagataella sp.) is widely employed to secrete proteins of academic and industrial interest.
Juan J. Barrero, Jason C. Casler, F. Valero, P. Ferrer, B. Glick   +4 more
semanticscholar   +1 more source

Immunological evaluation of fusion protein of Brugia malayi abundant larval protein transcript-2 (BmALT-2) and Tuftsin in experimental mice model

Parasite Epidemiology and Control, 2019
Introduction: Filariasis, a neglected tropical helminth disease needs vaccine besides mass drug administration for its successful eradication. Methods: An attempt was made to produce a fusion protein (P-TUFT-ALT-2) of abundant larval transcript protein-2
Rajkumar Paul, Meganathan Ilamaran, Vishal Khatri, Nitin Amdare, Maryada Venkata Rami Reddy, Perumal Kaliraj   +5 more
doaj   +1 more source

CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

Microbial Cell Factories, 2019
BackgroundPichia pastoris (syn. Komagataella phaffii) is a widely used generally recognized as safe host for heterologous expression of proteins in both industry and academia.
Qi Liu, Xiaona Shi, Lili Song, Haifeng Liu, Xiangshan Zhou, Qiyao Wang, Yuanxing Zhang, Menghao Cai   +7 more
semanticscholar   +1 more source

Expression and Purification of Mammalian Calreticulin in Pichia pastoris

Protein Expression and Purification, 2000
Calreticulin is a 46-kDa Ca(2+)-binding chaperone of the endoplasmic reticulum membranes. The protein binds Ca(2+) with high capacity, affects intracellular Ca(2+) homeostasis, and functions as a lectin-like chaperone. In this study, we describe expression and purification procedures for the isolation of recombinant rabbit calreticulin.
Andrin, C, Corbett, E, Johnson, S, Dabrowska, M, Campbell, I, Eggleton, P, Opas, M, Michalak, M   +7 more
openaire   +4 more sources

Influence of Co-feeding Methanol-sorbitol Ratio on Production of Human Insulin Precursor Expressed by Mut+ Pichia pastoris

Hayati Journal of Biosciences
An increasing number of diabetic patients and the demand for insulin encourage the development of recombinant insulin production on a large scale. Human insulin precursor (HIP) expressed by Mut+ Pichia pastoris using methanol as an inducer was developed.
Dian Japany Puspitasari, Anis Herliyati Mahsunah, Dini Nurdiani, Rika Indri Astuti, Anja Meryandini   +4 more
doaj   +1 more source

Production of protein-based polymers in Pichia pastoris

Biotechnology Advances, 2019
Materials science and genetic engineering have joined forces over the last three decades in the development of so-called protein-based polymers. These are proteins, typically with repetitive amino acid sequences, that have such physical properties that they can be used as functional materials. Well-known natural examples are collagen, silk, and elastin,
Werten, Marc W.T., Eggink, Gerrit, Cohen Stuart, Martien A., de Wolf, Frits A.   +3 more
openaire   +3 more sources

Cloning of a novel constitutively expressed pectate lyase gene pelB from Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of the gene product expressed in Pichia pastoris [PDF]

, 1995
Wenjin Guo, Luı́s González-Candelas, P.E. Kolattukudy   +2 more
openalex   +1 more source