Results 1 to 10 of about 10,899,828 (149)

PieParty: visualizing cells from scRNA-seq data as pie charts [PDF]

Life Science Alliance, 2021
Single-cell RNA sequencing (scRNA-seq) has been a transformative technology in many research fields. Dimensional reduction techniques such as UMAP and tSNE are used to visualize scRNA-seq data in two or three dimensions for cells to be clustered in biologically meaningful ways.
Stefan Kurtenbach, James J Dollar, Anthony M Cruz, Michael A Durante, Christina L Decatur, J William Harbour   +5 more
semanticscholar   +4 more sources

PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells [PDF]

Biophysical Journal, 2020
ABSTRACTWe report the use of pulsed interleaved excitation-fluorescence lifetime imaging microscopy (PIE-FLIM) to measure the activities of two different biosensor probes simultaneously in single living cells. Many genetically encoded biosensors rely on the measurement of Förster resonance energy transfer (FRET) to detect changes in biosensor ...
Reissaus, Christopher A., Day, Kathleen H., Mirmira, Raghavendra G., Dunn, Kenneth W., Pavalko, Fredrick M., Day, Richard N.   +5 more
openaire   +4 more sources

PIE-seq: identifying RNA-binding protein targets by dual RNA-deaminase editing and sequencing

Nature Communications, 2023
RNA-binding proteins (RBPs) are essential for gene regulation, but it remains a challenge to identify their RNA targets across cell types. Here we present PIE-Seq to investigate Protein-RNA Interaction with dual-deaminase Editing and Sequencing by ...
Xiangbin Ruan, Kaining Hu, Xiaochang Zhang   +2 more
doaj   +2 more sources

Ligilactobacillus salivarius Strains Isolated From the Porcine Gut Modulate Innate Immune Responses in Epithelial Cells and Improve Protection Against Intestinal Viral-Bacterial Superinfection

Frontiers in Immunology, 2021
Previously, we constructed a library of Ligilactobacillus salivarius strains from the intestine of wakame-fed pigs and reported a strain-dependent capacity to modulate IFN-β expression in porcine intestinal epithelial (PIE) cells.
Yuhki Indo, Yuhki Indo, Shugo Kitahara, Shugo Kitahara, Mikado Tomokiyo, Mikado Tomokiyo, Shota Araki, Md. Aminul Islam, Md. Aminul Islam, Md. Aminul Islam, Binghui Zhou, Binghui Zhou, Leonardo Albarracin, Leonardo Albarracin, Leonardo Albarracin, Ayako Miyazaki, Wakako Ikeda-Ohtsubo, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Tomonori Nochi, Takato Takenouchi, Hirohide Uenishi, Hisashi Aso, Hisashi Aso, Hideki Takahashi, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Julio Villena, Haruki Kitazawa, Haruki Kitazawa   +30 more
doaj   +2 more sources

PIE-1 Translation in the Germline Lineage Contributes to PIE-1 Asymmetry in the Early Caenorhabditis elegans Embryo

G3: Genes, Genomes, Genetics, 2018
In the C. elegans embryo, the germline lineage is established through successive asymmetric cell divisions that each generate a somatic and a germline daughter cell.
Timothy J. Gauvin, Bingjie Han, Michael J. Sun, Erik E. Griffin   +3 more
doaj   +2 more sources

Immunoregulatory effect of bifidobacteria strains in porcine intestinal epithelial cells through modulation of ubiquitin-editing enzyme A20 expression. [PDF]

PLoS ONE, 2013
BACKGROUND: We previously showed that evaluation of anti-inflammatory activities of lactic acid bacteria in porcine intestinal epithelial (PIE) cells is useful for selecting potentially immunobiotic strains. OBJECTIVE: The aims of the present study were:
Yohsuke Tomosada, Julio Villena, Kozue Murata, Eriko Chiba, Tomoyuki Shimazu, Hisashi Aso, Noriyuki Iwabuchi, Jin-zhong Xiao, Tadao Saito, Haruki Kitazawa   +9 more
doaj   +2 more sources

Lactobacillus delbrueckii CRL 581 Differentially Modulates TLR3-Triggered Antiviral Innate Immune Response in Intestinal Epithelial Cells and Macrophages

Microorganisms, 2021
Lactobacillus delbrueckii subsp. lactis CRL 581 beneficially modulates the intestinal antiviral innate immune response triggered by the Toll-like receptor 3 (TLR3) agonist poly(I:C) in vivo.
Mariano Elean, Leonardo Albarracin, Kohtaro Fukuyama, Binghui Zhou, Mikado Tomokiyo, Shugo Kitahara, Shota Araki, Yoshihito Suda, Lucila Saavedra, Julio Villena, Elvira M. Hebert, Haruki Kitazawa   +11 more
doaj   +2 more sources

Tumor immune microenvironment score predicts efficacy of immune checkpoint inhibitors-based regimens in advanced non-small cell lung cancer [PDF]

Journal of Translational Medicine
Background Immune checkpoint inhibitor (ICI)-based regimens have become the standard first-line treatment for advanced non-small cell lung cancer (NSCLC).
Jiacheng Dai, Huan Yan, Yangqian Chen, Yuda Zhang, Zhe Huang, Zhaohui Ruan, Fang Tian, Haoyue Qin, Qinqin Xu, Jin Wang, Xing Li, Peng Cheng, Changbin Zhu, Nong Yang, Liang Zeng, Yongchang Zhang   +15 more
doaj   +2 more sources

Propofol Inhibits Proliferation and Augments the Anti-Tumor Effect of Doxorubicin and Paclitaxel Partly Through Promoting Ferroptosis in Triple-Negative Breast Cancer Cells

Frontiers in Oncology, 2022
BackgroundTriple-negative breast cancer (TNBC) is relatively common in women and is associated with a poor prognosis after surgery and adjuvant chemotherapy.
Chen Sun, Chen Sun, Pan Liu, Pan Liu, Pan Liu, Lijian Pei, Lijian Pei, Lijian Pei, Mengyun Zhao, Mengyun Zhao, Yuguang Huang, Yuguang Huang   +11 more
doaj   +2 more sources

Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS

Journal of the American Chemical Society, 2012
This study examines the dynamic co-localization of lipid-anchored fluorescent proteins in living cells using pulsed-interleaved excitation fluorescence cross-correlation spectroscopy (PIE-FCCS) and fluorescence lifetime analysis. Specifically, we look at the pairwise co-localization of anchors from lymphocyte cell kinase (LCK: myristoyl, palmitoyl ...
Triffo, Sara B., Huang, Hector H., Smith, Adam W., Chou, Eldon T., Groves, Jay T.   +4 more
openaire   +3 more sources