Results 1 to 10 of about 57,803 (192)

PieParty: visualizing cells from scRNA-seq data as pie charts [PDF]

Life Science Alliance, 2021
Single-cell RNA sequencing (scRNA-seq) has been a transformative technology in many research fields. Dimensional reduction techniques such as UMAP and tSNE are used to visualize scRNA-seq data in two or three dimensions for cells to be clustered in biologically meaningful ways.
Stefan Kurtenbach, James J Dollar, Anthony M Cruz, Michael A Durante, Christina L Decatur, J William Harbour   +5 more
openaire   +3 more sources

PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells [PDF]

Biophysical Journal, 2020
ABSTRACT We report the use of pulsed interleaved excitation-fluorescence lifetime imaging microscopy (PIE-FLIM) to measure the activities of two different biosensor probes simultaneously in single living cells. Many genetically encoded biosensors rely on the measurement of Förster resonance energy transfer (FRET) to detect changes in ...
Reissaus, Christopher A., Day, Kathleen H., Mirmira, Raghavendra G., Dunn, Kenneth W., Pavalko, Fredrick M., Day, Richard N.   +5 more
openaire   +3 more sources

A dendrite in every pie [PDF]

Virulence, 2012
Dendritic cells (DC) are a heterogeneous population of innate immune cells that are fundamental to initiating responses against invading pathogens and regulating immune responses. Myeloid DC (mDC) act as a bridge between the innate and adaptive immune response during virus infections but their role in immunity to human immunodeficiency virus (HIV ...
Wonderlich, Elizabeth R., Barratt-Boyes, Simon M.   +1 more
openaire   +2 more sources

Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS

Journal of the American Chemical Society, 2012
This study examines the dynamic co-localization of lipid-anchored fluorescent proteins in living cells using pulsed-interleaved excitation fluorescence cross-correlation spectroscopy (PIE-FCCS) and fluorescence lifetime analysis. Specifically, we look at the pairwise co-localization of anchors from lymphocyte cell kinase (LCK: myristoyl, palmitoyl ...
Triffo, Sara B., Huang, Hector H., Smith, Adam W., Chou, Eldon T., Groves, Jay T.   +4 more
openaire   +2 more sources

Connecting PIE-FCCS Measurements to Dimerization Affinity to Resolve the Role of GPCR Dimerization in Live Cells [PDF]

Biophysical Journal, 2016
Since the rhodopsin crystal structure was reported over 15 years ago, the structure and dynamics of class A G-protein coupled receptors (GPCR) have been a growing area of investigation. A significant body of research has shown the existence of GPCR dimers and possibly higher order oligomers in native tissue.
openaire   +1 more source

Eosinophilic pericarditis with atypical plasma cells in PIE syndrome.

The Journal of the Japanese Society of Clinical Cytology, 1987
Tamiko KIMURA, Akinori ISHIHARA, Akira KAMIMORI, Hideyuki KOYAMA, Mitsuya NOGUCHI, Hiroshi KOMADA, Ryuichi YATANI   +6 more
openaire   +2 more sources

GROUPES SANGUINS ET SITUATION GÉNÉTIQUE DE LA RACE BOVINE FLAMANDE. VARIABILITÉ GÉNÉTIQUE COMPARÉE A CELLE DE LA RACE « FRANÇAISE FRISONNE PIE-NOIRE» ; PARENTÉ AVEC LES RACES « DANOISE ROUGE » ET « BELGE DE FLANDRE OCCIDENTALE » [PDF]

Annales de Biologie Animale Biochimie Biophysique, 1968
Bouquet, Y., Grosclaude, F., Bezard, G., Houlier, G., Ruffet, G., Troccon, Anne   +5 more
openaire   +2 more sources

Lipidome Analysis of Cancer Cells and Their Extracellular Vesicles Reveals Cancer-Type-Specific Lipid Signatures and Enables the Design of EV-Mimetic Liposomes. [PDF]

J Extracell Vesicles
Douanne N, Benslimane Y, López RR, Bustamante P, Tsering T, Chen Y, Kienzle L, Ghasemi M, Taylor L, Chen Y, Boutin AT, Juncker D, Mounier C, Nerguizian V, Burnier JV.   +14 more
europepmc   +1 more source

Tumor immune microenvironment score predicts efficacy of immune checkpoint inhibitors-based regimens in advanced non-small cell lung cancer. [PDF]

J Transl Med
Dai J, Yan H, Chen Y, Zhang Y, Huang Z, Ruan Z, Tian F, Qin H, Xu Q, Wang J, Li X, Cheng P, Zhu C, Yang N, Zeng L, Zhang Y.   +15 more
europepmc   +1 more source

Differentiation of sphingomyelin and cholesterol by hyperspectral mid-infrared detection of single-bond vibrational modes in the fingerprint region. [PDF]

Nat Methods
Gasparin F, Prebeck A, Soldà A, Uluç N, Glasl S, Berger C, Pleitez MA, Ntziachristos V.   +7 more
europepmc   +1 more source