Results 121 to 130 of about 16,481,877 (361)

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Functional imaging of plants: A nuclear magnetic resonance study of a cucumber plant

, 2002
Functional magnetic resonance imaging was used to study transients of biophysical parameters in a cucumber plant in response to environmental changes. Detailed flow imaging experiments showed the location of xylem and phloem in the stem and the response ...
As, H., van, Scheenen, T., Heemskerk, A., Vergeldt, F.J., Jager, A., de   +4 more
core   +1 more source

The Ralstonia solanacearum Type III Effector RipAY Is Phosphorylated in Plant Cells to Modulate Its Enzymatic Activity

Frontiers in Plant Science, 2017
Most bacterial pathogens subvert plant cellular functions using effector proteins delivered inside plant cells. In the plant pathogen Ralstonia solanacearum, several of these effectors contain domains with predicted enzymatic activities, including ...
Yali Wei, Yali Wei, Yuying Sang, Alberto P. Macho   +3 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Preliminary assessment of the effect of plant protease inhibitors on the expression of heterologous genes in plant cells / Nassim Khalighi [PDF]

, 2012
Despite the successful development of pant-based expression systems for the production of a wide range of recombinant proteins in recent years, there remain serious limitations in the final yield accumulation of the foreign protein in the plant cells ...
Khalighi, Nassim
core  

Trafficking of phosphatidylinositol 3-phosphate from the trans-Golgi network to the lumen of the central vacuole in plant cells

, 2019
Very limited information is available on the role of phosphatidytinositol 3-phosphate (P1[3]P) in vesicle trafficking in plant cells. To investigate the role of PI(3)P during the vesicle trafficking in plant cells, we exploited the Pt(3)P-specific ...
Pih, KT, Yoo, CM, Kim, DH, Kim, YW, Eu, YJ, Jin, JB, Hwang, I, Kim, SJ, Stenmark, H   +8 more
core   +1 more source

Systems dynamic modeling of a guard cell Cl- channel mutant uncovers an emergent homeostatic network regulating stomatal transpiration [PDF]

, 2012
Stomata account for much of the 70% of global water usage associated with agriculture and have a profound impact on the water and carbon cycles of the world.
Yizhou Wang, Adrian Hills, Lew, V.L., Mary Williams, Hills, A., Virgilio L. Lew, Eisenach, C., Papanatsiou, M., Michael R. Blatt, Williams, M., Karnik, R., Maria Papanatsiou, Cornelia Eisenach, Rucha Karnik, Wang, Y., Blatt, M.R.   +15 more
core   +1 more source

Chemical Interactions at the Interface of Plant Root Hair Cells and Intracellular Bacteria

, 2021
In this research, we conducted histochemical, inhibitor and other experiments to evaluate the chemical interactions between intracellular bacteria and plant cells.
Kathryn Kingsley, James White, Xiaoqian Chang   +2 more
core   +1 more source

Aging restricts the initial neural patterning potential of developing neural stem and progenitor cells in the adult brain

Frontiers in Aging Neuroscience
IntroductionNeurosphere culture is widely used to expand neural stem and progenitor cells (NSPCs) of the nervous system. Understanding the identity of NSPCs, such as the principals involved in spatiotemporal patterning, will improve our chances of using ...
Saeideh Aran, Mohammad Ghasem Golmohammadi, Mohsen Sagha, Kamran Ghaedi   +3 more
doaj   +1 more source