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Protoheme extraction from plant tissue

Analytical Biochemistry, 1978
A method for reproducibly estimating the protoheme content of plant tissues has been developed. The tissue sample is homogenized in 80% acetone to remove pigments and lipids; protoheme is then extracted from the tissue residue with 2% HCl in acetone and quantitatively transferred into diethyl ether.
L C, Stillman, M L, Gassman
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Metabolomic fingerprinting of plant extracts

Journal of Mass Spectrometry, 2006
AbstractThe standardization and quality control of plant extracts is an important topic, in particular, when such extracts are used for medicinal purposes. Consequently, the development of fast and effective analytical methods for metabolomic fingerprinting of plant extracts is of high interest.
MATTOLI L   +8 more
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Membrane Extracts from Plant Tissues

2020
The comparison of isolated plant cell membranous enclosures can be hampered if their extraction method differs, e.g., in regard to the utilized buffers, the tissue, or the developmental stage of the plant. Thus, for comparable results, different cellular compartments should be isolated synchronously in one procedure.
Uwe, Bodensohn   +3 more
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Plant extracts in BPH.

Minerva urologica e nefrologica = The Italian journal of urology and nephrology, 1993
In Italy plant extracts represent 8.6% of all pharmacological prescriptions for Benign Prostatic Hyperplasia (data from 1991). This review evaluates all the suggested mechanisms of action for plant extracts. Recently we demonstrated an antiestrogenic effect of Serenoa Repens in BPH patients.
DI SILVERIO, Franco   +7 more
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Plant Tissue Extraction for Metabolomics

2013
Plants are not only important producers of foods and energy storages (e.g., sugars, carbohydrates, proteins, and fats) in the form of grains, fruits, and vegetables, they also provide many valuable products to human existence including wood, fibers, oils, resins, pigments, antioxidants, and sources of medicine.
Ute, Roessner, Daniel Anthony, Dias
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Telomerase activity in plant extracts

Molecular and General Genetics MGG, 1996
Telomeres of most eucaryotes terminate in long stretches of short, guanine-rich repeats. Telomerase, a specialized enzyme with reverse transcriptase-like activity, has been shown to synthesize these repeats in many lower eucaryotes and several animal species.
K, Heller   +3 more
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Extraction of Plant Secondary Metabolites

2006
This chapter presents an overview of the preparation of extracts from plants using organic solvents, with emphasis on common problems encountered and methods for their reduction or elimination. In addition to generally applicable extraction protocols, methods are suggested for selectively extracting specific classes of plant-derived compounds, and ...
William P, Jones, A Douglas, Kinghorn
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Plant DNA extraction.

2001
The extraction of DNA from plants is the starting point for genotype analysis. The approach to preparation of DNA from plants is determined by the species, the type of tissue or sample available and the analysis required on the DNA. For example, extraction of DNA from the leaf of a cereal for analysis using a robust test may require a very different ...
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Antioxidative plant extracts for chemoprevention

Archives of Toxicology, 2013
model certainly demonstrates a strong antioxidative activity. However, it remains to be shown whether efficiency under such drastic conditions will also be protective under the much milder conditions seem in human liver diseases (Godoy et2013 al. ). Similar to the liver, several in vivo studies have demonstrated antioxida-tive effects in the central ...
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Protein Extraction from Woody Plants

2006
In this chapter we present a protocol for total protein extraction optimized for wood-forming tissue (differentiating secondary xylem). The protocol is then used for a series of other organs (root, leaf, pollen, bud, flower, cambium, and phloem) in broadleaf (oak and poplar) and conifer (pine) species.
Plomion, Christophe, Lalanne, Céline
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