Results 91 to 100 of about 145,446 (253)

A Novel Method to Titrate Herpes Simplex Virus-1 (HSV-1) Using Laser-Based Scanning of Near-Infrared Fluorophores Conjugated Antibodies

Frontiers in Microbiology, 2017
Among several strategies used for Herpes simplex virus (HSV) detection in biological specimens, standard plaque assay (SPA) remains the most reliable method to evaluate virus infectivity and quantify viral replication.
Marco Fabiani, Dolores Limongi, Anna Teresa Palamara, Anna Teresa Palamara, Giovanna De Chiara, Maria Elena Marcocci   +5 more
doaj   +1 more source

A Dual‐Phase Exosomal Nanotherapy Enhances Continual Efferocytosis by Coordinating Checkpoint Inhibition and Metabolic Reprogramming in Atherosclerosis

Advanced Science, EarlyView.
Here we developed Am@SExo, a dual‐functional engineered exosome that coordinates CD47–SIRPα checkpoint blockade with Arg1 mRNA–mediated metabolic reprogramming. Surface SIRPα promotes recognition of apoptotic cells and plaque targeting, while Arg1 expression enhances arginine–ornithine metabolism, Rac1 activation, and actin remodeling in macrophages ...
Danwen Zheng, Shiteng Cai, Jinfeng Gao, Sheng Zhao, Bohan Wei, Xueyi Weng, Zhengmin Wang, Qiaozi Wang, Qiyu Li, Chengzhi Han, Weiyan Li, Yiwen Tan, Yuyuan Fu, Meng Ji, Zheyong Huang, Yanan Song, Juying Qian, Junbo Ge   +17 more
wiley   +1 more source

Metaviromes Reveal the Dynamics of Pseudomonas Host-Specific Phages Cultured and Uncultured by Plaque Assay. [PDF]

Viruses, 2021
Alanin KWS, Junco LMF, Jørgensen JB, Nielsen TK, Rasmussen MA, Kot W, Hansen LH.   +6 more
europepmc   +1 more source

Machine Learning‐Enhanced Ultrasensitive Immuno‐CRISPR Array Facilitates Early Diagnosis of Alzheimer's Disease by Detecting Multiple Plasma Biomarkers

Advanced Science, EarlyView.
This work presents a CRISPR‐based, ultrasensitive multiplex protein detection array capable of simultaneously analyzing six plasma biomarkers associated with Alzheimer's disease (AD). By integrating antibody‐mediated signal transduction, recombinase polymerase amplification, and spatially encoded CRISPR‐Cas12a, the system achieves detection sensitivity
Liding Zhang, Changwen Yang, Qian Yao, Xuewei Du, Shuai Ding, Yaoqiang Shi, Can Sheng, Ming Wang, Ying Han, Haiming Luo   +9 more
wiley   +1 more source

Simple and rapid plaque assay for recombinant baculoviruses expressing influenza hemagglutinin. [PDF]

Sci Prog, 2021
Basak S, Kang HJ, Chu KB, Oh J, Quan FS.
europepmc   +1 more source

Microplastics‐Induced Gut Microbiota Dysbiosis Accelerates Alzheimer's‐Like Pathology and Cognitive Decline via the Gut–Brain Axis

Advanced Science, EarlyView.
Microplastics (MPs) breach the gut‐brain axis by triggering taurine‐depleting microbiota dysbiosis that accelerates Alzheimer‐like pathology in 5XFAD mice. Taurine supplementation normalizes autophagy, microglial quiescence, and cognition, and plasma taurine inversely tracks cognitive decline in ADNI participants, revealing a readily translatable ...
Zifeng Wu, Jiarong Yang, Miaoxuan Zhang, Yi Li, Zhuling Tu, Ji Wang, Jin‐Tai Yu, Kun Guo, Rongcan Luo   +8 more
wiley   +1 more source

Cytopathic Effect Assay and Plaque Assay to Evaluate in vitro Activity of Antiviral Compounds Against Human Coronaviruses 229E, OC43, and NL63. [PDF]

Bio Protoc, 2022
Hu Y, Ma C, Wang J.
europepmc   +1 more source

Observations on the Plaque Assay of Visna Virus

Journal of General Virology, 1969
Visna virus is the cause of a slowly evolving neurological illness of sheep (Sigurdsson, Palsson & Grimsson, 1957; Sigurdsson & Palsson, 1958). The virus has been propagated in cell cultures derived from sheep choroid plexus and quantified by 50% endpoint determinations of cytopathic effects in sheep choroid plexus cells 14 to 21 days after inoculation
openaire   +2 more sources

p300 Degradation by the p53‐SIAH1 Axis Relieves TBK1 Acetylation to Enhance Innate Antiviral Immunity

Advanced Science, EarlyView.
This study identifies p300 as the acetyltransferase that acetylates TBK1 and inhibits its phosphorylation. Activation of the p53‐SIAH1 axis by immune response downregulates p300 expression to sustain innate antiviral immunity. Conditional p300 knockout in alveolar epithelial cells in vivo promotes antiviral responses and suppresses virus replication ...
Huidi Yu, Zhihao Zhan, Xiaoxiang Pan, Xinyue Zhang, Penggang Liu, Jing Sun, Xiulong Xu   +6 more
wiley   +1 more source

Comparison among plaque assay, tissue culture infectious dose (TCID₅₀) and real-time RT-PCR for SARS-CoV-2 variants quantification. [PDF]

Iran J Microbiol, 2022
Zapata-Cardona MI, Flórez-Álvarez L, Gómez-Gallego DM, Moncada-Díaz MJ, Hernandez JC, Díaz F, Rugeles MT, Aguilar-Jiménez W, Zapata W.   +8 more
europepmc   +1 more source