Results 101 to 110 of about 152,477 (295)

Orthogonal Ionic Liquid‐Based Extraction Strategy Enables Amyloid‐Specific Profiling of Aggregate Proteome

Advanced Science, EarlyView.
An orthogonal ionic‐liquid extraction (Orth‐iEA) enables selective isolation of amyloid fibrils. TMGBF4 disrupts hydrogen‐bonded β‐sheet networks to solubilize amyloid aggregates, whereas C12ImCl interacts with hydrophobic regions of non‐amyloid proteins.
Shiying Zheng, Ye Liu, Bowen Zhong, Huiying Chu, Zhou Gong, Baofeng Zhao, Mengchun Cheng, Zhen Liang, Yukui Zhang, Qun Zhao, Lihua Zhang   +10 more
wiley   +1 more source

Activity of anti-H3 mabs in plaque reduction assay on MDCK cells.

, 2013
mAb 7A7 (A), 12D1 (B) and 39A4 (C) neutralize all H3 viruses tested by plaque reduction assay but not representative H1, H4 or H7 viruses. Purified mouse IgG was used for the negative control. The plaque reduction assays were performed multiple times and
Peter Palese (59796), Gene S. Tan (310399), Erin Petersen (366748), Natalie Pica (310400), Taia T. Wang (366747), Rong Hai (186437), Thomas M. Moran (198068)   +6 more
core   +1 more source

An improved respiratory syncytial virus neutralization assay based on the detection of green fluorescent protein expression and automated plaque counting [PDF]

, 2012
Background Virus neutralizing antibodies against respiratory syncytial virus (RSV) are considered important correlates of protection for vaccine evaluation.
Huisman Willem, van Eldik Mandy, Mandy van Eldik, Xu Fang, Myra N Widjojoatmodjo, Widjojoatmodjo Myra N, Yvonne van Remmerden, Jacco GM Heldens, van Remmerden Yvonne, Fang Xu, Heldens Jacco GM, Willem Huisman   +11 more
core   +1 more source

A Plaque Assay for Feline Panleukopenia Virus

Journal of General Virology, 1980
Plaque formation with representative strains of feline panleukopenia virus (FPV) has been obtained using a permanent line of feline kidney cells under agarose overlay. FPV-infected cells appear as white plaques after neutral red staining. Plaque size is determined by the extent of cell division in the infected monolayer.
G, Siegl, G, Kronauer
openaire   +2 more sources

Divergent Roles of mGlu2 and mGlu3 Receptors in Amyloid‐β Production and Cognitive Dysfunctions in Alzheimer's Disease

Advanced Science, EarlyView.
This study explores the opposing effects of the mGluR2 and mGluR3 receptors on amyloid precursor protein processing. mGluR2 promotes amyloidogenic cleavage, while mGluR3 favors non‐amyloidogenic pathways. Using a brain‐penetrant nanobody as a mGluR2 positive allosteric modulator, the study uncovers how its chronic activation aggravates amyloid‐β burden
Pierre‐André Lafon, Mireille Elodie Tsitokana, Ugo Guy Alenda, Yen‐Ling Lian, Clémentine Eva Philibert, Mathieu Oosterlaken, Marta Cimadevila, Gaëlle Dudon, Jessica Monnic, Salomé Roux, Julie Bessié, Séverine Diem, Franck Vandermoere, Laurent Prézeau, Patrick Chames, Julie Kniazeff, Sylvie Claeysen, Anaïs Menny, Jean‐Philippe Pin, Véronique Perrier, Jianfeng Liu, Philippe Rondard   +21 more
wiley   +1 more source

Ecdysteroid Secreting Cells Indentified by Reverse Haemolytic Plaque Assay

, 2011
Ecdysteroide spielen eine entscheidende Rolle in der hormonellen Regulation der Entwicklung von Arthropoden. Sie werden von einer spezialisierten Hormondrüse, der so genannten Prothorakaldrüse, sezerniert.
Duckwitz, Hauke
core   +1 more source

Oral Motion‐Powered Smart Dental Implant Abutment for In Situ Antibacterial and Cell Adhesion Through Piezoelectric Effect

Advanced Science, EarlyView.
We proposed a method based on the piezoelectric effect and fabricated an implant abutment on the basis of piezoelectric ceramic. Under physiological occlusal pressure, the oral motion‐powered smart dental implant abutments interacted with water and oxygen, promoting the formation of reactive oxygen species and achieving a synergistic and highly ...
Xiyu Shi, Xiaoyu Han, Yanhui Lu, Yuan Chai, Baiyan Xiao, Shuo Liu, Boon Chin Heng, Tingting Wu, Tingjun Li, Qiaomei Ren, Ting Song, Le Chen, Dong Han, Yaru Guo, Xuliang Deng, Xuehui Zhang   +15 more
wiley   +1 more source

A Standardized Plaque Reduction Assay for Determination of Drug Susceptibilities of Cytomegalovirus Clinical Isolates

, 2000
Twelve laboratories collaborated in formulating and testing a standardized plaque reduction assay for cytomegalovirus (CMV) cell-associated clinical isolates.
Gilliam, B., Crumpacker, C., Britt, W., Erice, A., Yen-Lieberman, B., Brambilla, D., Landry, M. L., Biron, K., Manischewitz, J., Lurain, N., Nokta, M., Spector, S., Drew, W. L., Jokela, J., Miner, R., Reichelderfer, P., Weinberg, A., Stanat, S., Chou, S.   +18 more
core   +1 more source

Development of a robust cell-based potency assay for a coxsackievirus A21 oncolytic virotherapy

Heliyon
Oncolytic viruses (OV) are part of a burgeoning field of investigational oncolytic therapy (OT), in which lytic viruses dissolve advanced tumors productively and specifically.
Venkateswarlu Chamcha, Li He, Jenny Xu, Andrew R. Swartz, Erin Green-Trexler, Kevin Gurney, Tessie McNeely   +6 more
doaj   +1 more source

Lilrb4a Suppression Reprograms Microglia to Mitigate APOE4‐Associated Amyloid Plaques and Cerebral Amyloid Angiopathy in Association With a PPAR‐Linked Pro‐Clearance State

Advanced Science, EarlyView.
Targeting Lilrb4a in Apolipoprotein E4 (APOE4)‐associated Alzheimer's disease (AD) reprograms microglia toward a beneficial, phagocytic state. Genetic deletion or antisense inhibition of Lilrb4a suppresses p‐SHP2/NF‐κB/STAT1 signaling, restores PPAR‐linked lipid and energy metabolism, and reduces amyloid plaque burden and cerebral amyloid angiopathy ...
Changxu Nie, Ruixi Yang, Xiaotong Wang, Ping Jia, Xueqi Zhang, Yaqi Dai, Xue Bai, Sijia Duan, Yufeng Li, Peng Zheng, Xin Tian, Li Jiang, Chao Wang   +12 more
wiley   +1 more source