Evaluation of a commercial ELISA as alternative to plaque reduction neutralization test to detect neutralizing antibodies against SARS-CoV-2 [PDF]
Scientific Reports, 2022 High-throughput detection of neutralizing antibodies against SARS-CoV-2 presents a valuable tool for vaccine trials or investigations of population immunity.
Natalie Hofmann +4 more
doaj +8 more sources
Plaque Reduction Neutralization Test (PRNT) Accuracy in Evaluating Humoral Immune Response to SARS-CoV-2 [PDF]
DiseasesMassive vaccination positively impacted the SARS-CoV-2 pandemic, being a strategy to increase the titers of neutralizing antibodies (NAbs) in the population.
Ingrid Siciliano Horbach +23 more
doaj +6 more sources
Pseudo-plaque reduction neutralization test (PPRNT) for the measurement of neutralizing antibodies to Crimean-Congo hemorrhagic fever virus [PDF]
Virology Journal, 2013 Background Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus of the genus Nairovirus family Bunyaviridae, which are enveloped viruses containing tripartite, negative polarity, single-stranded RNA.
Canakoglu Nurettin +8 more
doaj +9 more sources
Validation and Establishment of the SARS-CoV-2 Lentivirus Surrogate Neutralization Assay as a Prescreening Tool for the Plaque Reduction Neutralization Test [PDF]
Microbiology Spectrum, 2023 Neutralization assays are important for understanding and quantifying neutralizing antibody responses toward severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
John Merluza +10 more
doaj +4 more sources
Evaluation of a SARS-CoV-2 lateral flow assay using the plaque reduction neutralization test. [PDF]
Diagn Microbiol Infect Dis, 2021 As new tests and technologies advance our understanding and diagnostic capabilities of the severe acute respiratory syndrome coronavirus 2 and the coronavirus disease 2019, they must be appropriately validated to make sure test performance is following manufacturer claims. In this study, we evaluated the Vazyme 2019-nCoV IgG/IgM Detection Kit, which is
Xie X +4 more
europepmc +5 more sources
Evaluating Humoral Immunity against SARS-CoV-2: Validation of a Plaque-Reduction Neutralization Test and a Multilaboratory Comparison of Conventional and Surrogate Neutralization Assays [PDF]
Microbiology Spectrum, 2021 The evaluation of humoral protective immunity against SARS-CoV-2 remains crucial in understanding both natural immunity and protective immunity conferred by the several vaccines implemented in the fight against COVID-19.
Emelissa J. Valcourt +30 more
doaj +3 more sources
Comparison of JEV neutralization assay using pseudotyped JEV with the conventional plaque-reduction neutralization test. [PDF]
J Microbiol, 2014 We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV-PV). JEV-PV-based neutralization assay offers several advantages compared with the current standard plaque-reduction neutralization test (PRNT), including simplicity, safety, and
Lee HJ +7 more
europepmc +6 more sources
Plaque Reduction Neutralization Test (PRNT) in the Congenital Zika Syndrome: Positivity and Associations with Laboratory, Clinical, and Imaging Characteristics [PDF]
Viruses, 2020 The short duration of viremia, low blood viral load, inaccessibility to timely specific diagnostic tests, and cross-reactions with other flaviviruses have hindered laboratory confirmation of Congenital Zika Syndrome (CZS).
Marizélia R. C. Ribeiro +16 more
doaj +4 more sources
Varicella-zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique [PDF]
Journal of Clinical Microbiology, 1976 A new plaque assay for the quantitation of varicella-zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed using the indirect immunoperoxidase technique. As compared with the classical plaque assay using a solid overlay, the test gives earlier results since plaque counting can ...
Giuseppe Gerna, Rachel Chambers
openalex +4 more sources
Viruses, 2023 Since their first documentation in 1952, plaque reduction neutralization tests (PRNTs) have become the choice of test for the measurement of neutralizing antibodies against a particular virus.
Sunil R. Vaidya
doaj +3 more sources