Results 21 to 30 of about 16,802 (217)
A Plaque Reduction Neutralization Test for Human Cytomegalovirus.
Experimental Biology and Medicine, 1964 SummaryA plaque technique for human cytomegaloviruses has been developed employing human embryonic lung fibroblast cultures and an overlay containing 2% methyl cellulose. Neutralization tests employing the 80% plaque reduction endpoint were found to give reproducible results. Human adult sera exhibited titers of 1:8 to 1:64.
G, PLUMMER, M, BENYESH-MELNICK
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Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization [PDF]
Journal of Clinical Microbiology, 1985 A newly modified semimicro plaque reduction neutralization test (PRNT) in BHK cells was compared with a standard PRNT in bottles with LLC-MK2 monolayers and with an LLC-MK2 PRNT adapted to semimicro methods. The BHK semimicro PRNT compared favorably in terms of sensitivity in detecting dengue antibody (96%), specificity at a screening dilution (95 ...
D M, Morens +4 more
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An Integrated Research–Clinical BSL-2 Platform for a Live SARS-CoV-2 Neutralization Assay
Viruses, 2023 A reliable and efficient serological test is crucial for monitoring neutralizing antibodies against SARS-CoV-2 and its variants of concern (VOCs). Here, we present an integrated research–clinical platform for a live SARS-CoV-2 neutralization assay ...
Jing Zou +14 more
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Fluorescence Adherence Inhibition Assay: A Novel Functional Assessment of Blocking Virus Attachment by Vaccine-Induced Antibodies. [PDF]
PLoS ONE, 2016 Neutralizing antibodies induced by vaccination or natural infection play a critically important role in protection against the viral diseases. In general, neutralization of the viral infection occurs via two major pathways: pre- and post-attachment modes,
Atul Asati +9 more
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Onderstepoort Journal of Veterinary Research, 2004 Blood samples from 50 dogs were collected at three veterinary clinics in Ibadan and Abuja, Nigeria and the serum from each sample was evaluated serologically for neutralizing antibodies against canine distemper virus (CDV) by the highly sensitive ...
O.I. Oyedele +3 more
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Serotypic classification of hantaviruses by indirect immunofluorescent antibody and plaque reduction neutralization tests [PDF]
Journal of Clinical Microbiology, 1985 Antisera prepared against 16 strains of hantaviruses isolated from patients with hemorrhagic fever with renal syndrome (HFRS) or from rodents captured in HFRS-endemic and nonendemic regions were titrated against Hantaan virus strain 76-118, Puumala virus strain Sotkamo, and Prospect Hill virus strain Prospect Hill-I by using the indirect ...
P W, Lee +3 more
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Animals, 2022 Coronavirus disease (COVID-19) is an emerging infectious disease caused by SARS-CoV-2. Given the emergence of SARS-CoV-2 variants, continuous surveillance of SARS-CoV-2 in animals is important.
Nareerat Sangkachai +13 more
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Variation in dengue virus plaque reduction neutralization testing: systematic review and pooled analysis [PDF]
BMC Infectious Diseases, 2012 AbstractBackgroundThe plaque reduction neutralization test (PRNT) remains the gold standard for the detection of serologic immune responses to dengue virus (DENV). While the basic concept of the PRNT remains constant, this test has evolved in multiple laboratories, introducing variation in materials and methods.
Rainwater-Lovett, Kaitlin +3 more
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Serologic Evidence of West Nile Virus Transmission, Jamaica, West Indies
Emerging Infectious Diseases, 2003 In spring 2002, an intensive avian serosurvey was initiated in Jamaica, Puerto Rico, and Mexico. We collected >1,600 specimens from resident and nonresident neotropical migratory birds before their northerly migrations.
Alan P. Dupuis +2 more
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