Results 41 to 50 of about 16,802 (217)

A real-time and high-throughput neutralization test based on SARS-CoV-2 pseudovirus containing monomeric infrared fluorescent protein as reporter

Emerging Microbes and Infections, 2021
Neutralizing antibodies to SARS-CoV-2 have been shown to correlate with protection in animals and humans, disease severity, survival, and vaccine efficacy.
Wen-Yang Tsai, Lauren L. Ching, Szu-Chia Hsieh, Marian E. Melish, Vivek R. Nerurkar, Wei-Kung Wang   +5 more
doaj   +1 more source

Evaluation of Chimeric Japanese Encephalitis and Dengue Viruses for Use in Diagnostic Plaque Reduction Neutralization Tests [PDF]

Clinical and Vaccine Immunology, 2009
ABSTRACTThe plaque reduction neutralization test (PRNT) is a specific serological test used to identify and confirm arbovirus infection in diagnostic laboratories and monitor immunological protection in vaccine recipients. Wild-type (wt) viruses used in the PRNT may be difficult to grow and plaque titrate, such as the dengue viruses (DENV), and/or may ...
Barbara W, Johnson, Olga, Kosoy, Elizabeth, Hunsperger, Manuela, Beltran, Mark, Delorey, Farshad, Guirakhoo, Thomas, Monath   +6 more
openaire   +2 more sources

Evaluation of Neutralizing Activity against Omicron Subvariants in BA.5 Breakthrough Infection and Three-Dose Vaccination Using a Novel Chemiluminescence-Based, Virus-Mediated Cytopathic Assay

Microbiology Spectrum, 2023
Neutralizing potency of humoral immune responses induced by prior infection or vaccination is vital for protecting of individuals and population against severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2). However, the emergence of viral
Mako Toyoda, Toong Seng Tan, Chihiro Motozono, Godfrey Barabona, Akiko Yonekawa, Nobuyuki Shimono, Rumi Minami, Yoji Nagasaki, Yusuke Miyashita, Hiroyuki Oshiumi, Kimitoshi Nakamura, Shuzo Matsushita, Takeo Kuwata, Takamasa Ueno   +13 more
doaj   +1 more source

A Dual‐Phase Exosomal Nanotherapy Enhances Continual Efferocytosis by Coordinating Checkpoint Inhibition and Metabolic Reprogramming in Atherosclerosis

Advanced Science, EarlyView.
Here we developed Am@SExo, a dual‐functional engineered exosome that coordinates CD47–SIRPα checkpoint blockade with Arg1 mRNA–mediated metabolic reprogramming. Surface SIRPα promotes recognition of apoptotic cells and plaque targeting, while Arg1 expression enhances arginine–ornithine metabolism, Rac1 activation, and actin remodeling in macrophages ...
Danwen Zheng, Shiteng Cai, Jinfeng Gao, Sheng Zhao, Bohan Wei, Xueyi Weng, Zhengmin Wang, Qiaozi Wang, Qiyu Li, Chengzhi Han, Weiyan Li, Yiwen Tan, Yuyuan Fu, Meng Ji, Zheyong Huang, Yanan Song, Juying Qian, Junbo Ge   +17 more
wiley   +1 more source

Microplastics‐Induced Gut Microbiota Dysbiosis Accelerates Alzheimer's‐Like Pathology and Cognitive Decline via the Gut–Brain Axis

Advanced Science, EarlyView.
Microplastics (MPs) breach the gut‐brain axis by triggering taurine‐depleting microbiota dysbiosis that accelerates Alzheimer‐like pathology in 5XFAD mice. Taurine supplementation normalizes autophagy, microglial quiescence, and cognition, and plasma taurine inversely tracks cognitive decline in ADNI participants, revealing a readily translatable ...
Zifeng Wu, Jiarong Yang, Miaoxuan Zhang, Yi Li, Zhuling Tu, Ji Wang, Jin‐Tai Yu, Kun Guo, Rongcan Luo   +8 more
wiley   +1 more source

Evaluation of three alternative methods to the plaque reduction neutralizing assay for measuring neutralizing antibodies to dengue virus serotype 2

Virology Journal
Background Dengue is a global public health challenge which requires accurate diagnostic methods for surveillance and control. The gold standard for detecting dengue neutralizing antibodies (nAbs) is the plaque reduction neutralization test (PRNT), which
Vanessa Shi Li Goh, Christopher Chong Wei Ang, Swee Ling Low, Pei Xuan Lee, Yin Xiang Setoh, Judith Chui Ching Wong   +5 more
doaj   +1 more source

Advances in Detection Methods for Human Respiratory Syncytial Virus Neutralizing Antibodies

Vaccines
Human respiratory syncytial virus (HRSV) is a major cause of severe lower respiratory tract infections in infants, young children, and older adults worldwide.
Qi Shen, Jing Gai, Yanqiu Zhou
doaj   +1 more source

Slc44a2 Deficiency Unveils an IFN‐I–Dependent Feedback Control of pDC Egress

Advanced Science, EarlyView.
Working model of SLC44A2‐mediated maintenance of pDC homeostasis. This model illustrates two central mechanisms by which SLC44A2 regulates pDC homeostasis: (1) SLC44A2 limits IFN‐I production by exporting amino acids (T, N, Q), thereby preventing spontaneous pDC activation.
Ruiqun Chen, Tao Wu, Zhen Shi, Ravi Kumar Verma, Linlin Sheng, Lei Tao, Mingming Yang, Yuchen Wang, Conggang Zhang, Hao Fan, Li Wu, Ligong Chen   +11 more
wiley   +1 more source

Diagnosis of arboviruses using indirect sandwich IgG ELISA in horses from the Brazilian Amazon

Journal of Venomous Animals and Toxins including Tropical Diseases, 2014
Background The Amazon as a whole is the largest reservoir of arboviruses worldwide, while the Brazilian Amazon hosts the largest variety of arboviruses isolated to date.
Alexandre do Rosário Casseb, Márcio Roberto Teixeira Nunes, Sueli Guerreiro Rodrigues, Elizabeth Salbé Travassos da Rosa, Livia Medeiros Neves Casseb, Samir Manssor Moraes Casseb, Sandro Patroca da Silva, Érika Dayane Leal Rodrigues, Pedro Fernando da Costa Vasconcelos   +8 more
doaj   +1 more source

Overcoming Transmission Barriers: A Dual‐Functional Adhesive Hydrogel for Direct Monkeypox Virus Neutralization and Wound Healing

Advanced Science, EarlyView.
MPXV infection leads to persistent viral replication and severe local inflammation, while existing treatments cannot simultaneously remove viruses and repair injured tissue. This study constructs Polymer@mPDA@Gel hydrogel via a two‐step hierarchical assembly method: mPDA anchors photothermal polymers to form antiviral photothermal subunits, which are ...
Ming Zhang, Jiaxin Tian, Xiaolong Zhu, Xiao Li, Xiangshu Qiu, Yuanyuan Li, Ben Zhong Tang, Ning Shi   +7 more
wiley   +1 more source