Plaque reduction viral neutralization by anti-M2e antibodies.
The neutralization capability of sera obtained from chickens vaccinated with PBS (mock), M2eP-2x (anti-M2e), and H7N2 (anti-H7N2) or H5N2 (anti-H5N2) immune sera was tested against H7N2 (A) and H5N2 (B) viruses.
Jagadish Hiremath (529768) +11 more
core +1 more source
Evaluation of Chimeric Japanese Encephalitis and Dengue Viruses for Use in Diagnostic Plaque Reduction Neutralization Tests [PDF]
ABSTRACTThe plaque reduction neutralization test (PRNT) is a specific serological test used to identify and confirm arbovirus infection in diagnostic laboratories and monitor immunological protection in vaccine recipients. Wild-type (wt) viruses used in the PRNT may be difficult to grow and plaque titrate, such as the dengue viruses (DENV), and/or may ...
Barbara W, Johnson +6 more
openaire +2 more sources
Graz Reading Comprehension Test
Der GraLeV ist ein Verfahren zur Messung des Leseverständnisses (auf Wort-, Satz- und Textebene) von Kindern der dritten und vierten Schulstufe. Er besteht aus 4 Subtests mit insgesamt 38 Itemsets. Der GraLeV weist eine interne Konsistenz von Cronbachs α
Riedl, S. +4 more
core +1 more source
Background Dengue is a global public health challenge which requires accurate diagnostic methods for surveillance and control. The gold standard for detecting dengue neutralizing antibodies (nAbs) is the plaque reduction neutralization test (PRNT), which
Vanessa Shi Li Goh +5 more
doaj +1 more source
Diagnosis of arboviruses using indirect sandwich IgG ELISA in horses from the Brazilian Amazon
Background The Amazon as a whole is the largest reservoir of arboviruses worldwide, while the Brazilian Amazon hosts the largest variety of arboviruses isolated to date.
Alexandre do Rosário Casseb +8 more
doaj +1 more source
Comparison of Plaque- and Flow Cytometry-Based Methods for Measuring Dengue Virus Neutralization [PDF]
As dengue vaccines enter clinical trials, there is a need for rapid and quantitative assays to measure neutralization. We have developed flow-based neutralization assays which generated results similar to those generated by the established, plaque ...
Laura B. Haymore +7 more
core +1 more source
Serological Evidence of Orthopoxvirus Infection in Neotropical Primates in Brazil
The genus Orthopoxvirus (OPXV) of the family Poxviridae comprises several viruses that are capable of infecting a wide range of hosts. One of the most widespread OPXVs is the Vaccinia virus (VACV), which circulates in zoonotic cycles in South America ...
Filipe Vieira Santos de Abreu +24 more
doaj +1 more source
Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection. [PDF]
Zika virus (ZIKV) is an emerging arbovirus belonging to the genus flavivirus that comprises other important public health viruses, such as dengue (DENV) and yellow fever (YFV).
Andrea Cristine Koishi +5 more
doaj +1 more source
Deciphering Antibody Responses to Orthonairoviruses in Ruminants
Antibody cross-reactivities between related viruses are common diagnostic challenges, resulting in reduced diagnostic specificities and sensitivities. In this study, antibody cross-reactions between neglected members of the genus Orthonairovirus—Hazara ...
Julia Hartlaub +2 more
doaj +1 more source
Comparison of commercial enzyme immunoassay kits with plaque reduction neutralization test for detection of measles virus antibody [PDF]
Four commercially available enzyme immunoassay (EIA) kits were evaluated in comparison with the plaque reduction neutralization (PRN) test for detection of measles virus antibody. The EIA kits, Enzygnost (Behring), Diamedix, Vidas (bioMerieux Vitek), and Measlestat (Biowhittaker), were assessed with two PRN cutoff titers: a PRN titer of 8, the lowest ...
S, Ratnam +6 more
openaire +2 more sources

