Validation and Establishment of the SARS-CoV-2 Lentivirus Surrogate Neutralization Assay as a Prescreening Tool for the Plaque Reduction Neutralization Test [PDF]
Microbiology Spectrum, 2023 Neutralization assays are important for understanding and quantifying neutralizing antibody responses toward severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
John Merluza +10 more
doaj +6 more sources
Viruses, 2023 Since their first documentation in 1952, plaque reduction neutralization tests (PRNTs) have become the choice of test for the measurement of neutralizing antibodies against a particular virus.
Sunil R. Vaidya
doaj +5 more sources
Evaluation of a SARS-CoV-2 lateral flow assay using the plaque reduction neutralization test [PDF]
Diagnostic Microbiology and Infectious Disease, 2021 As new tests and technologies advance our understanding and diagnostic capabilities of the severe acute respiratory syndrome coronavirus 2 and the coronavirus disease 2019, they must be appropriately validated to make sure test performance is following manufacturer claims. In this study, we evaluated the Vazyme 2019-nCoV IgG/IgM Detection Kit, which is
Marisa Nielsen, Ping Ren, Xuping Xie
exaly +4 more sources
Pseudo-plaque reduction neutralization test (PPRNT) for the measurement of neutralizing antibodies to Crimean-Congo hemorrhagic fever virus [PDF]
Virology Journal, 2013 Background Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus of the genus Nairovirus family Bunyaviridae, which are enveloped viruses containing tripartite, negative polarity, single-stranded RNA.
Canakoglu Nurettin +8 more
doaj +7 more sources
Plaque Reduction Neutralization Test (PRNT) Accuracy in Evaluating Humoral Immune Response to SARS-CoV-2 [PDF]
DiseasesMassive vaccination positively impacted the SARS-CoV-2 pandemic, being a strategy to increase the titers of neutralizing antibodies (NAbs) in the population.
Ingrid Siciliano Horbach +23 more
doaj +4 more sources
Evaluation of a commercial ELISA as alternative to plaque reduction neutralization test to detect neutralizing antibodies against SARS-CoV-2 [PDF]
Scientific Reports, 2022 High-throughput detection of neutralizing antibodies against SARS-CoV-2 presents a valuable tool for vaccine trials or investigations of population immunity.
Natalie Hofmann +4 more
doaj +7 more sources
Evaluating Humoral Immunity against SARS-CoV-2: Validation of a Plaque-Reduction Neutralization Test and a Multilaboratory Comparison of Conventional and Surrogate Neutralization Assays [PDF]
Microbiology Spectrum, 2021 The evaluation of humoral protective immunity against SARS-CoV-2 remains crucial in understanding both natural immunity and protective immunity conferred by the several vaccines implemented in the fight against COVID-19.
Emelissa J. Valcourt +30 more
doaj +2 more sources
Performance assessment of SARS-CoV-2 IgM & IgG ELISAs in comparison with plaque reduction neutralization test. [PDF]
Indian J Med Res, 2021 Background & objectives: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) continues to be a devastating pandemic. This study was aimed at performance assessment of SARS-CoV-2 IgM and IgG ELISAs, and investigation of their utility for patient diagnosis and sero ...
Kulkarni R +8 more
europepmc +3 more sources
Journal of Virological Methods, 2022 Japanese encephalitis is prevalent throughout the temperate and tropical regions of Asia and is caused by the Japanese encephalitis virus (JEV), a mosquito-borne viral pathogen. The plaque reduction neutralization test (PRNT) is currently recommended as the gold standard test for detecting human antibodies against JEV.
Younhee Park, Jung Sik, Yun-Ho Hwang
exaly +4 more sources
Comparison of JEV neutralization assay using pseudotyped JEV with the conventional plaque-reduction neutralization test. [PDF]
J Microbiol, 2014 We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV-PV). JEV-PV-based neutralization assay offers several advantages compared with the current standard plaque-reduction neutralization test (PRNT), including simplicity, safety, and
Lee HJ +7 more
europepmc +4 more sources