Variability in dengue titer estimates from plaque reduction neutralization tests poses a challenge to epidemiological studies and vaccine development. [PDF]
PLoS Neglected Tropical Diseases, 2014 BACKGROUND:Accurate determination of neutralization antibody titers supports epidemiological studies of dengue virus transmission and vaccine trials. Neutralization titers measured using the plaque reduction neutralization test (PRNT) are believed to ...
Henrik Salje +9 more
doaj +6 more sources
Onderstepoort Journal of Veterinary Research, 2004 Blood samples from 50 dogs were collected at three veterinary clinics in Ibadan and Abuja, Nigeria and the serum from each sample was evaluated serologically for neutralizing antibodies against canine distemper virus (CDV) by the highly sensitive ...
O.I. Oyedele +3 more
doaj +2 more sources
Comparison of JEV neutralization assay using pseudotyped JEV with the conventional plaque-reduction neutralization test. [PDF]
J Microbiol, 2014 We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV-PV). JEV-PV-based neutralization assay offers several advantages compared with the current standard plaque-reduction neutralization test (PRNT), including simplicity, safety, and
Lee HJ +7 more
europepmc +4 more sources
Plaque Reduction Neutralization Test (PRNT) in the Congenital Zika Syndrome: Positivity and Associations with Laboratory, Clinical, and Imaging Characteristics [PDF]
Viruses, 2020 The short duration of viremia, low blood viral load, inaccessibility to timely specific diagnostic tests, and cross-reactions with other flaviviruses have hindered laboratory confirmation of Congenital Zika Syndrome (CZS).
Marizélia R. C. Ribeiro +16 more
doaj +2 more sources
Assessment of Mumps Virus-Specific Antibodies: Comparison of Plaque Reduction Neutralization Test and Enzyme-Linked Immunosorbent Assay Estimates [PDF]
Journal of Infectious Diseases, 2019 Paul Gillard +2 more
exaly +2 more sources
Varicella-zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique [PDF]
Journal of Clinical Microbiology, 1976 A new plaque assay for the quantitation of varicella-zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed using the indirect immunoperoxidase technique. As compared with the classical plaque assay using a solid overlay, the test gives earlier results since plaque counting can ...
G, Gerna, R W, Chambers
openaire +2 more sources
Biomedicines, 2022 (Background) The coronavirus disease 2019 (COVID-19) that is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) carries high infectivity and mortality. Efficient intervention strategies are urgently needed. Avian immunoglobulin Y (IgY)
Chia-Tsui Yeh +12 more
doaj +1 more source
Development of a High-Content Orthopoxvirus Infectivity and Neutralization Assays. [PDF]
PLoS ONE, 2015 Currently, a number of assays measure Orthopoxvirus neutralization with serum from individuals, vaccinated against smallpox. In addition to the traditional plaque reduction neutralization test (PRNT), newer higher throughput assays are based on ...
Irina Gates +5 more
doaj +1 more source
ZIKV NS1 IgG3 assay, DENV NS1 total IgG assay and Plaque reduction neutralization test (PRNT).
, 2023 ZIKV NS1 IgG3 assay, DENV NS1 total IgG assay and Plaque reduction neutralization test (PRNT).
Carlos Frederico C. A. Melo (16484797) +13 more
core +1 more source
Viruses, 2022 A robust serological test to measure neutralizing antibodies against SARS-CoV-2 in biosafety level-2 (BSL-2) laboratories is useful for monitoring antibody response after vaccination or natural infection.
Jing Zou +4 more
doaj +1 more source