Plaque Reduction Neutralization Test (PRNT) in the Congenital Zika Syndrome: Positivity and Associations with Laboratory, Clinical, and Imaging Characteristics [PDF]
Viruses, 2020 The short duration of viremia, low blood viral load, inaccessibility to timely specific diagnostic tests, and cross-reactions with other flaviviruses have hindered laboratory confirmation of Congenital Zika Syndrome (CZS).
Marizélia R. C. Ribeiro +16 more
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Variability in dengue titer estimates from plaque reduction neutralization tests poses a challenge to epidemiological studies and vaccine development. [PDF]
PLoS Neglected Tropical Diseases, 2014 BACKGROUND:Accurate determination of neutralization antibody titers supports epidemiological studies of dengue virus transmission and vaccine trials. Neutralization titers measured using the plaque reduction neutralization test (PRNT) are believed to ...
Henrik Salje +9 more
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Assessment of Mumps Virus-Specific Antibodies: Comparison of Plaque Reduction Neutralization Test and Enzyme-Linked Immunosorbent Assay Estimates [PDF]
Journal of Infectious Diseases, 2019 Paul Gillard +2 more
exaly +2 more sources
Varicella-zoster plaque assay and plaque reduction neutralization test by the immunoperoxidase technique [PDF]
Journal of Clinical Microbiology, 1976 A new plaque assay for the quantitation of varicella-zoster virus and a plaque reduction neutralization test for the determination of neutralizing antibody titer have been developed using the indirect immunoperoxidase technique. As compared with the classical plaque assay using a solid overlay, the test gives earlier results since plaque counting can ...
G, Gerna, R W, Chambers
openaire +2 more sources
Biomedicines, 2022 (Background) The coronavirus disease 2019 (COVID-19) that is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) carries high infectivity and mortality. Efficient intervention strategies are urgently needed. Avian immunoglobulin Y (IgY)
Chia-Tsui Yeh +12 more
doaj +1 more source
Development of a High-Content Orthopoxvirus Infectivity and Neutralization Assays. [PDF]
PLoS ONE, 2015 Currently, a number of assays measure Orthopoxvirus neutralization with serum from individuals, vaccinated against smallpox. In addition to the traditional plaque reduction neutralization test (PRNT), newer higher throughput assays are based on ...
Irina Gates +5 more
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Viruses, 2022 A robust serological test to measure neutralizing antibodies against SARS-CoV-2 in biosafety level-2 (BSL-2) laboratories is useful for monitoring antibody response after vaccination or natural infection.
Jing Zou +4 more
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International Journal of Infectious Diseases, 2023 Objectives: Regarding reactogenicity and immunogenicity, heterologous COVID-19 vaccination regimens are considered as an alternative to conventional immunization schemes.
Niko Kohmer +9 more
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Comparison of serological assays to titrate Hantaan and Seoul hantavirus-specific antibodies
Virology Journal, 2017 Background Hantaan and Seoul viruses, in the Hantavirus genus, are known to cause hemorrhagic fever with renal syndrome (HFRS). The plaque reduction neutralization test (PRNT), as conventional neutralization test for hantaviruses, is laborious and time ...
Weihong Li +9 more
doaj +1 more source
Viridot: An automated virus plaque (immunofocus) counter for the measurement of serological neutralizing responses with application to dengue virus. [PDF]
PLoS Neglected Tropical Diseases, 2018 The gold-standard method for quantifying neutralizing antibody responses to many viruses, including dengue virus (DENV), is the plaque reduction neutralization test (PRNT, also called the immunofocus reduction neutralization test).
Leah C Katzelnick +12 more
doaj +1 more source