Results 11 to 20 of about 29,718 (289)

Variation in dengue virus plaque reduction neutralization testing: systematic review and pooled analysis [PDF]

BMC Infectious Diseases, 2012
AbstractBackgroundThe plaque reduction neutralization test (PRNT) remains the gold standard for the detection of serologic immune responses to dengue virus (DENV). While the basic concept of the PRNT remains constant, this test has evolved in multiple laboratories, introducing variation in materials and methods.
Kaitlin Rainwater‐Lovett, Isabel Rodríguez-Barraquer, Derek A. T. Cummings, Justin Lessler   +3 more
core   +12 more sources

Serotypic classification of hantaviruses by indirect immunofluorescent antibody and plaque reduction neutralization tests [PDF]

Journal of Clinical Microbiology, 1985
Antisera prepared against 16 strains of hantaviruses isolated from patients with hemorrhagic fever with renal syndrome (HFRS) or from rodents captured in HFRS-endemic and nonendemic regions were titrated against Hantaan virus strain 76-118, Puumala virus strain Sotkamo, and Prospect Hill virus strain Prospect Hill-I by using the indirect ...
P W Lee, Clarence J. Gibbs, D. Carleton Gajdusek, Richard Yanagihara   +3 more
openalex   +3 more sources

Performance assessment of SARS-CoV-2 IgM & IgG ELISAs in comparison with plaque reduction neutralization test. [PDF]

Indian J Med Res, 2021
Background & objectives: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) continues to be a devastating pandemic. This study was aimed at performance assessment of SARS-CoV-2 IgM and IgG ELISAs, and investigation of their utility for patient diagnosis and sero ...
Kulkarni R, Shrivastava S, Patil HP, Kore P, Rane P, Palkar S, Lalwani S, Mishra AC, Arankalle VA.   +8 more
europepmc   +3 more sources

Utility of an In-Vitro Micro-Neutralizing Test in Comparison to a Plaque Reduction Neutralization Test for Dengue Virus, Japanese Encephalitis Virus, and Zika Virus Serology and Drug Screening. [PDF]

Pathogens, 2023
Haga K, Chen ZN, Himeno M, Majima R, Moi ML.   +4 more
europepmc   +3 more sources

Bluetongue Virus: (1) In Pregnant White-tailed Deer (2) A Plaque Reduction Neutralization Test [PDF]

Journal of Wildlife Diseases, 1970
Six white-tailed deer (Odocoileus virginianus) were infected with bluetongue virus (BTs, vaccinal strain) approximately one-third of the way through their gestation period. One deer died of bluetongue 21 days after inoculation. Of the five surviving the infection, one had two mummified fetuses, and the others no fetuses upon euthanasia two weeks after ...
F C Thomas, D. O. Trainer
openalex   +3 more sources

Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization [PDF]

Journal of Clinical Microbiology, 1985
A newly modified semimicro plaque reduction neutralization test (PRNT) in BHK cells was compared with a standard PRNT in bottles with LLC-MK2 monolayers and with an LLC-MK2 PRNT adapted to semimicro methods. The BHK semimicro PRNT compared favorably in terms of sensitivity in detecting dengue antibody (96%), specificity at a screening dilution (95 ...
David M. Morens, S B Halstead, Patricia M. Repik, Ravithat Putvatana, N Raybourne   +4 more
  +6 more sources

Plaque Reduction Neutralization Test for Smallpox Vaccines: Laboratory Optimization and Validation Method for Immunogenicity Assessment

Journal of Immunological Methods
The eradication of smallpox, a historic triumph in global public health, was accomplished without a complete conception of the mechanisms underlying vaccine-induced protection. Contemporary concerns regarding potential bioterrorism threats and the possibility of smallpox reemergence have spurred research efforts toward developing third-generation ...
Hyun-Jung Kong, You-Jin Kim, Dokeun Kim, Yun-Ho Hwang   +3 more
  +5 more sources

Variability in dengue titer estimates from plaque reduction neutralization tests poses a challenge to epidemiological studies and vaccine development. [PDF]

PLoS Neglected Tropical Diseases, 2014
BACKGROUND:Accurate determination of neutralization antibody titers supports epidemiological studies of dengue virus transmission and vaccine trials. Neutralization titers measured using the plaque reduction neutralization test (PRNT) are believed to ...
Henrik Salje, Isabel Rodríguez-Barraquer, Kaitlin Rainwater-Lovett, Ananda Nisalak, Butsaya Thaisomboonsuk, Stephen J Thomas, Stefan Fernandez, Richard G Jarman, In-Kyu Yoon, Derek A T Cummings   +9 more
doaj   +3 more sources

Loss of repeat convalescent plasma donors due to waning anti-SARS-CoV-2 plaque reduction neutralization test titers (April-December 2020) [PDF]

Transfus Med Rev, 2022
Steven J. Drews, Dana V. Devine, Janet McManus, Emelissa J. Mendoza, Kathy Manguiat, Roxie C. Girardin, Alan P. Dupuis, Kathleen A. McDonough, James A. Lin, David T. Evans, Chantale Pambrun, Michael Drebot, Heidi Wood   +12 more
europepmc   +3 more sources

Evaluation of the focus reduction neutralization and ELISA tests compared to the plaque reduction neutralization test for the detection of antibodies against measles virus [PDF]

, 2022
Abstract Measles is an infectious febrile sickness. Despite the existence of effective vaccination, eradicating measles remains a global priority. It is required to set up a sensitive and specific test to identify measles-specific antibodies in order to estimate the level of community protection against measles.
somayeh yaghoobizad, Zahra Norouzbabaei, Nazanin Zahra Shafiei Jandaghi, Abbas Rahimi Foroushani, Kaveh Sadeghi, Shahrokh Izadi, Elaheh Heidari, Vahid Salimi, Talat Mokhtari‐Azad   +8 more
openalex   +2 more sources