Results 1 to 10 of about 1,008,064 (370)

Cloning and expression of Toxoplasma gondii GRA-4 recombinant protein as a toxoplasmosis diagnostic kit candidate [PDF]

Veterinary World, 2020
Aim: The objective of this study was to produce recombinant protein GRA-4 (rGRA-4) of a local Toxoplasma gondii isolate as a candidate for a toxoplasmosis diagnosis kit in Escherichia coli BL21 (DE3) competent cells using pET SUMO plasmid.
Muhammad Hanafiah, Teuku Zahrial Helmi, Amalia Sutriana, Dwi Priyowidodo, Fihiruddin Fihiruddin   +4 more
doaj   +1 more source

Co-occurrence of blaNDM–1 and mcr-9 in a Conjugative IncHI2/HI2A Plasmid From a Bloodstream Infection-Causing Carbapenem-Resistant Klebsiella pneumoniae

Frontiers in Microbiology, 2021
Spread of the carbapenemase-encoding and mobilized colistin resistance (mcr) genes among Enterobacteriales poses a great threat to global public health, especially when the both genes are transferred by a single plasmid.
Zhou Liu, Xiubing Hang, Xiao Xiao, Wenwen Chu, Xin Li, Yangyang Liu, Yangyang Liu, Yangyang Liu, Xi Li, Qiang Zhou, Jiabin Li, Jiabin Li, Jiabin Li, Jiabin Li   +13 more
doaj   +1 more source

Molecular Analysis of blaKPC-2-Harboring Plasmids: Tn4401a Interplasmid Transposition and Tn4401a-Carrying ColRNAI Plasmid Mobilization from Klebsiella pneumoniae to Citrobacter europaeus and Morganella morganii in a Single Patient

mSphere, 2021
The spread of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacterales is a public health concern. KPC-encoding blaKPC is predominantly spread by strains of a particular phylogenetic lineage, clonal group 258, but can also be spread by ...
Kayoko Sugita, Kotaro Aoki, Kohji Komori, Tatsuya Nagasawa, Yoshikazu Ishii, Satoshi Iwata, Kazuhiro Tateda   +6 more
doaj   +1 more source

Bacteria have numerous distinctive groups of phage–plasmids with conserved phage and variable plasmid gene repertoires

Nucleic Acids Research, 2021
Plasmids and temperate phages are key contributors to bacterial evolution. They are usually regarded as very distinct. However, some elements, termed phage–plasmids, are known to be both plasmids and phages, e.g. P1, N15 or SSU5. The number, distribution,
E. Pfeifer, J. A. Moura de Sousa, M. Touchon, E. Rocha   +3 more
semanticscholar   +1 more source

Genetic Diversity and Characteristics of blaNDM-Positive Plasmids in Escherichia coli

Frontiers in Microbiology, 2021
New Delhi metallo-β-lactamases (NDMs), including at least 28 variants, are a rapidly emerging family of β-lactamases worldwide, with a variety of infections caused by NDM-positive strains usually associated with very poor prognosis and high mortality ...
Zhiren Zhang, Hengzhao Guo, Xiaodong Li, Wenting Li, Guang Yang, Wenjun Ni, Meixiao Zhan, Meixiao Zhan, Ligong Lu, Ligong Lu, Zhenlin Zhang, Xiaobin Li, Zhiling Zhou   +12 more
doaj   +1 more source

Distribution and Transmission of Colistin Resistance Genes mcr-1 and mcr-3 among Nontyphoidal Salmonella Isolates in China from 2011 to 2020

Microbiology Spectrum, 2023
Mobile colistin resistance (mcr) genes are present mainly in plasmids and can disseminate clonally or horizontally via either plasmids or insertion sequences in different genomic locations among the Enterobacteriaceae.
Tingting Yang, Weiwei Li, Qingpo Cui, Xiaoxia Qin, Bosheng Li, Xiugui Li, Huayun Jia, Xiaorong Yang, Chengwei Liu, Yang Wang, Shaolin Wang, Jianzhong Shen, Yunchang Guo, Zhangqi Shen   +13 more
doaj   +1 more source

Characterization of an International High-Risk Escherichia coli ST410 Clone Coproducing NDM-5 and OXA-181 in a Food Market in China

Microbiology Spectrum, 2023
During a 2020 routine epidemiological investigation of carbapenem-resistant Enterobacterales at a local food market in Guangzhou, China, two Escherichia coli ST410 isolates coproducing NDM-5 and OXA-181 were obtained from environmental samples ...
Wan-Yun He, Lu-Chao Lv, Wen-Xian Pu, Guo-Long Gao, Zi-Lin Zhuang, Yao-Yao Lu, Chao Zhuo, Jian-Hua Liu   +7 more
doaj   +1 more source

DNA Minicircle Technology Improves Purity of Adeno-associated Viral Vector Preparations

Molecular Therapy: Nucleic Acids, 2016
Adeno-associated viral (AAV) vectors are considered as one of the most promising delivery systems in human gene therapy. In addition, AAV vectors are frequently applied tools in preclinical and basic research. Despite this success, manufacturing pure AAV
Maria Schnödt, Marco Schmeer, Barbara Kracher, Christa Krüsemann, Laura Escalona Espinosa, Anja Grünert, Thomas Fuchsluger, Anja Rischmüller, Martin Schleef, Hildegard Büning   +9 more
doaj   +1 more source

Subinhibitory Concentration of Colistin Promotes the Conjugation Frequencies of Mcr-1- and blaNDM-5-Positive Plasmids

Microbiology Spectrum, 2022
Horizontal gene transfer (HGT) plays a significant role in the spread of antibiotic resistance genes (ARGs). Most reported compounds promote HGT by increasing the cell membrane permeability.
Xia Xiao, Fuxin Zeng, Ruichao Li, Yuan Liu, Zhiqiang Wang   +4 more
doaj   +1 more source

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Nucleic Acids Research, 1979
A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction ...
H. Birnboim, J. Doly
semanticscholar   +1 more source